激光剥蚀原位有机碳稳定同位素分析方法

引用本文: 张晗, 魏来, 廖旭, 王佳妮, 史秋月, 张娴. 激光剥蚀原位有机碳稳定同位素分析方法[J]. 分析化学, 2020, 48(6): 774-779. doi: 10.19756/j.issn.0253-3820.191769 shu

Citation: ZHANG Han, WEI Lai, LIAO Xu, WANG Jia-Ni, SHI Qiu-Yue, ZHANG Xian. In Situ Carbon Stable Isotope Analysis of Organic Carbon by Laser Ablation-Isotope Ratio Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2020, 48(6): 774-779. doi: 10.19756/j.issn.0253-3820.191769 shu

激光剥蚀原位有机碳稳定同位素分析方法

中国科学院城市环境研究所环境与健康重点实验室分析测试中心, 厦门 361021
基金项目:
本文系国家自然科学基金项目（No.41977210）资助

摘要: 自行研制了激光剥蚀取样与稳定同位素质谱联用接口，建立了以激光剥蚀微量取样的微区有机碳同位素分析方法。考察了捕集时间、样品量和激光能量等对同位素分馏的影响，采用3种标样验证了分析方法的准确性。结果表明，此装置可对固体样品进行高灵敏度的连续在线分析，样品用量仅10^-8 g，比传统的元素分析同位素质谱联用方法的样品用量降低了10⁴倍。本方法有效避免了激光剥蚀在取样、传输和离子化过程的多种分馏因素，获得了可重复的、高精度的测试结果。以¹³C标记的水稻为例，在叶尖获得了横截面的碳同位素分布差异信息。结合激光剥蚀技术原位取样的优势，此技术可用于考察固体样品微区高分辨的碳同位素分布，在环境、农林学、生态学领域具有良好的应用前景。

关键词:

English

In Situ Carbon Stable Isotope Analysis of Organic Carbon by Laser Ablation-Isotope Ratio Mass Spectrometry

Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
Received Date: 28 December 2019
Revised Date: 26 January 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 41977210).

Abstract: An interface of laser ablation and stable isotope mass spectrometry was developed for micro region organic carbon stable isotope analysis. Based on this, the¹³C/¹²C analytical method for organic carbon in standards and rice leaf based on laser ablation-isotope ratio mass spectrometry (LA-IRMS) was described. The results showed that this device could be used for the continuous on-line analysis of solid samples with high sensitivity, and the sampling amount was as low as tens of nanograms, which was 10⁴ times lower than that of traditional EA-IRMS analysis. This method effectively avoided many fractionation factors in the process of sampling, transmission and ionization, and obtained repeatable and high-precision test results. Combined with the advantages of laser ablation technology in situ sampling, this technique could obtain high-resolution carbon isotope distribution information of solid samples. This in-situ and on-line solid organic carbon isotope measurement technique was stable, reliable and accurate, and had a huge application prospect in the fields of environment, agriculture, forestry and ecology in the future.

Key words:

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激光剥蚀原位有机碳稳定同位素分析方法

English

In Situ Carbon Stable Isotope Analysis of Organic Carbon by Laser Ablation-Isotope Ratio Mass Spectrometry

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中国化学会秘书处

激光剥蚀原位有机碳稳定同位素分析方法

English

In Situ Carbon Stable Isotope Analysis of Organic Carbon by Laser Ablation-Isotope Ratio Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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