基于石墨烯量子点的“Turn-on”型荧光探针用于Fe的选择性检测

引用本文: 季茂菁, 海欣, 周璐, 刘安楠, 崔竹梅, 毕赛. 基于石墨烯量子点的“Turn-on”型荧光探针用于Fe的选择性检测[J]. 分析化学, 2022, 50(7): 1005-1013. doi: 10.19756/j.issn.0253-3820.221076 shu

Citation: JI Mao-Jing, HAI Xin, ZHOU Lu, LIU An-Nan, CUI Zhu-Mei, BI Sai. raphene Quantum Dots-based Fluorescence “Turn-On” Probe for Selective Detection of Fe(Ⅱ)[J]. Chinese Journal of Analytical Chemistry, 2022, 50(7): 1005-1013. doi: 10.19756/j.issn.0253-3820.221076 shu

基于石墨烯量子点的“Turn-on”型荧光探针用于Fe的选择性检测

季茂菁 ^1, ,
海欣 ^2, ,
周璐 ^2, ,
刘安楠 ^2, ,
崔竹梅 ^{1,
,} ,
毕赛 ^{1,2,
,}

1.
青岛大学附属医院妇科, 青岛 266003;
2.
青岛大学化学化工学院, 青岛 266071

通讯作者: 崔竹梅,E-mail:cuizhumei1966@126.com; 毕赛,E-mail:bisai11@126.com

基金项目:
国家自然科学基金项目(Nos.22076087,22004077,81802923)和山东省自然科学基金项目(Nos.ZR2020JQ08,ZR2019MH121)资助。

摘要: 开发了一种基于对苯二甲酸功能化石墨烯量子点的"Turn-on "型荧光探针,用于选择性检测Fe²⁺。以氧化石墨烯为碳源,对苯二甲酸为修饰基团,氢氧化钾为切割剂、过氧化氢为辅助切割剂,采用一步水热法制备得到对苯二甲酸功能化石墨烯量子点(TPA@GQDs)。采用多种表征手段研究了TPA@GQDs的结构与组成,深入探索了TPA@GQDs的光学性质及其作为荧光探针的可行性。基于Fe²⁺的推电子作用引发TPA@GQDs荧光增强,构建了" Turn-on"型荧光探针用于Fe²⁺的灵敏检测,Fe²⁺浓度在0.33~20 μmol/L和20~60 μmol/L两段范围内分别与F/F₀具有良好的线性关系,线性方程分别为F/F₀=0.0638C+1.1385(R²=0.9974)和F/F₀=0.0244C+1.9215(R²=0.9989),检出限为0.33 μmol/L (3σ)。此荧光检测体系具有良好的选择性,Fe³⁺对检测基本无影响。将此探针应用于地下水中Fe²⁺的测定,回收率为98.5%~102.0%,表明此荧光探针在水质监测中具有广阔的应用前景。

关键词:

English

raphene Quantum Dots-based Fluorescence “Turn-On” Probe for Selective Detection of Fe(Ⅱ)

JI Mao-Jing ^1, ,
HAI Xin ^2, ,
ZHOU Lu ^2, ,
LIU An-Nan ^2, ,
CUI Zhu-Mei ^{1,
,} ,
BI Sai ^{1,2,
,}

1.
Department of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China;
2.
College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China

Corresponding author: CUI Zhu-Mei, ; BI Sai,

Received Date: 16 February 2022
Revised Date: 12 April 2022
Fund Project:
Supported by the National Natural Science Foundation of China (Nos.22076087, 22004077, 81802923) and the Natural Science Foundation of Shandong Province, China (Nos.ZR2020JQ08, ZR2019MH121).

Abstract: A "turn-on" fluorescence probe was developed based on terephthalic acid functionalized graphene quantum dots (TPA@GQDs) for highly selective detection of Fe²⁺. The TPA@GQDs were prepared by a one-step hydrothermal method using GO as carbon source and terephthalic acid as modifying group, where potassium hydroxide served as the cutting agent and hydrogen peroxide as the auxiliary cutting agent. The structure and component of TPA@GQDs were studied by a variety of characterization methods, and the optical properties and feasibility of TPA@GQDs as a fluorescent probe were further explored. Based on the electron-donating function of Fe²⁺ to induce the fluorescence enhancement of TPA@GQDs, a "turn-on" fluorescent probe was constructed for sensitive detection of Fe²⁺. The linear ranges were from 0.33 to 20 μmol/L and 20 to 60 μmol/L, with the limit of detection (3σ) of 0.33 μmol/L, and the corresponding linear equations were F/F₀=0.0638C+1.1385 (R²=0.9974) and F/F₀=0.0244C+1.9215 (R²=0.9989), respectively. Moreover, this system demonstrated good selectivity toward Fe²⁺, and Fe³⁺ had no effect on the detection of Fe²⁺. Finally, the proposed probe was applied to accurate determination of Fe²⁺ in underground water with recoveries of 98.5%-102.0%, showing broad application prospects in water quality monitoring.

