细胞顶空气中典型醛酮化合物定量分析方法研究

引用本文: 尹志红, 陈钊铭, 陈星, 杨子峰, 李征途, 叶枫, 张志娟, 方明亮, 李雪. 细胞顶空气中典型醛酮化合物定量分析方法研究[J]. 分析化学, 2021, 49(8): 1375-1383. doi: 10.19756/j.issn.0253-3820.211072 shu

Citation: YIN Zhi-Hong, CHEN Zhao-Ming, CHEN Xing, YANG Zi-Feng, LI Zheng-Tu, YE Feng, ZHANG Zhi-Juan, FANG Ming-Liang, LI Xue. Quantitative Analysis of Typical Aldehydes and Ketones in Headspace Air of Cell[J]. Chinese Journal of Analytical Chemistry, 2021, 49(8): 1375-1383. doi: 10.19756/j.issn.0253-3820.211072 shu

细胞顶空气中典型醛酮化合物定量分析方法研究

1.
暨南大学质谱仪器与大气环境研究所, 广州 510632;
2.
广东省大气污染在线源解析系统工程技术研究中心, 广州 510632;
3.
广州医科大学呼吸疾病国家重点实验室, 广州 511436;
4.
广州医科大学附属第一医院, 国家呼吸系统疾病临床医学研究中心, 广州 510120;
5.
河南中医药大学药学院, 郑州 450008;
6.
南洋理工大学土木与环境工程学院, 新加坡 637457

通讯作者: 李雪,E-mail:tamylee@jnu.edu.cn

基金项目:
广东特支计划本土创新创业团队项目（No.2019BT02Z546）、呼吸疾病国家重点实验室开放课题项目（No.SKLRD-OP-201913）和中国科学院地球科学领域核心关键技术攻关预研项目（No.282021000003）资助。

摘要: 采用2，4-二硝基苯肼衍生柱收集挥发性醛酮类组分，转化为稳定的醛酮2，4-二硝基苯腙有机物，采用超高效液相色谱-高分辨质谱联用技术进行分析，建立了22种典型醛酮类化合物定量分析方法。2，4-二硝基苯腙有机物在高（40~1000 μg/L）、中（8~400 μg/L）、低（0.08~8 μg/L）浓度范围内标准曲线线性良好，相关系数R²>0.998，检出限范围为0.003~0.09 μg/L；高、中、低浓度范围的标准溶液的日内及日间精密度（RSD）均小于10%；回收率在95.1%~109.6%之间，RSD≤5.9%。将本方法用于分析A549细胞以及甲型H1N1流感病毒（IAV）感染的A549细胞顶空气中醛酮类物质，检测到19种醛酮类物质，通过正交偏最小二乘-判别分析法分析检测结果，发现两组醛酮代谢物存在显著差别，并筛选出11种潜在的醛酮生物标记物

关键词:

English

Quantitative Analysis of Typical Aldehydes and Ketones in Headspace Air of Cell

1.
Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou 510632, China;
2.
Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, Guangzhou 510632, China;
3.
State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 511436, China;
4.
National Clinical Research Center for Respiratory Disease, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China;
5.
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450008, China;
6.
School of Civil and Environmental Engineering, Nanyang Technological University, Singapore 637457, Singapore

Corresponding author: LI Xue, tamylee@jnu.edu.cn

Received Date: 26 January 2021
Revised Date: 01 April 2021
Fund Project:
Supported by the "Guangdong Special Support Plan" Local Innovation and Entrepreneurship Team Project (No.2019BT02Z546), the Open Project of the State Key Laboratory of Respiratory Diseases (No.SKLRD-OP-201913), the Pre-research Project of Core Key Technologies in the Field of Earth Sciences, Chinese Academy of Sciences (No.282021000003).

Abstract: Aldehydes and ketones in breath can reflect the oxidative stress response of the cells in the body. A better understanding of the aldehydes and ketones emitted from the cells is the key to revealing the mechanism of breath test for the diagnosis of oxidative stress-related diseases. In this study, a quantitative method was developed for 22 typical aldehyde and ketone metabolites. Standard compounds were transformed into stable DNPH-carbonyl adducts by using 2,4-dinitrophenylhydrazine (DNPH) impregnated cartridge. These adducts were further eluted and analyzed by ultra high performance liquid chromatography coupled with high resolution mass spectrometry. The standard curves of 22 DNPH-carbonyl adducts showed linearity at high (40-1000 μg/L), medium (8-400 μg/L) and low (0.08-8 μg/L) levels, respectively. The coefficient of correlation (R²) was >0.998 and the detection limits were 0.003-0.09 μg/L. The method demonstrated good precision, e.g. intra-day RSD <3.92% and inter-day RSD <5.3%, as well as satisfactory recoveries of 95.1%-109.6% (RSD<5.9%). The method was further applied to the analysis of aldehydes and ketones in the headspace of A549 cells and influenza A (H1N1) infected A549 cells. The experimental data were analyzed by using orthogonal partial least squares discriminant analysis, and a total of 19 aldehydes and ketones were quantified and 11 potential biomarkers were screened for the diagnosis of IAV infection.

Key words:

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

细胞顶空气中典型醛酮化合物定量分析方法研究

通讯作者: 李雪,E-mail:tamylee@jnu.edu.cn

English

Quantitative Analysis of Typical Aldehydes and Ketones in Headspace Air of Cell

Corresponding author: LI Xue, tamylee@jnu.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

细胞顶空气中典型醛酮化合物定量分析方法研究

通讯作者: 李雪,E-mail:tamylee@jnu.edu.cn

English

Quantitative Analysis of Typical Aldehydes and Ketones in Headspace Air of Cell

Corresponding author: LI Xue, tamylee@jnu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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