分子印迹传感器能量转移电化学发光法检测赤霉素

引用本文: 谢汉钊, 杨斌, 李建平. 分子印迹传感器能量转移电化学发光法检测赤霉素[J]. 分析化学, 2020, 48(12): 1633-1641. doi: 10.19756/j.issn.0253-3820.201179 shu

Citation: XIE Han-Zhao, YANG Bin, LI Jian-Ping. A Molecularly Imprinted Electrochemical Luminescence Sensor for Detection of Gibberellin Based on Energy Transfer[J]. Chinese Journal of Analytical Chemistry, 2020, 48(12): 1633-1641. doi: 10.19756/j.issn.0253-3820.201179 shu

分子印迹传感器能量转移电化学发光法检测赤霉素

桂林理工大学化学与生物工程学院, 广西电磁化学功能物质重点实验室, 桂林 541004

通讯作者: 李建平, likianping@263.net

基金项目:
本文系国家自然科学基金项目（No.21765006）、广西自然科学基金项目（No.2018GXNSFAA138145）和广西岩溶地区水污染控制与用水安全保障协同创新中心项目资助

摘要: 制备了赤霉素（GA3）分子印迹传感器，建立了基于共振能量转移原理增强Ru（bpy）₃Cl₂电化学发光（ECL）信号超灵敏测定GA3的方法。在玻碳电极表面滴涂掺杂Au的g-C₃N₄材料（g-C₃N₄/Au），进一步电化学聚合邻苯二胺，得到分子印迹聚合物（MIP）膜，采用甲醇-乙酸洗脱液洗脱后，得到可特异性识别GA3的空穴。以Ru（bpy）₃²⁺为探针，g-C₃N₄/Au为能量供体，利用二者间共振能量转移增强Ru（bpy）₃²⁺的ECL强度。同时，ECL共振能量转移与分子印迹技术结合，提高传感器的选择性。随着样品中GA3浓度增加，分子印迹空穴与GA3重新结合，导致ECL强度逐渐降低。此传感器检测GA3的线性范围为4.0×10^-14~7.0×10^-11 mol/L，检出限为1.64×10^-14 mol/L。将此传感器用于啤酒中GA3检测，回收率为95.7%~103.7%。

关键词:

English

A Molecularly Imprinted Electrochemical Luminescence Sensor for Detection of Gibberellin Based on Energy Transfer

College of Chemical and Bioengineering, Guilin University of Technology, Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, Guilin 541004, China

Corresponding author: LI Jian-Ping, likianping@263.net

Received Date: 05 April 2020
Revised Date: 08 August 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 21765006), the Guangxi Natural Science Foundation of China (No. 2018GXNSFAA138145) and Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, China.

Abstract: A molecularly imprinted sensor was fabricated, and a method based on enhancing electrochemiluminescence (ECL) signal of Ru(bpy)₃Cl₂ using resonance energy transfer (RET) was established for ultra-sensitive determination of gibberellin (GA3). Au-doped g-C₃N₄ (g-C₃N₄/Au) was prepared on the surface of glassy carbon electrode, and then o-phenylenediamine was electropolymerized to achieve a molecularly imprinted polymer (MIP) film. The film was then eluted by methanol-acetic acid to obtain molecularly imprinted cavities for specifically recognizing GA3. Ru(bpy)₃²⁺ was used as probe and g-C₃N₄/Au was used as energy donor. The RET between Ru(bpy)₃²⁺ and g-C₃N₄/Au was used to enhance ECL intensity of Ru(bpy)₃²⁺. In addition, ECL-RET was combined with molecular imprinting technology to improve selectivity of the sensor. With the increase of the concentration of GA3, the molecularly imprinted cavities recombined with GA3, which made the ECL intensity gradually decreased. Under the optimized conditions, there was a linear relationship between ECL intensities and GA3 concentrations in the range from 4.00×10^-14 mol/L to 7.00×10^-11 mol/L, and the detection limit was 1.64×10^-14 mol/L (3S/K). The sensor was successfully applied to the detection of GA3 in beer, and the recoveries were 95.7%-103.7%.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

分子印迹传感器能量转移电化学发光法检测赤霉素

通讯作者: 李建平, likianping@263.net

English

A Molecularly Imprinted Electrochemical Luminescence Sensor for Detection of Gibberellin Based on Energy Transfer

Corresponding author: LI Jian-Ping, likianping@263.net

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

分子印迹传感器能量转移电化学发光法检测赤霉素

通讯作者: 李建平, likianping@263.net

English

A Molecularly Imprinted Electrochemical Luminescence Sensor for Detection of Gibberellin Based on Energy Transfer

Corresponding author: LI Jian-Ping, likianping@263.net

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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