云母表面纳米水层中Aβ16-22自组装的研究

引用本文: 陈文勇, 王建华, 杨甜甜, 霍庚阳, 徐薇, 李宾, 周星飞. 云母表面纳米水层中Aβ16-22自组装的研究[J]. 分析化学, 2021, 49(4): 611-617. doi: 10.19756/j.issn.0253-3820.201696 shu

Citation: CHEN Wen-Yong, WANG Jian-Hua, YANG Tian-Tian, HUO Geng-Yang, XU Wei, LI Bin, ZHOU Xing-Fei. Self-assembly of Aβ16-22 in Water Nanofilm on Mica Surface[J]. Chinese Journal of Analytical Chemistry, 2021, 49(4): 611-617. doi: 10.19756/j.issn.0253-3820.201696 shu

云母表面纳米水层中Aβ16-22自组装的研究

陈文勇 ^1, ,
王建华 ^1,2, ,
杨甜甜 ^1, ,
霍庚阳 ^1, ,
徐薇 ^1, ,
李宾 ^2, ,
周星飞 ^{1,
,}

1.
宁波大学物理科学与技术学院, 宁波 315211;
2.
中国科学院上海高等研究院基础交叉研究中心, 上海 201210

通讯作者: 周星飞,E-mail:zhouxingfei@nbu.edu.cn

基金项目:
国家自然科学基金项目（Nos.31670871，12074208）、浙江省自然科学基金项目（No.LY18A040003）和宁波市自然科学基金项目（No.2018A610220）资助

摘要: 淀粉样多肽在"拥挤"环境中的自组装与神经退行性疾病密切相关。本研究在恒温高湿度密闭环境中，获得了云母表面厚度可控的纳米水层，并详细研究了Aβ16-22在纳米水层中的扩散和聚集行为。结果表明，淀粉样多肽可以在纳米水层中扩散，并形成不同高度的纳米片层，而且样品的培养温度和多肽浓度对纳米片层的结构和形态具有决定性的影响。原子力显微镜峰值力定量模式（PeakForce-QNM）测试表明，纳米水层中形成的Aβ16-22纳米片层的弹性模量约为（5.0±0.4）GPa，明显大于溶液中形成的淀粉样纤维的弹性模量（（3.1±0.2）GPa）。纳米红外光谱成像显微镜（NanoIR）分析结果表明，云母表面水层中形成的纳米片层的二级结构主要是α-helix，不同于纤维中的β-sheet结构，这可能是引起两者压缩弹性模量差异的原因。

关键词:

English

Self-assembly of Aβ16-22 in Water Nanofilm on Mica Surface

1.
School of Physics Science and Technology, Ningbo University, Ningbo 315211, China;
2.
Basic Cross-research Center, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China

Corresponding author: ZHOU Xing-Fei, zhouxingfei@nbu.edu.cn

Received Date: 23 November 2020
Revised Date: 20 January 2021
Fund Project:
Supported by the National Natural Science Foundation of China (Nos.31670871, 12074208), the Natural Science Foundation of Zhejiang Province, China (No.LY18A040003) and the Natural Science Foundation of Ningbo, China (No.2018A610220).

Abstract: The self-assembly of amyloid peptides in crowded environment has attracted tremendous attention due to its close relation to human neurological diseases. The experiments investigated the aggregation and diffusion behavior of Aβ16-22 in the water nanofilm adsorbed on the surface of mica substrate under a closed environment. The result showed that the peptide molecules could diffuse and aggregate in water layer and formed peptide nanosheets with different heights under various conditions, and the nanosheet formation was remarkably affected by the incubation temperature and peptide concentration. The elastic modulus of Aβ16-22 nanosheets was further evaluated by PeakForce-QNM, and it was found that the elastic modulus were estimated to be about (5.0±0.4) GPa, notably stiffer than that of amyloid fibrils ((3.1±0.2) GPa) formed in bulk solution. NanoIR analysis indicated that the secondary structure of nanosheets adsorbed on mica surface was mainly related to α-helix conformations, unlike the β-sheet structure of amyloid fibrils. We attributed the discrepancy of compression elasticity to the difference of peptide second structure of these two species.

Key words:

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

云母表面纳米水层中Aβ16-22自组装的研究

通讯作者: 周星飞,E-mail:zhouxingfei@nbu.edu.cn

English

Self-assembly of Aβ16-22 in Water Nanofilm on Mica Surface

Corresponding author: ZHOU Xing-Fei, zhouxingfei@nbu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

云母表面纳米水层中Aβ16-22自组装的研究

通讯作者: 周星飞,E-mail:zhouxingfei@nbu.edu.cn

English

Self-assembly of Aβ16-22 in Water Nanofilm on Mica Surface

Corresponding author: ZHOU Xing-Fei, zhouxingfei@nbu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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