生物矿化法合成乳铁蛋白硫化铜纳米粒子及其光热抗肿瘤性能研究

引用本文: 范博, 刘岚, 吴倩, 郑子良, 邢洋, 张娟, 张瑞平. 生物矿化法合成乳铁蛋白硫化铜纳米粒子及其光热抗肿瘤性能研究[J]. 分析化学, 2023, 51(2): 239-249. doi: 10.19756/j.issn.0253-3820.221293 shu

Citation: FAN Bo, LIU Lan, WU Qian, ZHENG Zi-Liang, XING Yang, ZHANG Juan, ZHANG Rui-Ping. Biomineralization-inspired Synthesis of Lactoferrin-mediated Copper Sulfide Nanoparticle and Its Photothermal Ablation for Tumor Therapy[J]. Chinese Journal of Analytical Chemistry, 2023, 51(2): 239-249. doi: 10.19756/j.issn.0253-3820.221293 shu

生物矿化法合成乳铁蛋白硫化铜纳米粒子及其光热抗肿瘤性能研究

范博 ^1,2, ,
刘岚 ^1, ,
吴倩 ^1, ,
郑子良 ^2, ,
邢洋 ^1,2, ,
张娟 ^2, ,
张瑞平 ^{1,2,
,}

1.
山西医科大学药学院, 太原 030001;
2.
山西医科大学第三医院, 山西白求恩医院, 山西医学科学院, 太原 030032

通讯作者: 张瑞平,E-mail:zrp_7142@sxmu.edu.cn

基金项目:
国家自然科学基金项目(Nos.82211001138,82071987,81771907)、山西省青年科学研究项目(No.20210302124703)和山西医科大学博士启动基金项目(No.03201620)资助。

摘要: 基于近红外光二区（NIR-Ⅱ）的肿瘤光热治疗具有生物组织穿透更深、组织散射或吸收更少、皮肤最大允许暴露光照功率更高等优点。本研究采用生物矿化法一步合成了结构简单、生物相容性高、稳定性好且具有肿瘤靶向作用的乳铁蛋白硫化铜纳米粒子（Lactoferrin-mediated copper sulfide nanoparticles，CuS@Lf NPs）。此纳米粒子在NIR-II窗口具有光吸收，可在1064 nm激光照射下实现肿瘤光热治疗。乳铁蛋白不仅改善了硫化铜纳米粒子的溶解性，提高了其生物相容性和肿瘤靶向性，而且利用Lf的抗氧化作用可清除光热治疗后的氧化损伤。采用紫外-可见-近红外吸收光谱考察了CuS@Lf NPs的光吸收性质，结果表明，其在NIR-Ⅱ区1000~1300 nm范围内具有较强吸收，在1064 nm激光照射下实现了有效的光热转换，光热转化效率为25.84%，光热稳定性好。以LRP-1高表达的U87神经胶质瘤为模型，研究了CuS@Lf NPs的体内外抗肿瘤作用，结果表明，CuS@Lf NPs具有优良的体内光热性能，在NIR-Ⅱ区1064 nm激光照射下可有效实现肿瘤的热消融，安全性好，为实现NIR-Ⅱ光热抗肿瘤治疗在临床中的应用奠定了基础。

关键词:

English

Biomineralization-inspired Synthesis of Lactoferrin-mediated Copper Sulfide Nanoparticle and Its Photothermal Ablation for Tumor Therapy

1.
School of Pharmacy, Shanxi Medical University, Taiyuan 030001, China;
2.
Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Taiyuan 030032, China

Corresponding author: ZHANG Rui-Ping, zrp_7142@sxmu.edu.cn

Received Date: 04 June 2022
Revised Date: 14 October 2022
Fund Project:
Supported by the National Natural Science Foundation of China (Nos. 82211001138, 82071987, 81771907), the Youth Scientific Research Project of Shanxi Province (No. 20210302124703) and the Foundation for PhD of Shanxi Medical University (No 03201620).

Abstract: Photothermal agents with strong light absorption in the second near-infrared (NIR-II) region are strongly desired for successful photothermal therapy (PTT). In this work, the lactoferrin-mediated copper sulfide nanoparticles (CuS@Lf NPs) were successfully synthesized by biomineralization strategy. The uniform CuS@Lf NPs showed strong NIR-Ⅱ absorbance in the wavelength range of 1000-1300 nm and possessed high photothermal conversion efficiency, which could be applied in antitumor therapy under 1064 nm laser irradiation. Moreover, lactoferrin (Lf) not only improved the solubility of CuS nanoparticles, but also enhanced their biocompatibility and tumor targeting. Meanwhile, the antioxidant effect of Lf could eliminate oxidative damage after photothermal therapy and reduce tumor recurrence. Herein, the U87 glioma model with high expression of low density lipoprotein receptor associated protein (LRP-1) was used to study the antitumor effects of CuS@Lf NPs in vivo and in vitro. As a result, CuS@Lf NPs displayed effective photothermal tumor ablation, which provided a foundation for NIR-Ⅱ based photothermal therapy in antitumor application.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

生物矿化法合成乳铁蛋白硫化铜纳米粒子及其光热抗肿瘤性能研究

通讯作者: 张瑞平,E-mail:zrp_7142@sxmu.edu.cn

English

Biomineralization-inspired Synthesis of Lactoferrin-mediated Copper Sulfide Nanoparticle and Its Photothermal Ablation for Tumor Therapy

Corresponding author: ZHANG Rui-Ping, zrp_7142@sxmu.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

生物矿化法合成乳铁蛋白硫化铜纳米粒子及其光热抗肿瘤性能研究

通讯作者: 张瑞平,E-mail:zrp_7142@sxmu.edu.cn

English

Biomineralization-inspired Synthesis of Lactoferrin-mediated Copper Sulfide Nanoparticle and Its Photothermal Ablation for Tumor Therapy

Corresponding author: ZHANG Rui-Ping, zrp_7142@sxmu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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