血清中可溶性白介素-2受体胶体金免疫层析试纸检测方法研究

引用本文: 徐凤琴, 朱群艳, 张婳, 王振新. 血清中可溶性白介素-2受体胶体金免疫层析试纸检测方法研究[J]. 分析化学, 2021, 49(6): 973-981. doi: 10.19756/j.issn.0253-3820.201723 shu

Citation: XU Feng-Qin, ZHU Qun-Yan, ZHANG Hua, WANG Zhen-Xin. Preparation of Colloidal Gold-Labeled Immunochromatographic Strips for Detection of Soluble Interleukin-2 Receptors in Real Samples[J]. Chinese Journal of Analytical Chemistry, 2021, 49(6): 973-981. doi: 10.19756/j.issn.0253-3820.201723 shu

血清中可溶性白介素-2受体胶体金免疫层析试纸检测方法研究

徐凤琴 ^1,2, ,
朱群艳 ^1, ,
张婳 ^1, ,
王振新 ^{1,2,
,}

1.
中国科学院长春应用化学研究所电分析化学国家重点实验室, 长春 130022;
2.
中国科学技术大学应用化学与工程学院, 合肥 230026

通讯作者: 王振新,E-mail:wangzx@ciac.ac.cn

基金项目:
国家自然科学基金项目（No.61901438）资助。

摘要: 快速、灵敏地检测血清中的可溶性白介素-2受体（sIL-2R）对相关疾病的临床诊断至关重要。本研究建立了一种基于胶体金免疫层析试纸条的快速、定量检测实际样品中sIL-2R的方法。在硝酸纤维膜上分别包被羊抗兔二抗（2ndAb）和兔抗鼠sIL-2R单克隆抗体（cAb）作为质控线（C线）和检测线（T线）；以兔抗鼠sIL-2R单克隆抗体（dAb）修饰的金纳米粒子为标记物，采用双抗体夹心法制备用于sIL-2R检测的免疫层析试纸条。合成了3种不同形貌和粒径的金纳米粒子，考察了其对胶体金标记免疫层析试纸条检测性能的影响。实验结果表明，胶体金标记免疫层析试纸条对sIL-2R的检测性能与金纳米粒子的形貌和粒径密切相关；其中，金纳米棒（Gold nanorod，GNR）标记的免疫层析试纸条具有较宽的线性范围（1~6250 ng/mL）和较低的检出限（1.0 ng/mL）。采用GNR免疫层析试纸条成功实现了荷三阴性乳腺肿瘤（MDA-MB-231） BALB/c裸鼠血清中sIL-2R浓度的检测，结果表明，sIL-2R浓度与肿瘤进程呈正相关。

关键词:

English

Preparation of Colloidal Gold-Labeled Immunochromatographic Strips for Detection of Soluble Interleukin-2 Receptors in Real Samples

1.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2.
School of Applied Chemical Engineering, University of Science and Technology of China, Hefei 230026, China

Corresponding author: WANG Zhen-Xin, wangzx@ciac.ac.cn

Received Date: 03 December 2020
Revised Date: 18 March 2021
Fund Project:
Supported by the National Natural Science Foundation of China (No.61901438).

Abstract: Rapid and sensitive detection of soluble interleukin-2 receptor (sIL-2R) in serum is believed to be of vital importance in clinical diagnosis. In this study, a colloidal gold-labeled immunochromatographic strip was developed for rapid and quantitative detection of sIL-2R in real sample. The goat anti-rabbit secondary antibody (2ndAb) and rabbit anti-mouse sIL-2R monoclonal antibody (cAb) were sprayed onto the nitrocellulose membrane as control line (C line) and test line (T line), respectively. The rabbit anti-mouse sIL-2R monoclonal antibody (dAb) modified gold nanoparticles were used as markers to fabricate the immunochromatographic strip for detection of sIL-2R by a sandwich method. Three gold nanoparticles with different sizes and morphologies were synthesized, and their effects on the detection performance of colloidal gold-labeled immunochromatographic strip were investigated. The experimental results showed that the detection performance of colloidal gold-labeled immunochromatographic strip for sIL-2R was closely related to the morphology and particle size of gold nanoparticles. Among them, gold nanorod (GNR)-based immunochromatographic strip had the widest linear range (1-6250 ng/mL) and the lowest detection limit (1.0 ng/mL). In addition, the concentrations of sIL-2R in the sera of triple-negative breast tumors (MDA-MB-231)-bearing BALB/c nude mice were successfully detected using GNR-based immunochromatographic strips, and it was found that the concentration of sIL-2R was positively correlated with tumor progression.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

血清中可溶性白介素-2受体胶体金免疫层析试纸检测方法研究

通讯作者: 王振新,E-mail:wangzx@ciac.ac.cn

English

Preparation of Colloidal Gold-Labeled Immunochromatographic Strips for Detection of Soluble Interleukin-2 Receptors in Real Samples

Corresponding author: WANG Zhen-Xin, wangzx@ciac.ac.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

血清中可溶性白介素-2受体胶体金免疫层析试纸检测方法研究

通讯作者: 王振新,E-mail:wangzx@ciac.ac.cn

English

Preparation of Colloidal Gold-Labeled Immunochromatographic Strips for Detection of Soluble Interleukin-2 Receptors in Real Samples

Corresponding author: WANG Zhen-Xin, wangzx@ciac.ac.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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