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蛋白质组学中的固定化酶反应器制备的研究进展

张凌怡 ,  王兵兵 ,  上官露露 ,  张润生 ,  陈建虎 ,  张维冰

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引用本文: 张凌怡, 王兵兵, 上官露露, 张润生, 陈建虎, 张维冰. 蛋白质组学中的固定化酶反应器制备的研究进展[J]. 色谱, 2015, 33(11): 1121-1125. doi: 10.3724/SP.J.1123.2015.06028 shu
Citation:  ZHANG Lingyi, WANG Bingbing, SHANGGUAN Lulu, ZHANG Runsheng, CHEN Jianhu, ZHANG Weibing. Development of preparation of immobilized enzyme reactors in proteomics[J]. Chinese Journal of Chromatography, 2015, 33(11): 1121-1125. doi: 10.3724/SP.J.1123.2015.06028 shu

蛋白质组学中的固定化酶反应器制备的研究进展

    通讯作者: 张维冰
  • 基金项目:

    国家重大科学仪器设备开发专项(2012YQ120044) (2012YQ120044)

    上海市刑事科学技术研究院开放课题. 

摘要: 固定化酶反应器作为蛋白质组学研究中"bottom-up"策略重要的组件,具有酶解快速、酶解效率高、酶稳定性和活性高、简单易操作、能够与多种检测方式联用等优点,对于发展高效快速的蛋白质组学分析方法具有重要意义。本文就固定化酶反应器的制备方法及其在蛋白质组学中的应用做简单的概述,着重介绍酶的固定方法、固定化酶的载体、用于固定的酶的种类。近几年固定化酶反应器的研究集中于提高固酶量、保持酶活性、增加酶解效率、减小非特异性吸附等方面。研究结果表明,采用纳米材料、整体材料等新型载体,提高载体亲水性,采用多酶同时酶解等方法能够有效改善固定化酶反应器的性能,提高蛋白质的鉴定效率。

English

    1. [1] Switzar L, Giera M, Niessen W M A. J Proteome Res, 2013, 12(3): 1067  [1] Switzar L, Giera M, Niessen W M A. J Proteome Res, 2013, 12(3): 1067  

    2. [2] Compton P D, Zamdborg L, Thomas P M, et al. Anal Chem, 2011, 83(17): 6868  [2] Compton P D, Zamdborg L, Thomas P M, et al. Anal Chem, 2011, 83(17): 6868  

    3. [3] Hartmann M, Kostrov X. Chem Soc Rev, 2013, 42(15): 6277  [3] Hartmann M, Kostrov X. Chem Soc Rev, 2013, 42(15): 6277  

    4. [4] Magner E. Chem Soc Rev, 2013, 42(15): 6213  [4] Magner E. Chem Soc Rev, 2013, 42(15): 6213  

    5. [5] Sheldon R A, van Pelt S. Chem Soc Rev, 2013, 42(15): 6233[5] Sheldon R A, van Pelt S. Chem Soc Rev, 2013, 42(15): 6233

    6. [6] Lakouraj M M, Maashsani A, Norouzian R S, et al. J Carbohydr Chem, 2015, 34(2): 103  [6] Lakouraj M M, Maashsani A, Norouzian R S, et al. J Carbohydr Chem, 2015, 34(2): 103  

    7. [7] Bian Y Y, Ye M L, Song C X, et al. J Proteome Res, 2012, 11(5): 2828  [7] Bian Y Y, Ye M L, Song C X, et al. J Proteome Res, 2012, 11(5): 2828  

    8. [8] Shangguan L L, Zhang L Y, Xiong Z C, et al. J Chromatogr A, 2015, 1388: 158  [8] Shangguan L L, Zhang L Y, Xiong Z C, et al. J Chromatogr A, 2015, 1388: 158  

    9. [9] Ma J F. [PhD Dissertation]. Dalian: Dalian Institute of Chemical Physics, Chinese Academy of Sciences (马俊峰. [博士学位论文]. 大连: 中国科学院大连化学物理研究所), 2009[9] Ma J F. [PhD Dissertation]. Dalian: Dalian Institute of Chemical Physics, Chinese Academy of Sciences (马俊峰. [博士学位论文]. 大连: 中国科学院大连化学物理研究所), 2009

    10. [10] Temporini C, Calleri E, Cabrera K, et al. J Sep Sci, 2009, 32(8): 1120  [10] Temporini C, Calleri E, Cabrera K, et al. J Sep Sci, 2009, 32(8): 1120  

    11. [11] Jiang B, Yang K G, Zhao Q, et al. J Chromatogr A, 2012, 1254: 8  [11] Jiang B, Yang K G, Zhao Q, et al. J Chromatogr A, 2012, 1254: 8  

    12. [12] Yin Z R, Zhao W W, Tian M M, et al. Analyst, 2014, 1397(7): 1973[12] Yin Z R, Zhao W W, Tian M M, et al. Analyst, 2014, 1397(7): 1973

    13. [13] Min W N, Wang W P, Chen J R, et al. Anal Bioanal Chem, 2012, 404(8): 2397  [13] Min W N, Wang W P, Chen J R, et al. Anal Bioanal Chem, 2012, 404(8): 2397  

    14. [14] Ma J F, Hou C Y, Liang Y, et al. Proteomics, 2011, 11(5): 991  [14] Ma J F, Hou C Y, Liang Y, et al. Proteomics, 2011, 11(5): 991  

    15. [15] Zhou Z, Hartmann M. Top Catal, 2012, 55(16): 1081[15] Zhou Z, Hartmann M. Top Catal, 2012, 55(16): 1081

    16. [16] Li Y H, Wojcik R, Dovichi N J, et al. J Chromatogr A, 2011, 1218: 2007  [16] Li Y H, Wojcik R, Dovichi N J, et al. J Chromatogr A, 2011, 1218: 2007  

    17. [17] Sun L L, Li Y H, Yang P, et al. J Chromatogr A, 2012, 1220: 68  [17] Sun L L, Li Y H, Yang P, et al. J Chromatogr A, 2012, 1220: 68  

    18. [18] Bao H M, Zhang L Y, Chen G. J Chromatogr A, 2013, 1310: 74  [18] Bao H M, Zhang L Y, Chen G. J Chromatogr A, 2013, 1310: 74  

    19. [19] Yuan H M, Zhang L H, Zhang Y K. J Chromatogr A, 2014, 1371: 48  [19] Yuan H M, Zhang L H, Zhang Y K. J Chromatogr A, 2014, 1371: 48  

    20. [20] Foo H C, Smith W N, Stanley M R S. Talanta, 2015, 135: 18  [20] Foo H C, Smith W N, Stanley M R S. Talanta, 2015, 135: 18  

    21. [21] Sun L L, Zhu G J, Dovichi N J, et al. Anal Chem, 2013, 85(8): 4187  [21] Sun L L, Zhu G J, Dovichi N J, et al. Anal Chem, 2013, 85(8): 4187  

    22. [22] Liu F J, Ye M L, Wang C L, et al. Anal Chem, 2013, 85(15): 7024  [22] Liu F J, Ye M L, Wang C L, et al. Anal Chem, 2013, 85(15): 7024  

    23. [23] Qin W J, Song Z F, Fan C, et al. Anal Chem, 2012, 84(7): 3138  [23] Qin W J, Song Z F, Fan C, et al. Anal Chem, 2012, 84(7): 3138  

    24. [24] Fan C, Shi Z M, Pan Y T, et al. Anal Chem, 2014, 86(3): 1452  [24] Fan C, Shi Z M, Pan Y T, et al. Anal Chem, 2014, 86(3): 1452  

    25. [25] Mu X Y, Qiao J, Qi L, et al. Appl Mater Interfaces, 2014, 6(23): 21346  [25] Mu X Y, Qiao J, Qi L, et al. Appl Mater Interfaces, 2014, 6(23): 21346  

    26. [26] Ju H Y, Kuo C H, Too J R, et al. J Mol Catal B: Enzym, 2012, 78: 9  [26] Ju H Y, Kuo C H, Too J R, et al. J Mol Catal B: Enzym, 2012, 78: 9  

