气相色谱-飞行时间质谱法非靶向代谢组学解析甲醛降解菌XF-1的代谢轮廓

引用本文: 韩晓红, 韩茜, 王蓉蓉, 史纯珍. 气相色谱-飞行时间质谱法非靶向代谢组学解析甲醛降解菌XF-1的代谢轮廓[J]. 分析化学, 2022, 50(7): 1072-1082. doi: 10.19756/j.issn.0253-3820.221050 shu

Citation: HAN Xiao-Hong, HAN Xi, WANG Rong-Rong, SHI Chun-Zhen. Metabolic Profiling Analysis of Formaldehyde Degrading Strain of XF-1 Using Gas Chromatography-Time of Flight-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2022, 50(7): 1072-1082. doi: 10.19756/j.issn.0253-3820.221050 shu

气相色谱-飞行时间质谱法非靶向代谢组学解析甲醛降解菌XF-1的代谢轮廓

1.
北京工商大学生态环境学院, 北京 100048;
2.
北京工商大学生态环境学院, 国家环境保护食品链污染防治重点实验室, 北京 100048;
3.
北京工商大学生态环境学院, 中国轻工业清洁生产与资源综合利用重点实验室, 北京 100048;
4.
中国科学院生态环境研究中心, 北京 100085

通讯作者: 史纯珍,E-mail:shichunzhen@btbu.edu.cn

基金项目:
国家自然科学基金项目(No.52170106)和北京市教委青年拔尖人才项目(No.CIT&TCD201904032)资助。

摘要: 利用从油烟冷凝液中筛出的一株解淀粉芽孢杆菌XF-1,采用生物法高效降解烹饪油烟中的甲醛。通过前期的研究发现,当甲醛为唯一碳源时,该菌株受到生长抑制而出现降解甲醛效率降低的现象。本研究基于气相色谱-飞行时间质谱(GC-TOF-MS)的非靶向代谢组学方法,比较了不同碳源在不同培养时间下XF-1的代谢轮廓差异。结果表明,不同碳源培养会导致菌株XF-1代谢发生显著变化,当甲醛为唯一碳源时,与葡萄糖组相比出现了生长抑制现象,最终导致代谢过程延后,其中,胱氨酸、天冬氨酸和谷氨酰胺的浓度变化抑制了细胞正常增殖与生长。本研究通过基于GC-TOF-MS的非靶向代谢组学研究,解析了XF-1菌株受到生长抑制的原因,为提高微生物对烹饪油烟的降解效率提供了参考。

关键词:

English

Metabolic Profiling Analysis of Formaldehyde Degrading Strain of XF-1 Using Gas Chromatography-Time of Flight-Mass Spectrometry

1.
School of Ecology and Environment, Beijing Technology and Business University, Beijing 100048, China;
2.
State Environmental Protection Key Laboratory of Food Chain Pollution Control, Beijing Technology and Business University, Beijing 100048, China;
3.
Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing 100048, China;
4.
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China

Corresponding author: SHI Chun-Zhen, shichunzhen@btbu.edu.cn

Received Date: 25 January 2022
Revised Date: 21 April 2022
Fund Project:
Supported by the National Natural Science Foundation of China (No.52170106) and the High-level Teacher in Beijing Municipal Universities in the Period of 13th Five-year Plan (No.CIT&TCD201904032).

Abstract: Cooking oil fumes are widely concerned because of the complex composition and the hard-to-degrade characterization. One of the most important pollutants in cooking oil fumes is formaldehyde which is a hazard to human health. In this study, a strain of Bacillus amyloliticus XF-1 screened out in previous work was used for efficiently degrading formaldehyde from the condensate of cooking oil fumes. The previous study found that when formaldehyde was the only carbon source, the biodegradation rate was reduced owing to the inhibition effect to the strain. It was necessary to investigate the metabolic mechanism of strain XF-1 and to illustrate the reason for growth inhibition. The metabolic profiling of XF-1 with different carbon sources and under different culture time conditions was analyzed by gas chromatography-time of flight-mass spectrometry (GC-TOF-MS). The results showed that different carbon sources caused different metabolic profiles of XF-1. The metabolic process was delayed using formaldehyde as the carbon source. The concentrations of cystine, aspartic acid and glutamine were significantly changed, which showed that the cell growth and proliferation were inhibited. This work provided a reference for improving the biodegradation efficiency of cooking oil fumes in the future.

Key words:

Bacillus amyloliquefaciens
/ Formaldehyde degrading strain
/ Metabolomics
/ Gas chromatography-time of flight-mass spectrometry

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

气相色谱-飞行时间质谱法非靶向代谢组学解析甲醛降解菌XF-1的代谢轮廓

通讯作者: 史纯珍,E-mail:shichunzhen@btbu.edu.cn

English

Metabolic Profiling Analysis of Formaldehyde Degrading Strain of XF-1 Using Gas Chromatography-Time of Flight-Mass Spectrometry

Corresponding author: SHI Chun-Zhen, shichunzhen@btbu.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

气相色谱-飞行时间质谱法非靶向代谢组学解析甲醛降解菌XF-1的代谢轮廓

通讯作者: 史纯珍,E-mail:shichunzhen@btbu.edu.cn

English

Metabolic Profiling Analysis of Formaldehyde Degrading Strain of XF-1 Using Gas Chromatography-Time of Flight-Mass Spectrometry

Corresponding author: SHI Chun-Zhen, shichunzhen@btbu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs