核酸酶驱动DNA步行器的构建及在食品安全检测中的应用

引用本文: 邓碧桃, 徐斐, 曹慧, 袁敏, 于劲松, 阴凤琴, 吴秀秀, 叶泰. 核酸酶驱动DNA步行器的构建及在食品安全检测中的应用[J]. 分析化学, 2023, 51(6): 911-921. doi: 10.19756/j.issn.0253-3820.221589 shu

Citation: DENG Bi-Tao, XU Fei, CAO Hui, YUAN Min, YU Jin-Song, YIN Feng-Qin, WU Xiu-Xiu, YE Tai. Construction of Nuclease-Powered DNA Walker and Its Application in Food Safety[J]. Chinese Journal of Analytical Chemistry, 2023, 51(6): 911-921. doi: 10.19756/j.issn.0253-3820.221589 shu

核酸酶驱动DNA步行器的构建及在食品安全检测中的应用

上海理工大学健康科学与工程学院, 上海食品快速检测工程技术研究中心, 上海 200093

通讯作者: 叶泰,E-mail:taiye@usst.edu.cn

基金项目:
国家自然科学基金项目(No.32172303)和上海市青年科技启明星计划项目(No.22QA1406500)资助。

摘要: 对食品中污染物的快速、高灵敏检测是预防食品安全事件发生的重要手段。DNA步行器（DNA walker）是一种在结构与功能上具有可编程性的动态纳米机器，其在自由能驱动下沿着预设轨道界面产生持续性机械位移。核酸酶驱动DNA步行器具有优异的信号放大能力和反应动力学，将其与核酸适配体相结合构建的生物传感器已被广泛应用于食品安全快速检测中。本文综述了核酸酶驱动的DNA步行器的构建及其在食品安全检测中的应用进展，并对其发展前景进行了展望。

关键词:

English

Construction of Nuclease-Powered DNA Walker and Its Application in Food Safety

Shanghai Engineering Research Center of Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China

Corresponding author: YE Tai, taiye@usst.edu.cn

Received Date: 30 November 2022
Revised Date: 04 March 2023
Fund Project:
Supported by the National Natural Science Foundation of China (No. 32172303) and the Shanghai Rising-Star Program (No. 22QA1406500).

Abstract: The rapid and highly sensitive detection of contaminants in foods is an important strategy to prevent food safety incidents. DNA Walker is a dynamic nano-machine with a programmable structure and function. It can be moved along the designed track interface driven by free energy to carry out continuous mechanical displacement. The nuclease powered DNA walker has excellent signal amplification capabilities and reaction kinetics, and the DNA walker-based biosensor combined with aptamer has been widely used in food safety detection. This review summarized the construction strategies of nuclease powered DNA walker and its application in food safety testing, and its further prospects were also discussed.

Key words:

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

核酸酶驱动DNA步行器的构建及在食品安全检测中的应用

通讯作者: 叶泰,E-mail:taiye@usst.edu.cn

English

Construction of Nuclease-Powered DNA Walker and Its Application in Food Safety

Corresponding author: YE Tai, taiye@usst.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

核酸酶驱动DNA步行器的构建及在食品安全检测中的应用

通讯作者: 叶泰,E-mail:taiye@usst.edu.cn

English

Construction of Nuclease-Powered DNA Walker and Its Application in Food Safety

Corresponding author: YE Tai, taiye@usst.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs