液相色谱-四极杆-飞行时间串联质谱法高通量分析美国红参的脑苷脂分子种

引用本文: 邢培培, 徐杰, 丛培旭, 王扬, 何中央, 薛长湖. 液相色谱-四极杆-飞行时间串联质谱法高通量分析美国红参的脑苷脂分子种[J]. 色谱, 2014, 32(1): 26-33. doi: 10.3724/SP.J.1123.2013.08002 shu

Citation: XING Peipei, XU Jie, CONG Peixu, WANG Yang, HE Zhongyang, XUE Changhu. High throughput analysis of cerebroside molecular species from sea cucumber Parastichopus californicus by liquid chromatography-quadrupole-time-of-flight mass spectrometry[J]. Chinese Journal of Chromatography, 2014, 32(1): 26-33. doi: 10.3724/SP.J.1123.2013.08002 shu

液相色谱-四极杆-飞行时间串联质谱法高通量分析美国红参的脑苷脂分子种

邢培培 ^1, ,
徐杰 ^{1,
,} ,
丛培旭 ^1, ,
王扬 ^2, ,
何中央 ^2, ,
薛长湖 ^1,

通讯作者: 徐杰

基金项目:
国家自然科学基金项目(31201329) (31201329)

国家“十二五”科技支撑计划项目(2012BAD33B07). (2012BAD33B07)

摘要: 利用液相色谱-四极杆-飞行时间串联质谱法(LC-Q-TOF MS/MS)分析美国红参(Parastichopus californicus)脑苷脂的分子种组成。以氯仿-甲醇(2:1,v/v)溶液提取,经皂化和固相萃取柱(SPE)净化后,在正离子模式下,通过自动二级质谱方式同时获取样品脑苷脂分子的一级和二级质谱图。筛取含180 Da中性丢失碎片的分子,再结合二级质谱中的长链碱和脂肪酸碎片,确定每种脑苷脂分子的结构。通过该法一次性共分析出123种美国红参脑苷脂分子种;含有的长链碱共18种,其中植物鞘氨醇型与鞘氨醇型长链碱的相对含量比为1:2;脂肪酸为18~25碳的饱和或单不饱和脂肪酸,以24碳脂肪酸为主,2-羟基脂肪酸占58.62%,饱和与单不饱和脂肪酸的相对含量比约为1:3。LC-Q-TOF MS/MS法分析海参脑苷脂分子种的灵敏度高,准确,简便,为海参脑苷脂的构效关系研究及功能食品开发提供理论依据。

关键词:

English

High throughput analysis of cerebroside molecular species from sea cucumber Parastichopus californicus by liquid chromatography-quadrupole-time-of-flight mass spectrometry

1.
1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;

Corresponding author: 徐杰

Received Date: 01 August 2013
Fund Project:
国家自然科学基金项目(31201329)

国家“十二五”科技支撑计划项目(2012BAD33B07).

Abstract: Cerebrosides from sea cucumber Parastichopus californicus were identified by using liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-Q-TOF MS/MS). The samples were extracted with chloroform-methanol (2:1, v/v) solution and purified by a SPE cartridge. In positive ion mode electrospray ionization (ESI), the precursor ion scan mass spectra and product ion scan mass spectra were obtained through the automatic MS/MS mode. Cerebroside molecules were selected according to the neutral loss fragments of 180 Da, and then were identified according to long-chain base (LCB) fragments and fatty acid (FA) fragments. One hundred and twenty-three cerebroside molecular species were identified. There are 18 species of LCB, and the relative content ratio of phytosphingosines and sphingosines is 1:2. The carbon numbers of fatty acids are mainly 18-25, of which 24 carbon fatty acids are predominant. The relative content ratio of saturated fatty acids and monounsaturated fatty acid is about 1:3, and the presence of 2-hydroxy fatty acids is about 58.62%. LC-Q-TOF MS/MS method is sensitive, accurate and simple. At the same time, this study provided a theoretical basis for structure-activity relationship studies and functional food development of Parastichopus californicus as well.

Key words:

liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-Q-TOF MS/MS)
/ cerebrosides
/ molecular species
/ Parastichopus californicus
/ high throughput analysis

1. [1] Chen Y, Lü J L, Duan J A, et al. Journal of International Pharmaceutical Research (陈颖, 吕洁丽, 段金廒, 等. 国际药学研究杂志), 2009, 36(2): 121[1] Chen Y, Lü J L, Duan J A, et al. Journal of International Pharmaceutical Research (陈颖, 吕洁丽, 段金廒, 等. 国际药学研究杂志), 2009, 36(2): 121
2. [2] Cha S, Yeung E S. Anal Chem, 2007, 79(6): 2373 [2] Cha S, Yeung E S. Anal Chem, 2007, 79(6): 2373
3. [3] Sarah T P, Bushnev A, Hagedorn K, et al. J Lipid Res, 2008, 49(8): 1621 [3] Sarah T P, Bushnev A, Hagedorn K, et al. J Lipid Res, 2008, 49(8): 1621
4. [4] Feng T Y, Xue C H, Xu J, et al. Chinese Journal of Pharmaceutical Analysis (冯婷玉, 薛长湖, 徐杰, 等. 药物分析杂志), 2011, 31(5): 896[4] Feng T Y, Xue C H, Xu J, et al. Chinese Journal of Pharmaceutical Analysis (冯婷玉, 薛长湖, 徐杰, 等. 药物分析杂志), 2011, 31(5): 896
5. [5] Xu J, Duan J J, Xue C H, et al. J Agric Food Chem, 2011, 59(22): 12246 [5] Xu J, Duan J J, Xue C H, et al. J Agric Food Chem, 2011, 59(22): 12246
6. [6] Sugawara T, Aida K, Duan J J, et al. J Oleo Sci, 2010, 59(7): 387 [6] Sugawara T, Aida K, Duan J J, et al. J Oleo Sci, 2010, 59(7): 387
7. [7] Guo S, Xue C H, Xu J, et al. Adv Mater Res, 2012, 524-527: 2334[7] Guo S, Xue C H, Xu J, et al. Adv Mater Res, 2012, 524-527: 2334
8. [8] Oku H, Li C C, Shimatani M, et al. Cancer Chemoth Pharm, 2009, 64(3): 485 [8] Oku H, Li C C, Shimatani M, et al. Cancer Chemoth Pharm, 2009, 64(3): 485
9. [9] Zhang B, Xue C H, Hu X Q, et al. Lipids Health Dis, 2012, 11(1): 48 [9] Zhang B, Xue C H, Hu X Q, et al. Lipids Health Dis, 2012, 11(1): 48
10. [10] Margalit M, Shalev Z, Pappo O, et al. J Pharmacol Exp, 2006, 319(1): 105 [10] Margalit M, Shalev Z, Pappo O, et al. J Pharmacol Exp, 2006, 319(1): 105
11. [11] Tang J, Meng X J, Liu H, et al. Molecules, 2010, 15(12): 9288 [11] Tang J, Meng X J, Liu H, et al. Molecules, 2010, 15(12): 9288
12. [12] Wang R F, Wu X W, Geng D. Molecules, 2013, 18(1): 1181 [12] Wang R F, Wu X W, Geng D. Molecules, 2013, 18(1): 1181
13. [13] Sugawara T, Kinoshita M, Ohnishi M, et al. Nutr Metab, 2003, 133(9): 2777[13] Sugawara T, Kinoshita M, Ohnishi M, et al. Nutr Metab, 2003, 133(9): 2777
14. [14] Sugawara T, Kinoshita M, Ohnishi M, et al. Biosci Biotech Bioch, 2004, 68(12): 2541 [14] Sugawara T, Kinoshita M, Ohnishi M, et al. Biosci Biotech Bioch, 2004, 68(12): 2541
15. [15] Cong P X, Li Z J, Xu J, et al. Chinese Journal of Chromatography (丛培旭, 李兆杰, 徐杰, 等. 色谱), 2013, 31(5): 399[15] Cong P X, Li Z J, Xu J, et al. Chinese Journal of Chromatography (丛培旭, 李兆杰, 徐杰, 等. 色谱), 2013, 31(5): 399
16. [16] Han X, Cheng H. J Lipid Res, 2005, 46(1): 163[16] Han X, Cheng H. J Lipid Res, 2005, 46(1): 163
17. [17] Leila H, Jason W H, Danni C, et al. Biochem J, 2011, 438(1): 165 [17] Leila H, Jason W H, Danni C, et al. Biochem J, 2011, 438(1): 165
18. [18] Sun L, Bi Y F, Zhang L, et al. Chinese Journal of Veterinary Drug (孙雷, 毕言锋, 张骊, 等. 中国兽药杂志), 2009, 43(7): 37[18] Sun L, Bi Y F, Zhang L, et al. Chinese Journal of Veterinary Drug (孙雷, 毕言锋, 张骊, 等. 中国兽药杂志), 2009, 43(7): 37
19. [19] Sperling P, Heinz E. Biochim Biophys Acta, 2003, 1632: 1 [19] Sperling P, Heinz E. Biochim Biophys Acta, 2003, 1632: 1
20. [20] Sugawara T, Zaima N, Yamamoto A. Biosci Biotech Bioch, 2006, 70(12): 2906 [20] Sugawara T, Zaima N, Yamamoto A. Biosci Biotech Bioch, 2006, 70(12): 2906
21. [21] Chrast R, Saher G, Nave K A, et al. J Lipid Res, 2011, 52(3): 419 [21] Chrast R, Saher G, Nave K A, et al. J Lipid Res, 2011, 52(3): 419

扫一扫看文章

计量

PDF下载量: 0
文章访问数: 634
HTML全文浏览量: 23

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

液相色谱-四极杆-飞行时间串联质谱法高通量分析美国红参的脑苷脂分子种

通讯作者: 徐杰

English

High throughput analysis of cerebroside molecular species from sea cucumber Parastichopus californicus by liquid chromatography-quadrupole-time-of-flight mass spectrometry

Corresponding author: 徐杰

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

液相色谱-四极杆-飞行时间串联质谱法高通量分析美国红参的脑苷脂分子种

通讯作者: 徐杰

English

High throughput analysis of cerebroside molecular species from sea cucumber Parastichopus californicus by liquid chromatography-quadrupole-time-of-flight mass spectrometry

Corresponding author: 徐杰

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs