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重金属Cr(Ⅵ)与血清蛋白质组的结合反应性能研究

郭明 ,  黄凤琴 ,  刘咪咪 ,  李铭慧

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引用本文: 郭明, 黄凤琴, 刘咪咪, 李铭慧. 重金属Cr(Ⅵ)与血清蛋白质组的结合反应性能研究[J]. 无机化学学报, 2013, 29(5): 1037-1044. doi: 10.3969/j.issn.1001-4861.2013.00.159 shu
Citation:  GUO Ming, HUANG Feng-Qin, LIU Mi-Mi, LI Ming-Hui. Performance of Binding Interaction between Cr(Ⅵ) and Serum Proteome[J]. Chinese Journal of Inorganic Chemistry, 2013, 29(5): 1037-1044. doi: 10.3969/j.issn.1001-4861.2013.00.159 shu

重金属Cr(Ⅵ)与血清蛋白质组的结合反应性能研究

    通讯作者: 郭明,E-mail:guoming@zafu.edu.cn
  • 基金项目:

    国家自然科学基金(No.20877072) (No.20877072)

    浙江科技厅公益项目(No.2012C37055) 资助项目。 (No.2012C37055)

摘要: 利用亲和毛细管电泳(Affinity capillary electrophoresis, ACE)方法,基于位点结合模型,构建配体(Cr(Ⅵ))-血清蛋白质组受体(Fetal bovine serum, FBS)结合反应方程,研究重金属离子Cr(Ⅵ)和血清蛋白质组的结合反应性能。结果表明,组学条件下,Cr(Ⅵ)与FBS中高丰度组分白蛋白(Bovine serum albumin, BSA)、转铁蛋白(Bovine apo transferrin, bATF)、免疫球蛋白(Bovine IgG, IgG)之间形成Cr(Ⅵ)-蛋白质络合物;结合反应均为快平衡反应;依据有效淌度的变化,通过结合方程测得结合反应的表观结合常数KCr(Ⅵ)-bATF=1.27×104 L·mol-1、KCr(Ⅵ)-IgG=4.91×104 L·mol-1、KCr(Ⅵ)-BSA=8.98×104 L·mol-1;同时根据实验数据非线性模拟结果表明,Cr(Ⅵ)与FBS中高丰度组分发生相互作用的强度与Cr(Ⅵ)浓度之间存在明显的量效关系。本文有关工作可为重金属离子-蛋白质络合物结合性能等生物无机化学研究内容提供参考。

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  • 收稿日期:  2012-12-25
  • 网络出版日期:  2013-01-31
通讯作者: 陈斌, bchen63@163.com
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