解聚集诱导发射探针用于人端粒i-Motif二聚体与单体的选择性识别

引用本文: 曾瑞迪, 杨通, 陈佳汇, 王丹丹, 周小顺, 邵勇. 解聚集诱导发射探针用于人端粒i-Motif二聚体与单体的选择性识别[J]. 分析化学, 2021, 49(6): 992-998. doi: 10.19756/j.issn.0253-3820.210444 shu

Citation: ZENG Rui-Di, YANG Tong, CHEN Jia-Hui, WANG Dan-Dan, ZHOU Xiao-Shun, SHAO Yong. Selective Recognition of Human Telomeric i-Motif Dimer Over Monomer Using A Disaggregation-Induced Emissive Probe[J]. Chinese Journal of Analytical Chemistry, 2021, 49(6): 992-998. doi: 10.19756/j.issn.0253-3820.210444 shu

解聚集诱导发射探针用于人端粒i-Motif二聚体与单体的选择性识别

浙江师范大学化学与生命科学学院, 金华 321004

通讯作者: 邵勇,E-mail:yshao@zjnu.cn

基金项目:
浙江省自然科学基金重点项目（No.LZ20B050001）、浙江省万人计划项目（No.2020R52022）、国家自然科学基金项目（No.21675142）、国家级大学生创新创业计划项目（No.201910345027）和浙江师范大学自主设计科研项目（No.2020ZS0304）资助。

摘要: 由于具有重要的生物活性，并在生物传感器和材料化学等领域广泛应用，i-motif结构开关及识别引起越来越多的关注。分子内i-motif多聚体具有多个重复结构单元，其与i-motif单体的周围环境存在差异。虽然有关i-motif单体的探针识别已有报道，但i-motif多聚体相对于单体的探针选择性识别仍是亟待解决的问题。本研究发现，金丝桃素（Hyp）能选择性区分人端粒分子内i-motif二聚体与单体。二聚体的结合使Hyp的非荧光聚集体发生解聚，产生Turn-on荧光响应，然而Hyp的结合对二聚体结构的稳定性及构象无影响。链接体（Linker）长度实验表明，二聚体中两个i-motif结构单元间的空间可能为Hyp的结合位点，Hyp通过完全氢键互补作用与Loop中的胸腺嘧啶发生结合作用。此结合空间只有在酸性条件下形成i-motif二聚体结构时才能有效结合Hyp。本研究构建的Hyp荧光探针在i-motif多聚体的识别研究中具有重要的作用。

关键词:

English

Selective Recognition of Human Telomeric i-Motif Dimer Over Monomer Using A Disaggregation-Induced Emissive Probe

College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China

Corresponding author: SHAO Yong, yshao@zjnu.cn

Received Date: 16 April 2021
Revised Date: 10 May 2021
Fund Project:
Supported by the Key Project of Natural Science Foundation of Zhejiang Province (No.LZ20B050001), the Leading Talent Program of Science and Technology Innovation in Zhejiang (No.2020R52022), the National Natural Science Foundation of China (No.21675142), the National Undergraduate Training Program for Innovation and Entrepreneurship (No.201910345027), and the Independent Designing Scientific Research Project of Zhejiang Normal University (No.2020ZS0304).

Abstract: Due to the important biological activity and wide applications in biosensors and materials chemistry, i-motif structure switching and recognition have attracted more and more attention. In addition, intramolecular i-motif multimers have multiple repeating structural units, which are different from the surrounding environment of i-motif monomers. Although recognition of i-motif monomers by probes has been reported, the selective recognition of i-motif multimers over monomers has yet to be explored. In this work, it is found that hypericin (Hyp) can selectively distinguish human telomeric i-motif dimer from its monomer. The binding of the i-motif dimer depolymerizes the Hyp aggregation and causes a turn-on fluorescence response. However, the Hyp binding has no effect on the stability and conformation of the dimer structure. Since Hyp has a hydrogen bond pattern that is completely complementary to two thymines, the linker length experiment indicates that the in-between space of the two i-motif structural units in the dimer may be the binding site of Hyp via the hydrogen bonding with thymines from the loops. This binding space can effectively bind Hyp only when the i-motif dimer structure is formed under acidic conditions. This work demonstrates that Hyp as a fluorescent probe will play an important role in recognizing i-motif multimers.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

解聚集诱导发射探针用于人端粒i-Motif二聚体与单体的选择性识别

通讯作者: 邵勇,E-mail:yshao@zjnu.cn

English

Selective Recognition of Human Telomeric i-Motif Dimer Over Monomer Using A Disaggregation-Induced Emissive Probe

Corresponding author: SHAO Yong, yshao@zjnu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

解聚集诱导发射探针用于人端粒i-Motif二聚体与单体的选择性识别

通讯作者: 邵勇,E-mail:yshao@zjnu.cn

English

Selective Recognition of Human Telomeric i-Motif Dimer Over Monomer Using A Disaggregation-Induced Emissive Probe

Corresponding author: SHAO Yong, yshao@zjnu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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