基于氢键作用的金纳米粒子比色法检测尿酸

引用本文: 鲁立强, 王晗, 曹怀元, 郄浩飞, 王明涛, 李勇, 田熙科. 基于氢键作用的金纳米粒子比色法检测尿酸[J]. 大学化学, 2020, 35(4): 173-177. doi: 10.3866/PKU.DXHX201912004 shu

Citation: Liqiang Lu, Han Wang, Huaiyuan Cao, Haofei Qie, Mingtao Wang, Yong Li, Xike Tian. Determination of Uric Acid by Gold Nanoparticles Colorimetry Based on Hydrogen Bonding[J]. University Chemistry, 2020, 35(4): 173-177. doi: 10.3866/PKU.DXHX201912004 shu

基于氢键作用的金纳米粒子比色法检测尿酸

中国地质大学(武汉)材料与化学学院, 武汉 430074
基金项目:
中国地质大学（武汉）本科教学改革研究项目

摘要: 尿酸含量高可使人产生痛风等疾病，尿酸的测定是临床检测重要的生化指标之一。金纳米粒子比色法检测尿酸实验联系实际生活，将科研前沿和教学内容有机结合起来，可以激发学生的学习兴趣，加深学生对经典理论的理解，增加学生对科研前沿的了解。本实验利用金纳米粒子吸光系数高的特点，通过尿酸与三聚氰胺反应后，抑制三聚氰胺诱导的金纳米粒子聚集，从而达到检测尿酸的目的。随着溶液中尿酸浓度的增加，溶液颜色由蓝变红，差别明显，视觉效果好，容易分辨。

关键词:

English

Determination of Uric Acid by Gold Nanoparticles Colorimetry Based on Hydrogen Bonding

Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, P. R. China
Received Date: 02 December 2019

Abstract: High content of uric acid can cause gout and other diseases, and thus uric acid is one of the important biochemical indicators in clinical test. Detection of uric acid based on gold nanoparticle colorimetry combines the forefront of scientific research with the content of teaching, which can stimulate students' interest in learning, deepen their understanding of classical theories and increase their understanding of the forefront of scientific research. In this experiment, gold nanoparticles were used to detect uric acid by inhibiting the accumulation of gold nanoparticles induced by melamine after the reaction of uric acid with melamine. With the increase of the concentration of uric acid in the solution, the color of the solution changes from blue to red, and the difference is obvious. The visual effect is good and easy to observe.

Key words:

1. [1] Pasalic, D.; Marinkovic, N.; Feher, T. L. Biochem. Med. (Zagreb) 2012, 22, 63.
2. [2] 胡晶, 祝蕾, 王珍妮, 王佩瑶, 杨梅. 广州化工, 2013, 41 (8), 110.
3. [3] Piermarini, S.; Migliorelli, D.; Volpe, G.; Massoud, R.; Palleschi, G. Sensor. Actuat. B-Chem. 2013, 179, 170.
4. [4] Raj, C. R.; Ohsaka, T. J. Electroanal. Chem. 2003, 540, 69.
5. [5] Zhang, Z.; Yin, J. Electrochim. Acta 2014, 119, 32.
6. [6] Roushania, M.; Shamsipur, M. J. Electroanal. Chem. 2014, 712, 19.
7. [7] Zhang, T.; Sun, X.; Liu, B. Spectrochim. Acta A 2011, 79, 1566.
8. [8] Hasebe, Y.; Ujita, S. Analyst 1998, 123, 1775.
9. [9] Liu, Z.; Li, T.; Wang, E. Analyst 1995, 120, 2181.
10. [10] Lehn, J. M.; Mascal, M.; Cian, A. D.; Fischer, J. J. Chem. Soc., Chem. Commun. 1990, 6, 479.

扫一扫看文章

计量

PDF下载量: 9
文章访问数: 1223
HTML全文浏览量: 193

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于氢键作用的金纳米粒子比色法检测尿酸

English

Determination of Uric Acid by Gold Nanoparticles Colorimetry Based on Hydrogen Bonding

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于氢键作用的金纳米粒子比色法检测尿酸

English

Determination of Uric Acid by Gold Nanoparticles Colorimetry Based on Hydrogen Bonding

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs