人体呼出挥发性有机物的口罩微萃取气相色谱-质谱分析

引用本文: 陈伟妮, 袁子程, 蔡深回, 邹颖通, 辛国斌, 狄丹丹, 宋昕, 赵鹏, 吴曼曼, 胡斌. 人体呼出挥发性有机物的口罩微萃取气相色谱-质谱分析[J]. 分析化学, 2022, 50(3): 445-453. doi: 10.19756/j.issn.0253-3820.210828 shu

Citation: CHEN Wei-Ni, YUAN Zi-Cheng, CAI Shen-Hui, ZOU Ying-Tong, XIN Guo-Bin, DI Dan-Dan, SONG Xin, ZHAO Peng, WU Man-Man, HU Bin. Gas Chromatography-Mass Spectrometry Analysis of Human Exhaled Volatile Organic Compounds via Wearable Facemask Microextraction Sampling[J]. Chinese Journal of Analytical Chemistry, 2022, 50(3): 445-453. doi: 10.19756/j.issn.0253-3820.210828 shu

人体呼出挥发性有机物的口罩微萃取气相色谱-质谱分析

陈伟妮 ^1, ,
袁子程 ^1, ,
蔡深回 ^1, ,
邹颖通 ^2, ,
辛国斌 ^3, ,
狄丹丹 ^1, ,
宋昕 ^4, ,
赵鹏 ^5, ,
吴曼曼 ^{2,
,} ,
胡斌 ^{1,
,}

¹暨南大学质谱仪器与大气环境研究所, 广东省大气污染在线源解析系统工程技术研究中心, 广州 510632;
²广州禾信仪器股份有限公司, 广州 510530;
³法庭毒物分析公安部重点实验室(北京市公安局), 北京 100192;
⁴合肥市公安局, 合肥 230001;
⁵宁波大学高等技术研究院, 宁波 315210

通讯作者: 吴曼曼,E-mail:mm.wu@hxmass.com; 胡斌,E-mail:bin.hu@jnu.edu.cn

基金项目:
国家自然科学基金项目（No.21804053）和法庭毒物分析公安部重点实验室（北京市公安局）开放基金项目（No.2021FTDWFX03）资助。

摘要: 人体呼气中含有大量与人类行为和健康状况有关的挥发性有机物（VOCs）。本研究采用口罩微萃取方法富集人体呼出VOCs，并联用3种气相色谱-质谱仪（GC-MS）对呼出VOCs进行分析鉴定与比对。优化了固相微萃取探针及富集采样时间；通过对比正常生理呼出VOCs和口罩的背景信号，探索了人体经过不同的食物摄入和口腔护理对呼出VOCs的影响，以及特征VOCs的可检测性和持续性；对比了常规、便携式和全二维GC-MS仪器对人体呼出VOCs的分析性能与特性。结果表明，口罩微萃取气相色谱-质谱方法可有效地检测口腔中大量的内源性和外源性挥发VOCs，并且具有采样安全简便、高效便捷、与不同仪器的适配性强等特点，为人体口腔健康与疾病诊断提供了新方法。

关键词:

English

Gas Chromatography-Mass Spectrometry Analysis of Human Exhaled Volatile Organic Compounds via Wearable Facemask Microextraction Sampling

¹Guangdong Provincial Engineering Research Center for Online Source Apportionment System of Air Pollution, Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou 510632, China;
²Guangzhou Hexin Instrument Co., Ltd, Guangzhou 510530, China;
³Key Laboratory of Forensic Toxicology, Ministry of Public Security, Beijing Municipal Public Security Bureau, Beijing 100192, China;
⁴Hefei Public Security Bureau, Hefei 230001, China;
⁵The Research Institute of Advanced Technologies, Ningbo University, Ningbo 315210, China

Corresponding author: WU Man-Man, ; HU Bin,

Received Date: 05 November 2021
Revised Date: 04 January 2022
Fund Project:
Supported by the National Natural Science Foundation of China (No.21804053) and the Key Laboratory of Forensic Toxicology, Ministry of Public Security, People's Republic of China (Beijing Municipal Public Security Bureau) (No.2021FTDWFX03).

Abstract: Human exhaled breath contains a large number of volatile metabolites relating to human behavior and health states. In this study, solid phase microextraction (SPME) fibers were directly inserted into a facemask (SPME-in-facemask) to form a new facemask microextraction device for extracting human exhaled volatile organic compounds (VOCs). After sampling, SPME fibers were then coupled with different gas chromatography-mass spectrometers (GC-MS) for further identification of extracted VOCs, including conventional GC-MS, portable GC-MS and comprehensive two-dimensional GC-MS. Different SPME fibers and sampling time were investigated and compared. By comparing the background signals of face mask human exhaled VOCs, the effects of food intake and oral care on the compositions and changes of human exhaled VOCs were explored. The detectability and persistence of exhaled exogenous VOCs were investigated. The results showed that the facemask microextraction sampling coupled with GC-MS could be an efficient analytical tool for detecting a large amount of exhaled endogenous and exogenous VOCs. With the unique features of the facemask microextraction device, including safety, simplicity, convenient sampling, and strong adaptability to couple with different GC-MS methods in which portable GC-MS could provide a fast screening within minutes, while GC×GC-MS could give a comprehensive analysis for biomarker discovery. This work presented a novel promising strategy for investigating human health and oral diseases.

Key words:

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人体呼出挥发性有机物的口罩微萃取气相色谱-质谱分析

通讯作者: 吴曼曼,E-mail:mm.wu@hxmass.com; 胡斌,E-mail:bin.hu@jnu.edu.cn

English

Gas Chromatography-Mass Spectrometry Analysis of Human Exhaled Volatile Organic Compounds via Wearable Facemask Microextraction Sampling

Corresponding author: WU Man-Man, ; HU Bin,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

人体呼出挥发性有机物的口罩微萃取气相色谱-质谱分析

通讯作者: 吴曼曼,E-mail:mm.wu@hxmass.com; 胡斌,E-mail:bin.hu@jnu.edu.cn

English

Gas Chromatography-Mass Spectrometry Analysis of Human Exhaled Volatile Organic Compounds via Wearable Facemask Microextraction Sampling

Corresponding author: WU Man-Man, ; HU Bin,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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