制备金纳米棒标记的免疫层析试纸条用于临床血清样本中心肌肌钙蛋白I的快速检测

引用本文: 王皓冬, 姚超群, 樊吉文, 贺玉泉, 王振新. 制备金纳米棒标记的免疫层析试纸条用于临床血清样本中心肌肌钙蛋白I的快速检测[J]. 分析化学, 2023, 51(6): 945-953. doi: 10.19756/j.issn.0253-3820.221536 shu

Citation: WANG Hao-Dong, YAO Chao-Qun, FAN Ji-Wen, HE Yu-Quan, WANG Zhen-Xin. Gold Nanorods-based Immunochromatographic Test Strip for Rapid Detection of Cardiac Troponin I in Clinical Serum Samples[J]. Chinese Journal of Analytical Chemistry, 2023, 51(6): 945-953. doi: 10.19756/j.issn.0253-3820.221536 shu

制备金纳米棒标记的免疫层析试纸条用于临床血清样本中心肌肌钙蛋白I的快速检测

王皓冬 ^1, ,
姚超群 ^2,3, ,
樊吉文 ^1, ,
贺玉泉 ^{1,
,} ,
王振新 ^{2,3,
,}

1.
吉林大学中日联谊医院心内科, 长春 130033;
2.
中国科学院长春应用化学研究所, 电分析化学国家重点实验室, 长春 130022;
3.
中国科学技术大学应用化学与工程学院, 合肥 230026

通讯作者: 贺玉泉,E-mail:heyq@jlu.edu.cn; 王振新,E-mail:wangzx@ciac.ac.cn

基金项目:
国家自然科学基金项目(No.21775145)资助。

摘要: 心肌肌钙蛋白I（Cardiac troponin I，cTnI）是心肌损伤的生物标志物之一，快速检测其血清水平对急性心肌梗死（Acute myocardial infarction，AMI）的临床诊断至关重要。本研究以鼠抗cTnI单克隆抗体（4T21cc-19C7cc，dAb）修饰的金纳米棒（Gold nanorod，GNR）为标记探针，采用双抗体夹心法制备侧流免疫层析试纸条（Lateral flow immunochromatographic test strip，LFITS），用于快速检测临床血清样本中的cTnI。此GNR标记的LFITS（GNR-LFITS）具有可定量检测、灵敏度高和特异性强的优点，检测血清中cTnI的线性范围为5~100 ng/mL，检出限（Limit of detection，LOD）为1.2 ng/mL，与其它蛋白的交叉反应值均小于5%。GNR-LFITS具有较高的实际应用能力，对临床血清样本中cTnI的检测结果与商用酶联免疫（Enzyme-linkedimmunosorbent assay，ELISA）检测试剂盒具有良好的相关性（R²=0.989），在AMI早期诊断中具有良好的应用前景。

关键词:

English

Gold Nanorods-based Immunochromatographic Test Strip for Rapid Detection of Cardiac Troponin I in Clinical Serum Samples

1.
Department of Cardiovascular, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
3.
School of Applied Chemical Engineering, University of Science and Technology of China, Hefei 230026, China

Corresponding author: HE Yu-Quan, ; WANG Zhen-Xin,

Received Date: 01 November 2022
Revised Date: 03 December 2022
Fund Project:
Supported by the National Natural Science Foundation of China (No.21775145).

Abstract: Cardiac troponin I (cTnI) is one of the standard biomarkers of myocardial injury, and the rapid detection of its concentration in serum is crucial for the diagnosis of acute myocardial infarction (AMI). In this study, gold nanorods (GNRs) modified with mouse anti-cTnI monoclonal antibody (4T21cc-19C7cc, dAb) were used as labeling probes to prepare lateral flow immunochromatographic test strips (LFITS) by double-antibody sandwich method for rapid detection of cTnI in clinical serum samples. The GNR-labeled LFITS (termed as GNRLFITS) showed several advantages including quantitative detection, high sensitivity and good specificity. The GNR-LFITS exhibited a linearity with the concentration of cTnI in the range of 5-100 ng/mL in serum, with a low limit of detection (LOD, 1.2 ng/mL) and low values of cross reaction with other protein interfering substances (<5%). Furthermore, the practicability of as-developed GNR-LFITS was demonstrated by quantitative determination of cTnI in clinical serum samples. The results of GNR-LFITS exhibited a good correlation with those of commercial enzyme-linked immunosorbent assay (ELISA) detection kit (R²=0.989), suggesting that GNR-LFITS had great potential in the early diagnosis of AMI.

Key words:

1. [1]
  THYGESEN K, ALPERT J S, JAFFE A S, CHAITMAN B R, BAX J J, MORROW D A, WHITE H D. Circulation, 2018, 138(20):e618-e651.THYGESEN K, ALPERT J S, JAFFE A S, CHAITMAN B R, BAX J J, MORROW D A, WHITE H D. Circulation, 2018, 138(20):e618-e651.
2. [2]
  GULATI M, LEVY P D, MUKHERJEE D, AMSTERDAM E, BHATT D L, BIRTCHER K K, BLANKSTEIN R, BOYD J, BULLOCK-PALMER R P, CONEJO T, DIERCKS D B, GENTILE F, GREENWOOD J P, HESS E P, HOLLENBERG S M, JABER W A, JNEID H, JOGLAR J A, MORROW D A, O'CONNOR R E, ROSS M A, SHAW L J. Circulation, 2021, 144(22):e368-e454.GULATI M, LEVY P D, MUKHERJEE D, AMSTERDAM E, BHATT D L, BIRTCHER K K, BLANKSTEIN R, BOYD J, BULLOCK-PALMER R P, CONEJO T, DIERCKS D B, GENTILE F, GREENWOOD J P, HESS E P, HOLLENBERG S M, JABER W A, JNEID H, JOGLAR J A, MORROW D A, O'CONNOR R E, ROSS M A, SHAW L J. Circulation, 2021, 144(22):e368-e454.
3. [3]
  AAKRE K M, SAENGER A K, BODY R, COLLINSON P, HAMMARSTEN O, JAFFE A S, KAVSAK P, OMLAND T, ORDONEZ-LIANOS J, APPLE F S. Clin. Chem., 2022, 68(8):1022-1030.AAKRE K M, SAENGER A K, BODY R, COLLINSON P, HAMMARSTEN O, JAFFE A S, KAVSAK P, OMLAND T, ORDONEZ-LIANOS J, APPLE F S. Clin. Chem., 2022, 68(8):1022-1030.
4. [4]
  MENDIS S, THYGESEN K, KUULASMAA K, GIAMPAOLI S, MAHONEN M, NGU BLACKETT K, LISHENG L. Int. J. Epidemiol., 2011, 40(1):139-146.MENDIS S, THYGESEN K, KUULASMAA K, GIAMPAOLI S, MAHONEN M, NGU BLACKETT K, LISHENG L. Int. J. Epidemiol., 2011, 40(1):139-146.
5. [5]
  RABER I, MCCARTHY C P, JANUZZI JR. J L. J. Am. Coll. Cardiol., 2021, 77(10):1357-1367.RABER I, MCCARTHY C P, JANUZZI JR. J L. J. Am. Coll. Cardiol., 2021, 77(10):1357-1367.
6. [6]
  TAJBAKHSH A, GHEIBI HAYAT S M, TAGHIZADEH H, AKBARI A, INABADI M, SAVARDASHTAKI A, JOHNSTON T P, SAHEBKAR A. Expert Rev. Anti-infective Ther., 2021, 19(3):345-357.TAJBAKHSH A, GHEIBI HAYAT S M, TAGHIZADEH H, AKBARI A, INABADI M, SAVARDASHTAKI A, JOHNSTON T P, SAHEBKAR A. Expert Rev. Anti-infective Ther., 2021, 19(3):345-357.
7. [7]
  CUMMINS B, AUCKLAND M L, CUMMINS P. Am. Heart J., 1987, 113(6):1333-1344.CUMMINS B, AUCKLAND M L, CUMMINS P. Am. Heart J., 1987, 113(6):1333-1344.
8. [8]
  CONRAD M J, JAROLIM P. Clin. Lab. Med., 2014, 34(1):59-73.CONRAD M J, JAROLIM P. Clin. Lab. Med., 2014, 34(1):59-73.
9. [9]
  HAN G R, KOO H J, KI H, KIM M G. ACS Appl. Mater. Interfaces, 2020, 12(31):34564-34575.HAN G R, KOO H J, KI H, KIM M G. ACS Appl. Mater. Interfaces, 2020, 12(31):34564-34575.
10. [10]
  WANG Z, GUO L, LIU L, KUANG H, XU C. Food Chem., 2018, 259:122-129.WANG Z, GUO L, LIU L, KUANG H, XU C. Food Chem., 2018, 259:122-129.
11. [11]
  ZHANG A, GAO T, WU S, YOU Z, ZHEN J, WAN F. Biochem. Biophys. Res. Commun., 2018, 507(1-4):110-113.ZHANG A, GAO T, WU S, YOU Z, ZHEN J, WAN F. Biochem. Biophys. Res. Commun., 2018, 507(1-4):110-113.
12. [12]
  XU R, FENG J, HONG Y, LV C, ZHAO D, LIN J, LU K, LI H, LIU J, CAO X, WANG T, ZAI J, WANG Z, JIA B, HAN Q, ZHU C. Infect. Dis. Poverty, 2017, 6:84.XU R, FENG J, HONG Y, LV C, ZHAO D, LIN J, LU K, LI H, LIU J, CAO X, WANG T, ZAI J, WANG Z, JIA B, HAN Q, ZHU C. Infect. Dis. Poverty, 2017, 6:84.
13. [13]
  ZHANG Hua, LI Qun, LIU Gui-Feng. Chin. J. Anal. Chem., 2020, 48(8):1018-1024. 张婳, 李群, 刘桂锋. 分析化学, 2020, 48(8):1018-1024.
14. [14]
  WANG J J, LIU B H, HSU Y T, YU F Y. Food Control, 2011, 22(6):964-969.WANG J J, LIU B H, HSU Y T, YU F Y. Food Control, 2011, 22(6):964-969.
15. [15]
  SHARMA A, TOK A I Y, ALAGAPPAN P, LIEDBERG B. Sens. Actuators, B, 2020, 312:127959.SHARMA A, TOK A I Y, ALAGAPPAN P, LIEDBERG B. Sens. Actuators, B, 2020, 312:127959.
16. [16]
  RYU Y, JIN Z, KANG M S, KIM H S. BioChip J., 2011, 5(3):193-198.RYU Y, JIN Z, KANG M S, KIM H S. BioChip J., 2011, 5(3):193-198.
17. [17]
  SONG Y, CAI X, OSTERMEYER G, YU J, DU D, LIN Y. ACS Sens., 2021, 6(1):220-228.SONG Y, CAI X, OSTERMEYER G, YU J, DU D, LIN Y. ACS Sens., 2021, 6(1):220-228.
18. [18]
  ZHANG D, HUANG L, LIU B, NI H, SUN L, SU E, CHEN H, GU Z, ZHAO X. Biosens. Bioelectron., 2018, 106:204-211.ZHANG D, HUANG L, LIU B, NI H, SUN L, SU E, CHEN H, GU Z, ZHAO X. Biosens. Bioelectron., 2018, 106:204-211.
19. [19]
  HUANG L, TIAN S, ZHAO W, LIU K, MA X, GUO J, YIN M. Analyst, 2021, 146(24):7702-7709.HUANG L, TIAN S, ZHAO W, LIU K, MA X, GUO J, YIN M. Analyst, 2021, 146(24):7702-7709.
20. [20]
  ZHENG J, CHENG X, ZHANG H, BAI X, AI R, SHAO L, WANG J. Chem. Rev., 2021, 121(21):13342-13453.ZHENG J, CHENG X, ZHANG H, BAI X, AI R, SHAO L, WANG J. Chem. Rev., 2021, 121(21):13342-13453.
21. [21]
  ZHENG X J, QIU J D, ZHANG L, WANG Z X, LIANG R P. Chem. Commun., 2013, 49(34):3546-3548.ZHENG X J, QIU J D, ZHANG L, WANG Z X, LIANG R P. Chem. Commun., 2013, 49(34):3546-3548.
22. [22]
  LIN T, WU Y, LI Z, SONG Z, GUO L, FU F. Anal. Chem., 2016, 88(22):11022-11027.LIN T, WU Y, LI Z, SONG Z, GUO L, FU F. Anal. Chem., 2016, 88(22):11022-11027.
23. [23]
  SAA L, CORONADO-PUCHAU M, PAVLOV V, LIZ-MARZÁN L M. Nanoscale, 2014, 6(13):7405-7409.SAA L, CORONADO-PUCHAU M, PAVLOV V, LIZ-MARZÁN L M. Nanoscale, 2014, 6(13):7405-7409.
24. [24]
  RAJ V, ALEX S. Gold Bull., 2021, 54(1):1-7.RAJ V, ALEX S. Gold Bull., 2021, 54(1):1-7.
25. [25]
  LEE K W, KIM K R, CHUN H J, JEONG K Y, HONG D K, LEE K N, YOON H C. Biosens. Bioelectron., 2020, 163:112284.LEE K W, KIM K R, CHUN H J, JEONG K Y, HONG D K, LEE K N, YOON H C. Biosens. Bioelectron., 2020, 163:112284.
26. [26]
  TANG L, CASAS J. Biosens. Bioelectron., 2014, 61:70-75.TANG L, CASAS J. Biosens. Bioelectron., 2014, 61:70-75.
27. [27]
  TANG L, CASAS J, VENKATARAMASUBRAMANI M. Anal. Chem., 2013, 85(3):1431-1439.TANG L, CASAS J, VENKATARAMASUBRAMANI M. Anal. Chem., 2013, 85(3):1431-1439.
28. [28]
  XU Feng-Qin, ZHU Qun-Yan, ZHANG Hua, WANG Zhen-Xin. Chin. J. Anal. Chem., 2021, 49(6):973-981. 徐凤琴, 朱群艳, 张婳, 王振新. 分析化学, 2021, 49(6):973-981.
29. [29]
  ARMBRUSTER D, PRY T. Clin. Biochem. Rev., 2008, 29:S49-S52.ARMBRUSTER D, PRY T. Clin. Biochem. Rev., 2008, 29:S49-S52.
30. [30]
  TARANOVA N A, SLOBODENUYK V D, ZHERDEV A V, DZANTIEV B B. RSC Adv., 2021, 11(27):16445-16452.TARANOVA N A, SLOBODENUYK V D, ZHERDEV A V, DZANTIEV B B. RSC Adv., 2021, 11(27):16445-16452.
31. [31]
  NATARAJAN S, JAYARAJ J, PRAZERES D M F. Biosensors, 2021, 11(2):49.NATARAJAN S, JAYARAJ J, PRAZERES D M F. Biosensors, 2021, 11(2):49.
32. [32]
  BYZOVA N A, ZHERDEV A V, KHLEBTSOV B N, BUROV A M, KHLEBTSOV N G, DZANTIEV B B. Sensors, 2020, 20(12):3608.BYZOVA N A, ZHERDEV A V, KHLEBTSOV B N, BUROV A M, KHLEBTSOV N G, DZANTIEV B B. Sensors, 2020, 20(12):3608.

扫一扫看文章

计量

PDF下载量: 13
文章访问数: 844
HTML全文浏览量: 66

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

制备金纳米棒标记的免疫层析试纸条用于临床血清样本中心肌肌钙蛋白I的快速检测

通讯作者: 贺玉泉,E-mail:heyq@jlu.edu.cn; 王振新,E-mail:wangzx@ciac.ac.cn

English

Gold Nanorods-based Immunochromatographic Test Strip for Rapid Detection of Cardiac Troponin I in Clinical Serum Samples

Corresponding author: HE Yu-Quan, ; WANG Zhen-Xin,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

制备金纳米棒标记的免疫层析试纸条用于临床血清样本中心肌肌钙蛋白I的快速检测

通讯作者: 贺玉泉,E-mail:heyq@jlu.edu.cn; 王振新,E-mail:wangzx@ciac.ac.cn

English

Gold Nanorods-based Immunochromatographic Test Strip for Rapid Detection of Cardiac Troponin I in Clinical Serum Samples

Corresponding author: HE Yu-Quan, ; WANG Zhen-Xin,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs