基于氧化铈双重纳米酶活性的比率型比色传感器检测对氧磷

引用本文: 王翠, 白立鹏, 宋盼盼, 盖盼盼, 李峰. 基于氧化铈双重纳米酶活性的比率型比色传感器检测对氧磷[J]. 分析化学, 2023, 51(6): 954-961. doi: 10.19756/j.issn.0253-3820.231079 shu

Citation: WANG Cui, BAI Li-Peng, SONG Pan-Pan, GAI Pan-Pan, LI Feng. Ratiometric Colorimetry Sensor for Paraoxon Detection Based on Dual-Nanozyme Activity of Cerium Oxide[J]. Chinese Journal of Analytical Chemistry, 2023, 51(6): 954-961. doi: 10.19756/j.issn.0253-3820.231079 shu

基于氧化铈双重纳米酶活性的比率型比色传感器检测对氧磷

青岛农业大学化学与药学院, 青岛 266109

通讯作者: 盖盼盼,E-mail:ppgai@qau.edu.cn; 李峰,E-mail:lifeng@qau.edu.cn

基金项目:
国家自然科学基金项目(Nos.22274086,22076090)资助。

摘要: 基于氧化铈（CeO₂）的类有机磷水解酶和类氧化酶活性发展了一种比率型比色传感方法，实现了对氧磷的灵敏、准确检测。具有类有机磷水解酶活性的CeO₂纳米粒子（CeO₂ NPs）可催化对氧磷水解为对硝基苯酚，其在400 nm处有明显吸收峰，溶液体系由无色变为黄色； CeO₂还具有类氧化酶活性，催化3,3',5,5'-四甲基联苯胺（TMB）氧化为氧化态TMB，在653 nm处有明显吸收峰，溶液由无色变为蓝色。当目标物对氧磷存在时，400 nm处吸光度（A₄₀₀）增高；同时对氧磷抑制其类氧化酶活性，653 nm处吸光度（A₆₅₃）降低。基于对氧磷加入前后体系A₄₀₀/A₆₅₃比值的变化，实现了对氧磷的灵敏、准确检测，检出限为0.03 μmol/L（S/N=3）。利用本方法检测了韭菜样品中对氧磷的含量，加标回收率在92.0%~99.2%之间。本研究发展了一种基于CeO₂纳米酶双重活性的比率型比色传感检测方法，为食品中对氧磷残留的灵敏检测提供了新策略。

关键词:

English

Ratiometric Colorimetry Sensor for Paraoxon Detection Based on Dual-Nanozyme Activity of Cerium Oxide

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China

Corresponding author: GAI Pan-Pan, ; LI Feng,

Received Date: 03 March 2023
Revised Date: 23 March 2023
Fund Project:
Cerium oxide

Abstract: A ratiometric colorimetric sensor for sensitive detection of paraoxon was constructed based on the organophosphorus hydrolase-like activity and oxidase-like activity of cerium oxide nanoparticles (CeO₂ NPs). The CeO₂ NPs with OPH-like activity catalyzed hydrolysis of paraoxon to generate yellow p-nitrophenol, which had an obvious absorption peak at 400 nm. At the same time, CeO₂ NPs featured with oxidase-like activity could catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) into a blue oxidation state TMB, which had a significant absorption peak at 653 nm. In the presence of paraoxon, CeO₂ nanozyme hydrolyzed paraoxon to p-nitrophenol, and the absorbance value of the system at 400 nm (A₄₀₀) increased. Meanwhile, paraoxon inhibited the oxidase-like activity of CeO₂ nanozyme, and the absorbance value of the TMB system at 653 nm (A₆₅₃) decreased. The sensitive and accurate detection of paraoxon was realized based on the changes of A₄₀₀/A₆₅₃ before and after the addition of paraoxon, and the detection limit was 0.03 μmol/L (S/N=3). This method was used to detect the content of paraoxon in Chinese chives samples, and the recoveries were 92.0%-99.2%. This work developed a ratiometric colorimetric sensing method based on the dual-nanozyme activity of CeO₂ NPs, providing a new strategy for the sensitive detection of paraoxon residues in food.

Key words:

Supported by the National Natural Science Foundation of China (Nos. 22274086, 22076090).

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沈阳化工大学材料科学与工程学院沈阳 110142

基于氧化铈双重纳米酶活性的比率型比色传感器检测对氧磷

通讯作者: 盖盼盼,E-mail:ppgai@qau.edu.cn; 李峰,E-mail:lifeng@qau.edu.cn

English

Ratiometric Colorimetry Sensor for Paraoxon Detection Based on Dual-Nanozyme Activity of Cerium Oxide

Corresponding author: GAI Pan-Pan, ; LI Feng,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于氧化铈双重纳米酶活性的比率型比色传感器检测对氧磷

通讯作者: 盖盼盼,E-mail:ppgai@qau.edu.cn; 李峰,E-mail:lifeng@qau.edu.cn

English

Ratiometric Colorimetry Sensor for Paraoxon Detection Based on Dual-Nanozyme Activity of Cerium Oxide

Corresponding author: GAI Pan-Pan, ; LI Feng,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs