基于荧光体系的酶联免疫吸附分析法的研究进展

引用本文: 刘国永, 石宇, 孙健, 穆婧. 基于荧光体系的酶联免疫吸附分析法的研究进展[J]. 分析化学, 2023, 51(3): 331-339. doi: 10.19756/j.issn.0253-3820.221449 shu

Citation: LIU Guo-Yong, SHI Yu, SUN Jian, MU Jing. Recent Advances on Fluorescence-based Enzyme Linked Immunosorbent Assay[J]. Chinese Journal of Analytical Chemistry, 2023, 51(3): 331-339. doi: 10.19756/j.issn.0253-3820.221449 shu

基于荧光体系的酶联免疫吸附分析法的研究进展

刘国永 ^1, ,
石宇 ^1, ,
孙健 ^{2,
,} ,
穆婧 ^{1,
,}

1.
深圳北京大学香港科技大学医学中心, 北京大学深圳医院精准医学研究院, 深圳 518036;
2.
中国科学院长春应用化学研究所, 电分析化学国家重点实验室, 长春 130022

通讯作者: 孙健,E-mail:jiansun@ciac.ac.cn; 穆婧,E-mail:jingmu1921@163.com

基金项目:
国家自然科学基金项目(No.32101074)、吉林省科技发展计划项目(No.20200201091JC)和北京大学深圳医院科研启动项目(No.KYQD202100X)资助。

摘要: 酶联免疫吸附分析(Enzyme linked immunosorbent assay,ELISA)作为一种重要的免疫分析方法,具有高通量、信号读取方便、操作简单和成本低廉等优点,广泛应用于环境监测、食品安全检测以及医疗诊断等领域。然而,由于受比色显色原理的限制,传统ELISA的检测灵敏度难以提高。为了解决此问题,研究者开发了多种类型的新型检测体系。其中,荧光方法由于具有灵敏度高、操作简单以及响应快速等优势,引起了广泛关注。目前,多种新型荧光材料已被广泛用于构建ELISA,促进了ELISA在分析化学和生物医疗检测中的应用。本文详细介绍了有机小分子、硅/碳纳米粒子、金属纳米簇和量子点等荧光材料构建的ELISA,根据使用标记酶的不同,介绍了以碱性磷酸酶、辣根过氧化物酶等酶作为标记酶的ELISA,评述了近五年来荧光材料在构建ELISA方面的研究进展,并对其应用前景与发展趋势进行了展望。

关键词:

English

Recent Advances on Fluorescence-based Enzyme Linked Immunosorbent Assay

LIU Guo-Yong ^1, ,
SHI Yu ^1, ,
SUN Jian ^{2,
,} ,
MU Jing ^{1,
,}

1.
Institute of Precision Medicine, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen 518036, China;
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: SUN Jian, ; MU Jing,

Received Date: 14 September 2022
Revised Date: 29 January 2023
Fund Project:
Supported by the National Natural Science Foundation of China (No. 32101074), the Development Project of Science and Technology of Jilin Province, China (No. 20200201091JC) and the Scientific Research Foundation of Peking University Shenzhen Hospital (KYQD202100X).

Abstract: Due to advantages in high-throughput, easy reading, simple operation and low cost, enzyme linked immunosorbent assay (ELISA) plays an important role in environmental monitoring, food safety testing and medical diagnosis. However, the insufficient chromogenic part of ELISA results in high detection limit, which limits the further application of ELISA in analysis and detection. To overcome this issue, the conventional ELISA has been improved by many methods. Among them, the fluorometric method has attracted widespread attention given its high sensitivity, simple operation, and fast response. Recently, a variety of fluorescent materials have been developed to construct different types of ELISA, which promotes the application of ELISA in analytical chemistry and biomedical detection. In this paper, the ELISA constructed by fluorescent materials such as organic small molecules, silicon/carbon nanoparticles, metal nanoclusters, and quantum dots was introduced in detail. Additionally, different enzymes in ELISA, including alkaline phosphatase, horseradish peroxidase and other enzyme as labeling enzymes, were systematically reviewed. Furthermore, the prospects of ELISA based on different fluorescent materials were also discussed.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于荧光体系的酶联免疫吸附分析法的研究进展

通讯作者: 孙健,E-mail:jiansun@ciac.ac.cn; 穆婧,E-mail:jingmu1921@163.com

English

Recent Advances on Fluorescence-based Enzyme Linked Immunosorbent Assay

Corresponding author: SUN Jian, ; MU Jing,

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中国化学会秘书处

基于荧光体系的酶联免疫吸附分析法的研究进展

通讯作者: 孙健,E-mail:jiansun@ciac.ac.cn; 穆婧,E-mail:jingmu1921@163.com

English

Recent Advances on Fluorescence-based Enzyme Linked Immunosorbent Assay

Corresponding author: SUN Jian, ; MU Jing,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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