基于流体动力单细胞高效捕获的微流控芯片结构研究进展

引用本文: 潘婷, 武园园, 郭广生, 汪夏燕. 基于流体动力单细胞高效捕获的微流控芯片结构研究进展[J]. 分析化学, 2023, 51(6): 934-944. doi: 10.19756/j.issn.0253-3820.231008 shu

Citation: PAN Ting, WU Yuan-Yuan, GUO Guang-Sheng, WANG Xia-Yan. Advances in Microfluidic Chip Structures Based on Hydrodynamics of Efficient Single-Cell Capture[J]. Chinese Journal of Analytical Chemistry, 2023, 51(6): 934-944. doi: 10.19756/j.issn.0253-3820.231008 shu

基于流体动力单细胞高效捕获的微流控芯片结构研究进展

潘婷 ^1, ,
武园园 ^{1,
,} ,
郭广生 ^1,2, ,
汪夏燕 ^{1,
,}

1.
北京工业大学化学系, 北京 100124;
2.
中央民族大学国家安全研究院, 北京 100081

通讯作者: 武园园,E-mail:yuanyuanwu@bjut.edu.cn; 汪夏燕,E-mail:xiayanwang@bjut.edu.cn

基金项目:
国家自然科学基金项目(Nos.22127805,22176230)、北京高校卓越青年科学家计划项目(No.BJJWZYJH01201910005017)和中国博士后科学基金项目(No.2022M710277)资助。

摘要: 单细胞分析对于重大疾病的早期诊断及治疗、药物筛选和生理病理过程的研究具有重要意义。微流控芯片能够精确控制单细胞的微环境，实时监测单细胞的行为，已成为单细胞分析的强大工具。单细胞捕获是单细胞分析的重要步骤。目前已报道了多种微流控芯片用于单细胞捕获的方法，其中基于流体动力的微流控芯片单细胞捕获方法具有操作方便、单细胞捕获效率高等优点，受到研究人员的广泛关注及使用。为了全面了解基于流体动力的微流控芯片单细胞捕获方法的研究现状，掌握单细胞高效捕获的微流控芯片结构设计，实现单细胞精准快速分析，本文综述了基于流体动力的单细胞高效捕获（>70%）原理及微流控芯片结构，根据结构设计不同分为微井结构、微柱结构和旁路通道结构，介绍了单细胞高效捕获的微流控芯片优化过程，总结了微流控芯片的材质、结构特点及单细胞捕获效率等，对不同单细胞捕获结构的优势及不足进行了分析。最后，对基于流体动力的微流控芯片单细胞捕获方法的发展趋势进行了展望。

关键词:

English

Advances in Microfluidic Chip Structures Based on Hydrodynamics of Efficient Single-Cell Capture

1.
Department of Chemistry, Beijing University of Technology, Beijing 100124, China;
2.
Institute of National Security, Minzu University of China, Beijing 100081, China

Corresponding author: WU Yuan-Yuan, ; WANG Xia-Yan,

Received Date: 08 January 2023
Revised Date: 22 February 2023
Fund Project:
Supported by the National Natural Science Foundation of China (Nos. 22127805, 22176230), the Beijing Outstanding Young Scientist Program (No. BJJWZYJH01201910005017) and the China Postdoctoral Science Foundation (No. 2022M710277).

Abstract: Single-cell analysis is important for early diagnosis and treatment of major diseases, drug screening, and studying physiopathological processes. Microfluidic chips are capable of precisely controlling the microenvironment of single cells and monitoring their behavior in real-time, and have become a powerful tool for single-cell analysis. Single-cell capture is an important step in single-cell analysis. Till now, several microfluidic-chip-based single-cell capture methods have been reported, among which hydrodynamic microfluidicchip-based single-cell capture has the advantages such as easy operation and high-efficiency of single-cell capture, and thus has received wide attention and has been used by researchers. To comprehensively understand the research status of hydrodynamic microfluidic-chip-based single-cell capture, master the structural design of highefficiency single-cell capture microfluidic chips, and realize the accurate and rapid analysis of single cells, in this paper, the principle of efficient single-cell capture based on hydrodynamics and the structure of microfluidic chips are reviewed. There are three types of structures according to the structural design including micro-well structures, microcolumn structures and bypass channel structures. The optimization process of single-cell capture microfluidic chips is introduced. The materials, structural features, and single-cell capture efficiency of microfluidic chips are summarized, and the advantages and shortcomings of each single-cell capture structure are analyzed. Finally, the development trend of the hydrodynamic-based microfluidic chip single-cell capture method is prospected.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于流体动力单细胞高效捕获的微流控芯片结构研究进展

通讯作者: 武园园,E-mail:yuanyuanwu@bjut.edu.cn; 汪夏燕,E-mail:xiayanwang@bjut.edu.cn

English

Advances in Microfluidic Chip Structures Based on Hydrodynamics of Efficient Single-Cell Capture

Corresponding author: WU Yuan-Yuan, ; WANG Xia-Yan,

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通讯作者: 陈斌, bchen63@163.com

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English

Advances in Microfluidic Chip Structures Based on Hydrodynamics of Efficient Single-Cell Capture

Corresponding author: WU Yuan-Yuan, ; WANG Xia-Yan,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs