基于扫描电化学显微镜的微生物电化学研究进展

引用本文: 田晓春, 李祎頔, 潘琴, 赵峰. 基于扫描电化学显微镜的微生物电化学研究进展[J]. 分析化学, 2021, 49(6): 858-866,906. doi: 10.19756/j.issn.0253-3820.211187 shu

Citation: TIAN Xiao-Chun, LI Yi-Di, PAN Qin, ZHAO Feng. Research Progress and Prospects of Microbial Electrochemistry Based on Scanning Electrochemical Microscopy[J]. Chinese Journal of Analytical Chemistry, 2021, 49(6): 858-866,906. doi: 10.19756/j.issn.0253-3820.211187 shu

基于扫描电化学显微镜的微生物电化学研究进展

中国科学院城市环境研究所, 中国科学院城市污染物转化重点实验室, 厦门 361021

通讯作者: 赵峰,E-mail:fzhao@iue.ac.cn

基金项目:
国家自然科学基金项目（No.21802133）、中国博士后科学基金项目（No.2018M642574）、中国科学院城市污染物转化联合项目（No.KLUPC-2020-5）和海峡博士后交流资助计划资助。

摘要: 微生物是自然界中广泛存在的生命体，它们通过呼吸代谢转化碳水化合物并产生能量的过程与电子转移密切相关，在元素的生物地球化学循环与物质能量转化过程中发挥着关键作用。因此，微生物电化学的原理及其应用近年来备受关注。电化学及其联用技术能够从电子转移层面揭示微生物/非生物界面物质和能量转化机制，是直接、有效的分析手段。其中，扫描电化学显微镜（Scanning electrochemical microscopy，SECM）是一种基于氧化还原反应的空间成像技术，可以原位监测微区环境中的电化学反应信号，具有灵敏高、空间分辨率高等优点。SECM在微观层面揭示微生物电子传递机制方面也发挥了重要作用，涵括了原位监测微生物呼吸活性、产电能力以及生物膜的微区环境（如pH、溶解氧、H₂O₂浓度）等。本文基于SECM工作原理，详细阐述了其在微生物电子传递机制方面的研究进展，归纳总结了微生物电化学在微生物芯片、生物电化学系统、金属腐蚀等领域的应用潜力，并基于SECM的拓展技术，展望了微生物电化学的发展趋势以及所面临的机遇和挑战。

关键词:

English

Research Progress and Prospects of Microbial Electrochemistry Based on Scanning Electrochemical Microscopy

CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China

Corresponding author: ZHAO Feng, fzhao@iue.ac.cn

Received Date: 15 March 2021
Revised Date: 28 April 2021
Fund Project:
Supported by the National Natural Science Foundation of China (No.21802133), the China Postdoctoral Science Foundation (No.2018M642574), the CAS Key Laboratory of Urban Pollutant Conversion Joint Research Fund (No.KLUPC-2020-5) and the Straits Post-doctoral Exchange Programme of Fujian Province.

Abstract: Microbes widely exist in nature and possess the ability to transform carbon compounds into energy through respiratory metabolism, which are important for element biogeochemical cycle, matters and energy conversion via redox reactions and electron transfer. Electrochemical and its integrated technologies are convenient in revealing the electron transfer mechanism at biotic-abiotic interface. Scanning electrochemical microscopy (SECM) is a scanning probe technology for visualizing the local redox activity of a surface with a high sensitivity and spatial resolution, and it enables in situ monitor microbial respiratory activity, electricity generation ability and micro-environmental factors of biofilms. On the basis of the composition and working principles, this review discussed the functional role of SECM for in situ revealing the electron transfer mechanism, summarized the researches in applications fields of microbial chips, bio-electrochemical systems and metal corrosion, and finally made a conclusion on the opportunities and challenges of the microbial electrochemistry in the future.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于扫描电化学显微镜的微生物电化学研究进展

通讯作者: 赵峰,E-mail:fzhao@iue.ac.cn

English

Research Progress and Prospects of Microbial Electrochemistry Based on Scanning Electrochemical Microscopy

Corresponding author: ZHAO Feng, fzhao@iue.ac.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于扫描电化学显微镜的微生物电化学研究进展

通讯作者: 赵峰,E-mail:fzhao@iue.ac.cn

English

Research Progress and Prospects of Microbial Electrochemistry Based on Scanning Electrochemical Microscopy

Corresponding author: ZHAO Feng, fzhao@iue.ac.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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