基于生物阻抗谱的干细胞发育过程电学特性研究

引用本文: 刘凯, 程晓璇, 万建芬, 潘化平, 姚佳烽. 基于生物阻抗谱的干细胞发育过程电学特性研究[J]. 分析化学, 2022, 50(8): 1188-1195. doi: 10.19756/j.issn.0253-3820.221116 shu

Citation: LIU Kai, CHENG Xiao-Xuan, WAN Jian-Fen, PAN Hua-Ping, YAO Jia-Feng. Development of an Electrical Impedance Sensor for Monitoring Stem Cell Growth[J]. Chinese Journal of Analytical Chemistry, 2022, 50(8): 1188-1195. doi: 10.19756/j.issn.0253-3820.221116 shu

基于生物阻抗谱的干细胞发育过程电学特性研究

刘凯 ^1, ,
程晓璇 ^1, ,
万建芬 ^1, ,
潘化平 ^2,3, ,
姚佳烽 ^{1,3,
,}

1.
南京航空航天大学机电学院, 南京 210016;
2.
南京医科大学附属江宁医院, 南京 211199;
3.
医工融合联合实验室, 南京 211199

通讯作者: 姚佳烽,E-mail:jiaf.yao@nuaa.edu.cn

基金项目:
国家自然科学基金项目(No.62071224)、江苏省重点研发计划社会发展-面上项目(No. BE2021618)、精密测试技术及仪器国家重点实验室(天津大学)开放课题项目(No.pilab2107)和南京医科大学附属江宁医院医工融合实验室开放课题项目(No.JNYYZXKY202105)资助。

摘要: 基于生物阻抗谱方法研究了干细胞发育过程的电学特性,开发了一种用于干细胞发育过程监测的高精度生物阻抗谱传感器。此传感器采用倒锥形结构,实现了检测目标尺寸的自适应,通过渐缩腔室实现电场的聚集,通过窄缩腔室实现了检测区域处电场均匀化,提高了干细胞的检测精度。采用数值仿真方法对传感器的结构进行了验证及优化,结果表明,此传感器可避免干细胞发育过程中的位置变动对检测结果的影响,并且对发育中干细胞的尺寸变化更敏感,通过对传感器结构参数进行优化,使最优监测灵敏度达到0.882。以斑马鱼胚胎干细胞为监测对象,采用本方法对其发育过程进行了30 h监测,发现随着斑马鱼胚胎的发育,阻抗值逐渐增大,弛豫频率逐渐减小(由1870 kHz降低到481 kHz)。结果表明,本传感器与监测方法可实现干细胞发育过程的高精度监测。

关键词:

English

Development of an Electrical Impedance Sensor for Monitoring Stem Cell Growth

1.
School of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China;
2.
Jiangning Hospital Affiliated to Nanjing Medical University, Nanjing 211199, China;
3.
Joint Laboratory of Medical-Industrial Integration, Nanjing 211199, China

Corresponding author: YAO Jia-Feng, jiaf.yao@nuaa.edu.cn

Received Date: 09 March 2022
Revised Date: 25 April 2022
Fund Project:
Supported by the National Natural Science Foundation of China (No.62071224), the Jiangsu Provincial Key R&D Program Social Development-General Project (No.BE2021618), the Open Project of State Key Laboratory of Precision Testing Technology and Instruments (Tianjin University) (No.pilab2107), and the Medical Engineering of Jiangning Hospital Affiliated to Nanjing Medical University Fusion Laboratory Open Project (No.JNYYZXKY202105).

Abstract: The electrical properties of stem cells during development were investigated based on bioimpedance spectroscopy. First, a high-precision bioimpedance spectroscopy sensor for monitoring stem cell development process was developed. The sensor adopted an inverted tapered structure to realize the self-adaptation of the detection target size, and the electric field was concentrated and narrowed by the tapered chamber. The electric field in the detection area was homogenized to improve the detection accuracy of stem cells. Then, the structure of the sensor was verified and optimized by numerical simulation method. The results showed that the sensor could avoid the influence of positional changes during the development of stem cells on the detection results, and was more sensitive to the size changes of developing stem cells. The structural parameters were optimized, and the optimal monitoring sensitivity was 0.882. Finally, the proposed method was verified by experiment. Taking zebrafish embryonic stem cells as the monitoring object, the development process was monitored for 30 hours. The experimental results showed that with the development of zebrafish embryos, the impedance value gradually increased, and the relaxation frequency decreased gradually (from 1870 kHz to 481 kHz). The results showed that the sensor and monitoring method designed in this work could achieve high-precision monitoring of the developmental process of stem cells.