Key words:

1. [1]
  HIDER R C, KONG X. Dalton Trans., 2013, 42(9):3220-3229.HIDER R C, KONG X. Dalton Trans., 2013, 42(9):3220-3229.
2. [2]
  ZHANG Z, XUE W, YANG J, ZHAO Y, GUO J. Anal. Biochem., 2021, 623(1):114171.ZHANG Z, XUE W, YANG J, ZHAO Y, GUO J. Anal. Biochem., 2021, 623(1):114171.
3. [3]
  JIANG M, XU S, YU Y, GAO Y, YIN Z, LI J, ZHANG X, YU H, CHEN B. Spectrochim. Acta, Part A, 2022, 264:120275.JIANG M, XU S, YU Y, GAO Y, YIN Z, LI J, ZHANG X, YU H, CHEN B. Spectrochim. Acta, Part A, 2022, 264:120275.
4. [4]
  LEE Y H, VERWILST P, KIM H S, JU J, KIM J S, KIM K. Chem. Commun., 2019, 55(81):12136-12139.LEE Y H, VERWILST P, KIM H S, JU J, KIM J S, KIM K. Chem. Commun., 2019, 55(81):12136-12139.
5. [5]
  GB5749-2022. Hygienic Standard for Drinking Water. National Standards of the People's Republic of China.生活饮用水卫生标准.中华人民共和国国家标准. GB5749-2022.
6. [6]
  LIU G, LI B, LIU Y, FENG Y, JIA D, ZHOU Y. Appl. Surf. Sci., 2019, 487:1167-1175.LIU G, LI B, LIU Y, FENG Y, JIA D, ZHOU Y. Appl. Surf. Sci., 2019, 487:1167-1175.
7. [7]
  ZHU Y, PAN D, HU X, HAN H, LIN M, WANG C. Sens. Actuators, B, 2017, 243:1-7.ZHU Y, PAN D, HU X, HAN H, LIN M, WANG C. Sens. Actuators, B, 2017, 243:1-7.
8. [8]
  SACMACI S, KARTAL S. Anal. Chim. Acta, 2008, 623(1):46-52.SACMACI S, KARTAL S. Anal. Chim. Acta, 2008, 623(1):46-52.
9. [9]
  CHEN X, JI J, SHI G, XUE Z, ZHOU X, ZHAO L, FENG S. RSC Adv., 2020, 10(54):32897-32905.CHEN X, JI J, SHI G, XUE Z, ZHOU X, ZHAO L, FENG S. RSC Adv., 2020, 10(54):32897-32905.
10. [10]
  SUN Y L, ZHANG X P, ZHAO C X, LIU X, SHU Y, WANG J H, LIU N. Anal. Chim. Acta, 2021, 1183:338973.SUN Y L, ZHANG X P, ZHAO C X, LIU X, SHU Y, WANG J H, LIU N. Anal. Chim. Acta, 2021, 1183:338973.
11. [11]
  XIA C, HAI X, CHEN X W, WANG J H. Talanta, 2017, 168:269-278.XIA C, HAI X, CHEN X W, WANG J H. Talanta, 2017, 168:269-278.
12. [12]
  LIU Y, TU D, ZHU H, MA E, CHEN X. Nanoscale, 2013, 5(4):1369-1384.LIU Y, TU D, ZHU H, MA E, CHEN X. Nanoscale, 2013, 5(4):1369-1384.
13. [13]
  PACINI V A, INGALLINELLA A M, SANGUINETTI G. Water Res., 2005, 39(18):4463-4475.PACINI V A, INGALLINELLA A M, SANGUINETTI G. Water Res., 2005, 39(18):4463-4475.
14. [14]
  DU F, CHENG Z, TAN W, SUN L, RUAN G. Spectrochim. Acta, Part A, 2020, 226:117602.DU F, CHENG Z, TAN W, SUN L, RUAN G. Spectrochim. Acta, Part A, 2020, 226:117602.
15. [15]
  PHAN L M T, HOANG T X, CHO S. Biosensors, 2022, 12(1):41.PHAN L M T, HOANG T X, CHO S. Biosensors, 2022, 12(1):41.
16. [16]
  YAN Y, GONG J, CHEN J, ZENG Z, HUANG W, PU K, LIU J, CHEN P. Adv. Mater., 2019, 31(21):e1808283.YAN Y, GONG J, CHEN J, ZENG Z, HUANG W, PU K, LIU J, CHEN P. Adv. Mater., 2019, 31(21):e1808283.
17. [17]
  HAI X, LI Y, YU K, YUE S, LI Y, SONG W, BI S, ZHANG X. Chin. Chem. Lett., 2021, 32(3):1215-1219.HAI X, LI Y, YU K, YUE S, LI Y, SONG W, BI S, ZHANG X. Chin. Chem. Lett., 2021, 32(3):1215-1219.
18. [18]
  LI M, CHEN T, GOODING J J, LIU J. ACS Sens., 2019, 4(7):1732-1748.LI M, CHEN T, GOODING J J, LIU J. ACS Sens., 2019, 4(7):1732-1748.
19. [19]
  TIAN P, TANG L, TENG K S, LAU S P. Mater. Today Chem., 2018, 10:221-258.TIAN P, TANG L, TENG K S, LAU S P. Mater. Today Chem., 2018, 10:221-258.
20. [20]
  LU H T, LI W J, DONG H F, WEI M L. Small, 2019, 15(36):1902136.LU H T, LI W J, DONG H F, WEI M L. Small, 2019, 15(36):1902136.
21. [21]
  ZHU X, YU J, YAN Y, SONG W, HAI X. Talanta, 2022, 236:122874.ZHU X, YU J, YAN Y, SONG W, HAI X. Talanta, 2022, 236:122874.
22. [22]
  SWEETMAN M J, HICKEY S M, BROOKS D A, HAYBALL J D, PLUSH S E. Adv. Funct. Mater., 2019, 29(14):1808740.SWEETMAN M J, HICKEY S M, BROOKS D A, HAYBALL J D, PLUSH S E. Adv. Funct. Mater., 2019, 29(14):1808740.
23. [23]
  QI B P, HU H, BAO L, ZHANG Z L, TANG B, PENG Y, WANG B S, PANG D W. Nanoscale, 2015, 7(14):5969-5973.QI B P, HU H, BAO L, ZHANG Z L, TANG B, PENG Y, WANG B S, PANG D W. Nanoscale, 2015, 7(14):5969-5973.
24. [24]
  LI L, WU G, YANG G, PENG J, ZHAO J, ZHU J J. Nanoscale, 2013, 5(10):4015-4039.LI L, WU G, YANG G, PENG J, ZHAO J, ZHU J J. Nanoscale, 2013, 5(10):4015-4039.
25. [25]
  HAI X, ZHU X, YU K, YUE S, SONG W, BI S. Biosens. Bioelectron., 2021, 192:113544.HAI X, ZHU X, YU K, YUE S, SONG W, BI S. Biosens. Bioelectron., 2021, 192:113544.
26. [26]
  YAN Ping, LI Zai-Jun. Chin. J. Anal. Chem., 2018, 46(5):670-677.严平,李在均.分析化学, 2018, 46(5):670-677.
27. [27]
  JIANG Yan-Hong, LI Na-Na, LI Zai-Jun. Chin. J. Anal. Chem., 2021, 49(5):809-819.江燕红,李娜娜,李在均.分析化学, 2021, 49(5):809-819.
28. [28]
  HAI X, GUO Z, LIN X, CHEN X, WANG J. ACS Appl. Mater. Interfaces, 2018, 10(6):5853-5861.HAI X, GUO Z, LIN X, CHEN X, WANG J. ACS Appl. Mater. Interfaces, 2018, 10(6):5853-5861.
29. [29]
  HASSAN M, HAQUE E, REDDY K R, MINETT A I, CHEN J, GOMES V G. Nanoscale, 2014, 6(20):11988-11994.HASSAN M, HAQUE E, REDDY K R, MINETT A I, CHEN J, GOMES V G. Nanoscale, 2014, 6(20):11988-11994.
30. [30]
  AMELIA M, FLAMINI R, LATTERINI L. Langmuir, 2010, 26(12):10129-10134.AMELIA M, FLAMINI R, LATTERINI L. Langmuir, 2010, 26(12):10129-10134.
31. [31]
  FUENTE E, MENENDEZ J A, DIEZ M A, SUAREZ D, MONTES-MORAN M A. J. Phys. Chem. B, 2003, 107(26):6350-6359.FUENTE E, MENENDEZ J A, DIEZ M A, SUAREZ D, MONTES-MORAN M A. J. Phys. Chem. B, 2003, 107(26):6350-6359.
32. [32]
  BINOY J, JOE I H, JAYAKUMAR V S. J. Raman Spectrosc., 2005, 36(12):1091-1100.BINOY J, JOE I H, JAYAKUMAR V S. J. Raman Spectrosc., 2005, 36(12):1091-1100.
33. [33]
  LI Y, ZHAO Y, CHENG H, HU Y, SHI G, DAI L, QU L. J. Am. Chem. Soc., 2012, 134(1):15-18.LI Y, ZHAO Y, CHENG H, HU Y, SHI G, DAI L, QU L. J. Am. Chem. Soc., 2012, 134(1):15-18.
34. [34]
  PAN D, ZHANG J, LI Z, WU M. Adv. Mater., 2010, 22(6):734-738.PAN D, ZHANG J, LI Z, WU M. Adv. Mater., 2010, 22(6):734-738.
35. [35]
  HAI X, WANG Y, HAO X, CHEN X, WANG J. Sens. Actuators, B, 2018, 268:61-69.HAI X, WANG Y, HAO X, CHEN X, WANG J. Sens. Actuators, B, 2018, 268:61-69.
36. [36]
  TIAN Y, WANG X, ZHANG D, SHI X, WANG S. J. Photochem. Photobiol., A, 2008, 199(2-3):224-229.TIAN Y, WANG X, ZHANG D, SHI X, WANG S. J. Photochem. Photobiol., A, 2008, 199(2-3):224-229.
37. [37]
  LI C, LI D, MA C, LIU Y. J. Mol. Liq., 2016, 224:83-88.LI C, LI D, MA C, LIU Y. J. Mol. Liq., 2016, 224:83-88.
38. [38]
  ZHUANG Z, BU F, LUO W, PENG H, CHEN S, HU R, QIN A, ZHAO Z, TANG B Z. J. Mater. Chem. C, 2017, 5(7):1836-1842.ZHUANG Z, BU F, LUO W, PENG H, CHEN S, HU R, QIN A, ZHAO Z, TANG B Z. J. Mater. Chem. C, 2017, 5(7):1836-1842.
39. [39]
  WU Y, GUO T, SHU D, ZHANG W, LUAN F, SHI L, GUO D. Luminescence, 2018, 33(5):855-862.WU Y, GUO T, SHU D, ZHANG W, LUAN F, SHI L, GUO D. Luminescence, 2018, 33(5):855-862.
40. [40]
  CHEN T, YANG F, WU X, CHEN Y, YANG G. Carbon, 2020, 167:196-201.CHEN T, YANG F, WU X, CHEN Y, YANG G. Carbon, 2020, 167:196-201.
41. [41]
  WANG Jie-Qing, CHEN Wen-Chuang, ZHANG Jin-Rui. Chem. World, 1997, 38(10):546-548.王洁青,陈文闯,张金锐.化学世界, 1997, 38(10):546-548.

扫一扫看文章

计量

PDF下载量: 9
文章访问数: 941
HTML全文浏览量: 184

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于石墨烯量子点的“Turn-on”型荧光探针用于Fe的选择性检测

通讯作者: 崔竹梅,E-mail:cuizhumei1966@126.com; 毕赛,E-mail:bisai11@126.com

English

raphene Quantum Dots-based Fluorescence “Turn-On” Probe for Selective Detection of Fe(Ⅱ)

Corresponding author: CUI Zhu-Mei, ; BI Sai,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于石墨烯量子点的“Turn-on”型荧光探针用于Fe的选择性检测

通讯作者: 崔竹梅,E-mail:cuizhumei1966@126.com; 毕赛,E-mail:bisai11@126.com

English

raphene Quantum Dots-based Fluorescence “Turn-On” Probe for Selective Detection of Fe(Ⅱ)

Corresponding author: CUI Zhu-Mei, ; BI Sai,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs