示差折光-液相色谱法测定蜂蜜中<em>β</em>-呋喃果糖苷酶残留量

引用本文: 王艳, 吴斌, 张晓燕, 沈崇钰, 张睿, 李丽花, 费晓庆, 赵增运. 示差折光-液相色谱法测定蜂蜜中β-呋喃果糖苷酶残留量[J]. 分析化学, 2012, 40(10): 1602-1606. doi: 10.3724/SP.J.1096.2012.20111 shu

Citation: WANG Yan, WU Bin, ZHANG Xiao-Yan, SHEN Chong-Yu, ZHANG Rui, LI Li-Hua, FEI Xiao-Qing, ZHAO Zeng-Yun. Determination of β-Fructofuranosidase by Liquid Chromatography with Refractive Index Detector[J]. Chinese Journal of Analytical Chemistry, 2012, 40(10): 1602-1606. doi: 10.3724/SP.J.1096.2012.20111 shu

示差折光-液相色谱法测定蜂蜜中β-呋喃果糖苷酶残留量

通讯作者: 王艳

摘要: 基于β-呋喃果糖苷酶与棉子糖在柠檬酸-氢氧化钠缓冲介质(pH4.50)中50℃下反应16 h后的酶解产物蜜二糖含量的变化,建立了高效液相色谱示差折光检测(HPLC-RID)技术测定蜂蜜中β-呋喃果糖苷酶残留量的新方法。以Agilent Carbohydrate柱为分离柱,乙腈-水(78:22, V/V)为流动相,流速为1.4 mL/min,检测器为示差折光检测器,柱温和检测器温度均为35℃。结果表明,蜂蜜样品经过聚丙烯酰胺凝胶柱分离后,样品峰形得到简化,灵敏度提高了1.3倍;方法的线性范围为10-200 U/kg;测出限为10 U/kg;回收率为81.3%-112.4%;相对标准偏差为3.7%-9.7%(n＝6)。当蜂蜜样品中β-呋喃果糖苷酶含量＞20 U/kg时,判定为阳性样品,即掺假蜂蜜。

关键词:

English

Determination of β-Fructofuranosidase by Liquid Chromatography with Refractive Index Detector

1.
Laboratory of Food, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, China

Corresponding author: 王艳

Received Date: 10 February 2012

Abstract: A new method for the determination of detecting β-fructofruanosidase in honey was developed by liquid chromatography with refractive index detector (HPLC-RID). The method was based on the changes of product concentration of melibiose by the reaction of β-fructofruanosidase enzyme with raffinose in the medium of citric acid-sodium hydroxide solution (pH＝4.50) for 16 h in water bath at 50℃. Agilent Carbohydrate was chosen as the separation column, acetonitrile-water (78:22, V/V) was selected as mobile phase with flow rate of 1.4 mL/min. The column temperature and detector temperature was 35℃. The results showed that the numbers of peaks were simplified and the sensitivity of this proposed method was improved 1.3 fold as well by using polyacrylamide gel column for pre-separation. A good linear fit was received for the concentration of β-fructofruanosidase enzyme from 10 U/kg to 200 U/kg with the detection limit of 10 U/kg. Recovery was obtained between 81.3% and 112.4% with the relative standard deviation of 3.7% to 9.7% (n＝6). It is judged to be adulteration honey if β-fructofruanosidase concentration is more than 20 U/kg,

Key words:

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

示差折光-液相色谱法测定蜂蜜中β-呋喃果糖苷酶残留量

通讯作者: 王艳

English

Determination of β-Fructofuranosidase by Liquid Chromatography with Refractive Index Detector

Corresponding author: 王艳

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

示差折光-液相色谱法测定蜂蜜中β-呋喃果糖苷酶残留量

通讯作者: 王艳

English

Determination of β-Fructofuranosidase by Liquid Chromatography with Refractive Index Detector

Corresponding author: 王艳

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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