Key words:

1. [1]
  FU X, HE Q, TAO Y, WANG M, WANG W, WANG Y, YU Q, ZHANG F, ZHANG X, CHEN Y G, GAO D, HU P, HUI L, WANG X, ZENG Y A. Sci. China Life Sci., 2021, 64(12):1998-2029.FU X, HE Q, TAO Y, WANG M, WANG W, WANG Y, YU Q, ZHANG F, ZHANG X, CHEN Y G, GAO D, HU P, HUI L, WANG X, ZENG Y A. Sci. China Life Sci., 2021, 64(12):1998-2029.
2. [2]
  ZHAO Y, WANG M, LIANG F, LI J. Stem Cell Res. Ther., 2021, 12(1):588.ZHAO Y, WANG M, LIANG F, LI J. Stem Cell Res. Ther., 2021, 12(1):588.
3. [3]
  FORBESL H, ANDREWS M R. Neural Reneger. Res., 2021, 16(4):614-617.FORBESL H, ANDREWS M R. Neural Reneger. Res., 2021, 16(4):614-617.
4. [4]
  CHAN H J, YANSHRE E, ROY J, TIPOE G L, FUNG M L, LIM L W. Int. J. Mol. Sci., 2021, 22(18):10151.CHAN H J, YANSHRE E, ROY J, TIPOE G L, FUNG M L, LIM L W. Int. J. Mol. Sci., 2021, 22(18):10151.
5. [5]
  ITO E, IHA K, YOSHIMURA T, NAKAISHI K, WATABE S. Adv. Clin. Chem, 2021, 101:121-133.ITO E, IHA K, YOSHIMURA T, NAKAISHI K, WATABE S. Adv. Clin. Chem, 2021, 101:121-133.
6. [6]
  LIN Q, HUANG Z, YE X, YANG B, FANG X, LIU B, CHEN H, KONG J. Talanta, 2021, 225:122090.LIN Q, HUANG Z, YE X, YANG B, FANG X, LIU B, CHEN H, KONG J. Talanta, 2021, 225:122090.
7. [7]
  BRAUN A C, CAMPOS F A B, ABDALLAH E A, RUANO A P C, MEDINA T S, TARIKI M S, PINTO F F E, MELLO C A L, CHINEN L T D. Front. Oncol., 2021, 11:622626.BRAUN A C, CAMPOS F A B, ABDALLAH E A, RUANO A P C, MEDINA T S, TARIKI M S, PINTO F F E, MELLO C A L, CHINEN L T D. Front. Oncol., 2021, 11:622626.
8. [8]
  YAO Jia-Feng, WAN Jian-Fen, YANG Lu, LIU Kai, CHEN Bai, WU Hong-Tao. J. Acta Phys. Sin.,2020, 69(16):90-97.姚佳烽,万建芬,杨璐,刘凯,陈柏,吴洪涛.物理学报, 2020, 69(16):90-97.
9. [9]
  CHEN Chang-Guo, LI Lei-Guang, LIU Yu-Ping, FAN Yu-Jing. Chin. J. Anal. Chem., 2010, 38(6):855-858.陈昌国,李雷光,刘渝萍,范玉静.分析化学, 2010, 38(6):855-858.
10. [10]
  DETREZ E, KERZERHO V, BELHAJ M M, VERGNET A, VERDAL H, ROUYER T, BONHOMMEAU S, LAMLIH A, JULIEN M, BEN A F, RENOVELL M, BERNARD S, SOULIER F. Aquaculture, 2022, 547:737396.DETREZ E, KERZERHO V, BELHAJ M M, VERGNET A, VERDAL H, ROUYER T, BONHOMMEAU S, LAMLIH A, JULIEN M, BEN A F, RENOVELL M, BERNARD S, SOULIER F. Aquaculture, 2022, 547:737396.
11. [11]
  RAPOPORT Y, GRIMALSKY V, FEDUN V, AGAPITOV O, BONNELL J, GRYTSAI A, MILINEVSKY G, LIASHCHUK A, ROZHNOI A, SOLOVIEVA M, GULIN A. Ann. Geophys., 2020, 38(1):207-230.RAPOPORT Y, GRIMALSKY V, FEDUN V, AGAPITOV O, BONNELL J, GRYTSAI A, MILINEVSKY G, LIASHCHUK A, ROZHNOI A, SOLOVIEVA M, GULIN A. Ann. Geophys., 2020, 38(1):207-230.
12. [12]
  WANG L, HU S, LIU K, CHEN B, WU H, JIA J, YAO J. Rev. Sci. Instrum., 2020, 91(12):124104.WANG L, HU S, LIU K, CHEN B, WU H, JIA J, YAO J. Rev. Sci. Instrum., 2020, 91(12):124104.