    27. [27] Malvi B, Gupta S S. Chem Commun, 2012, 48(63): 7853  [27] Malvi B, Gupta S S. Chem Commun, 2012, 48(63): 7853  

    28. [28] Zhou Z, Piepenbreier F, Marthala V R R, et al. Catal Today, 2015, 243: 173  [28] Zhou Z, Piepenbreier F, Marthala V R R, et al. Catal Today, 2015, 243: 173  

    29. [29] Arrua R D, Talebi M, Causon T J, et al. Anal Chim Acta, 2012, 738: 1  [29] Arrua R D, Talebi M, Causon T J, et al. Anal Chim Acta, 2012, 738: 1  

    30. [30] Connolly D, Currivan S, Paull B. Proteomics, 2012, 12(19): 1[30] Connolly D, Currivan S, Paull B. Proteomics, 2012, 12(19): 1

    31. [31] Walsh Z, Paull B, Macka M. Anal Chim Acta, 2012, 750: 28  [31] Walsh Z, Paull B, Macka M. Anal Chim Acta, 2012, 750: 28  

    32. [32] Rivera J G, Messersmith P B. J Sep Sci, 2012, 35(12): 1514  [32] Rivera J G, Messersmith P B. J Sep Sci, 2012, 35(12): 1514  

    33. [33] Krenkova J, Szekrenyes A, Keresztessy Z, et al. J Chromatogr A, 2013, 1322: 54  [33] Krenkova J, Szekrenyes A, Keresztessy Z, et al. J Chromatogr A, 2013, 1322: 54  

    34. [34] Ou J J, Lin H, Zhang Z B, et al. Electrophoresis, 2013, 34(1): 126  [34] Ou J J, Lin H, Zhang Z B, et al. Electrophoresis, 2013, 34(1): 126  

    35. [35] Yuan H M, Zhou Y, Xia S M, et al. Anal Chem, 2012, 84(11): 5124  [35] Yuan H M, Zhou Y, Xia S M, et al. Anal Chem, 2012, 84(11): 5124  

    36. [36] Zeng R J, Luo Z F, Zhou D, et al. Electrophoresis, 2010, 31(19): 3334  [36] Zeng R J, Luo Z F, Zhou D, et al. Electrophoresis, 2010, 31(19): 3334  

    37. [37] Jia M, Qin L, He X W, et al. J Mater Chem, 2012, 22: 707  [37] Jia M, Qin L, He X W, et al. J Mater Chem, 2012, 22: 707  

    38. [38] Jiang H, Yuan H M, Liang Y, et al. J Chromatogr A, 2012, 1246: 111  [38] Jiang H, Yuan H M, Liang Y, et al. J Chromatogr A, 2012, 1246: 111  

    39. [39] Schulze A, Marquardt B, Kaczmarek S, et al. Macromol Rapid Commun, 2010, 31(5): 467  [39] Schulze A, Marquardt B, Kaczmarek S, et al. Macromol Rapid Commun, 2010, 31(5): 467  

    40. [40] Schulze A, Marquardt B, Went M, et al. Water Sci Technol, 2012, 65(3): 574  [40] Schulze A, Marquardt B, Went M, et al. Water Sci Technol, 2012, 65(3): 574  

    41. [41] Schulze A, Maitz M F, Zimmermann R, et al. RSC Adv, 2013, 3(44): 22518  [41] Schulze A, Maitz M F, Zimmermann R, et al. RSC Adv, 2013, 3(44): 22518  

    42. [42] Starke S, Went M, Prager A, et al. React Funct Polym, 2013, 73(5): 698  [42] Starke S, Went M, Prager A, et al. React Funct Polym, 2013, 73(5): 698  

    43. [43] Feng Q, Zhao Y, Wei A F, et al. Environ Sci Technol, 2014, 48(17): 10390  [43] Feng Q, Zhao Y, Wei A F, et al. Environ Sci Technol, 2014, 48(17): 10390  

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  • 收稿日期:  2015-06-17
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