基于硝基修饰的锆基金属有机框架材料的分散固相萃取-高效液相色谱-串联质谱法测定食品中的米酵菌酸

引用本文: 方兰云, 江艳华, 李继革, 邱巧丽, 金米聪, 张丹丹, 张玉梅. 基于硝基修饰的锆基金属有机框架材料的分散固相萃取-高效液相色谱-串联质谱法测定食品中的米酵菌酸[J]. 分析化学, 2023, 51(6): 1024-1032. doi: 10.19756/j.issn.0253-3820.231040 shu

Citation: FANG Lan-Yun, JIANG Yan-Hua, LI Ji-Ge, QIU Qiao-Li, JIN Mi-Cong, ZHANG Dan-Dan, ZHANG Yu-Mei. Determination of Bongkrekic Acid in Food by Dispersive Solid Phase Extraction-High Performance Liquid Chromatography-Tandem Mass Spectrometry Using Nitro Modified Zirconium Metal Organic Framework[J]. Chinese Journal of Analytical Chemistry, 2023, 51(6): 1024-1032. doi: 10.19756/j.issn.0253-3820.231040 shu

基于硝基修饰的锆基金属有机框架材料的分散固相萃取-高效液相色谱-串联质谱法测定食品中的米酵菌酸

方兰云 ^1, ,
江艳华 ^2, ,
李继革 ^1, ,
邱巧丽 ^1, ,
金米聪 ^1, ,
张丹丹 ^1, ,
张玉梅 ^{3,
,}

1.
宁波市疾病预防控制中心, 浙江省微量有毒化学物健康风险评估技术研究重点实验室, 宁波 315010;
2.
中国科学院宁波材料技术与工程研究所, 宁波 315201;
3.
宁夏医科大学基础医学院, 银川 750004

通讯作者: 张玉梅,E-mail:23858956@qq.com

基金项目:
浙江省基础公益研究计划项目(No.LGC22H260002)、宁波市公益重点项目(No.2022S091)和宁夏自然科学基金项目(No.2022AAC03208)资助。

摘要: 建立了基于硝基修饰的锆基金属有机框架材料（NO₂-MOF）的分散固相萃取（DSPE）测定食品中米酵菌酸的方法。采用扫描电镜（SEM）、傅里叶变换红外光谱（FT-IR）、热重分析（TGA）、X射线衍射仪（XRD）、X射线光电子能谱（XPS）和氮气吸附实验等对合成的MOF材料进行了表征，结果表明制得了一种比表面积大、结构稳定的MOF材料。此材料与米酵菌酸分子之间存在静电作用、π-π堆积效应和氢键等分子间弱相互作用，基于此实现了对目标物米酵菌酸的选择性吸附。考察了此材料作为DSPE吸附剂吸附米酵菌酸的实验条件，包括溶液pH值、吸附剂分散方式、吸附剂用量、萃取时间以及解吸附溶剂类型等，结合高效液相色谱-串联质谱技术，建立了米酵菌酸的检测方法，方法的定量限为1.6 μg/kg，检出限为0.5 μg/kg。此方法前处理时间短、灵敏度高、对环境友好，木耳和米粉中米酵菌酸的加标回收率为75%~96%，准确度高，为相关食品中痕量米酵菌酸的测定提供了技术参考。

关键词:

English

Determination of Bongkrekic Acid in Food by Dispersive Solid Phase Extraction-High Performance Liquid Chromatography-Tandem Mass Spectrometry Using Nitro Modified Zirconium Metal Organic Framework

1.
Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China;
2.
Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China;
3.
School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China

Corresponding author: ZHANG Yu-Mei, 23858956@qq.com

Received Date: 10 February 2023
Revised Date: 07 April 2023
Fund Project:
Supported by the Basic Public Welfare Research Project of Zhejiang Province (No. LGC22H260002), the Ningbo Public Welfare Key Project (No. 2022S091) and the Ningxia Natural Science Foundation (No. 2022AAC03208).

Abstract: Dispersive solid phase extraction (DSPE) using nitro modified zirconium-based metal organic framework (NO₂-MOF) as adsorbent for determination of bongkrekic acid in food was developed. The synthesized NO₂-MOF were characterized by scanning electron microscopy (SEM), thermogravimetric analysis (TGA), infrared spectroscopy (IR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and nitrogen adsorption experiment, etc, indicating that a structural stable MOF material with large BET surface area was obtained. There existed electrostatic interaction, π-π stacking, hydrogen bond and other weak intermolecular interactions between MOF and bongkrekic acid molecules to achieve selective adsorption of target molecules. Main experimental factors affecting DSPE were optimized, including pH value, amount of the adsorbent, extraction time, and type of desorption solvent. The established method was applied to detection of bongkrekic acid in auricularia auricula and rice flour, with limit of quantification of 1.6 μg/kg, and limit of detection of 0.5 μg/kg. In conclusion, the established method for determination of bongkrekic acid in food by DSPE had the advantages such as short pretreatment time, high sensitivity and precision with satisfactory recoveries of 75%-96%, and was environmentally friendly. This work provided a more reliable technique for determination of extremely trace amount of bongkrekic acid in related food such as auricularia auricula, tremella and rice flour.

Key words:

Nitro modified
/ Zirconium metal organic framework
/ Dispersive solid phase extraction
/ High performance liquid chromatography-mass spectrometry
/ Bongkrekic acid
/ Food

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于硝基修饰的锆基金属有机框架材料的分散固相萃取-高效液相色谱-串联质谱法测定食品中的米酵菌酸

通讯作者: 张玉梅,E-mail:23858956@qq.com

English

Determination of Bongkrekic Acid in Food by Dispersive Solid Phase Extraction-High Performance Liquid Chromatography-Tandem Mass Spectrometry Using Nitro Modified Zirconium Metal Organic Framework

Corresponding author: ZHANG Yu-Mei, 23858956@qq.com

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于硝基修饰的锆基金属有机框架材料的分散固相萃取-高效液相色谱-串联质谱法测定食品中的米酵菌酸

通讯作者: 张玉梅,E-mail:23858956@qq.com

English

Determination of Bongkrekic Acid in Food by Dispersive Solid Phase Extraction-High Performance Liquid Chromatography-Tandem Mass Spectrometry Using Nitro Modified Zirconium Metal Organic Framework

Corresponding author: ZHANG Yu-Mei, 23858956@qq.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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