13. [13]
  YAO Jia-Feng, HU Song-Pei, YANG Lu, WU Yang, HAN Wei, LIU Kai. Acta Phys. Sin., 2021, 70(15):358-364.姚佳烽,胡松佩,杨璐,吴阳,韩伟,刘凯.物理学报, 2021, 70(15):358-364.
14. [14]
  VEMBADI A, MENACHERY A, QASAIMEH M A. Front. Bioeng. Biotechnol., 2019, 7:147.VEMBADI A, MENACHERY A, QASAIMEH M A. Front. Bioeng. Biotechnol., 2019, 7:147.
15. [15]
  GAWAD S, SCHILD L, RENAUD P. Lab Chip, 2001, 1(1):76-82.GAWAD S, SCHILD L, RENAUD P. Lab Chip, 2001, 1(1):76-82.
16. [16]
  DE NINNO A, ERRICO V, BERTANI F R, BUSINARO L, BISEGNA P, CASELLI F. Lab Chip, 2017, 17(6):1158-1166.DE NINNO A, ERRICO V, BERTANI F R, BUSINARO L, BISEGNA P, CASELLI F. Lab Chip, 2017, 17(6):1158-1166.
17. [17]
  ASAMI K, IRIMAJIRI A. Phys. Med. Biol., 2000, 45(11):3285-3297.ASAMI K, IRIMAJIRI A. Phys. Med. Biol., 2000, 45(11):3285-3297.
18. [18]
  SONG J H, LEE S M, YOO K H. RSC Adv., 2018, 8(54):31246-31254.SONG J H, LEE S M, YOO K H. RSC Adv., 2018, 8(54):31246-31254.
19. [19]
  ZHANG Z Z, ZHENG T Y, ZHU R. Anal. Chem., 2020, 92(18):12579-12587.ZHANG Z Z, ZHENG T Y, ZHU R. Anal. Chem., 2020, 92(18):12579-12587.
20. [20]
  HUA S Z, PENNELL T. Lab Chip, 2009, 9(2):251-256.HUA S Z, PENNELL T. Lab Chip, 2009, 9(2):251-256.
21. [21]
  LEI K F, HO Y C, HUANG C H, HUANG C H, PAI P C. Talanta, 2021, 229:122259.LEI K F, HO Y C, HUANG C H, HUANG C H, PAI P C. Talanta, 2021, 229:122259.
22. [22]
  AMERI S K, SINGH P K, DOKMECI M R, KHADEMHOSSEINI A, XU Q, SONKUSALE S R. Biosens. Bioelectron., 2014, 54:462-467.AMERI S K, SINGH P K, DOKMECI M R, KHADEMHOSSEINI A, XU Q, SONKUSALE S R. Biosens. Bioelectron., 2014, 54:462-467.
23. [23]
  ZHANG F, JIN T, HU Q, HE P. J. Electroanal. Chem., 2018, 823:531-536.ZHANG F, JIN T, HU Q, HE P. J. Electroanal. Chem., 2018, 823:531-536.
24. [24]
  WAN J, YIN H, LIU K, ZHU C, GUAN X, YAO J. IEEE Sens. J., 2021, 1(1):1-9.WAN J, YIN H, LIU K, ZHU C, GUAN X, YAO J. IEEE Sens. J., 2021, 1(1):1-9.
25. [25]
  SUN T, GREEN N G, MORGAN H. Nano, 2008, 3(1):55-63.SUN T, GREEN N G, MORGAN H. Nano, 2008, 3(1):55-63.

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于生物阻抗谱的干细胞发育过程电学特性研究

通讯作者: 姚佳烽,E-mail:jiaf.yao@nuaa.edu.cn

English

Development of an Electrical Impedance Sensor for Monitoring Stem Cell Growth

Corresponding author: YAO Jia-Feng, jiaf.yao@nuaa.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于生物阻抗谱的干细胞发育过程电学特性研究

通讯作者: 姚佳烽,E-mail:jiaf.yao@nuaa.edu.cn

English

Development of an Electrical Impedance Sensor for Monitoring Stem Cell Growth

Corresponding author: YAO Jia-Feng, jiaf.yao@nuaa.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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