便携式血糖仪检测汞离子

引用本文: 石维平, 蔡杰, 杨雅妮, 罗欢欢, 刘冰倩, 付秋平. 便携式血糖仪检测汞离子[J]. 分析化学, 2019, 47(9): 1337-1343. doi: 10.19756/j.issn.0253-3820.191311 shu

Citation: SHI Wei-Ping, CAI Jie, YANG Ya-Ni, LUO Huan-Huan, LIU Bing-Qian, FU Qiu-Ping. Portable Glucose Meter for Detection of Mercury(Ⅱ) Ion[J]. Chinese Journal of Analytical Chemistry, 2019, 47(9): 1337-1343. doi: 10.19756/j.issn.0253-3820.191311 shu

便携式血糖仪检测汞离子

石维平 ^1, ,
蔡杰 ^1, ,
杨雅妮 ^1, ,
罗欢欢 ^1, ,
刘冰倩 ^1, ,
付秋平 ^2,

1.
贵州大学药学院, 贵州省合成药物工程实验室, 贵阳 550025;
2.
贵阳学院化学与材料工程学院, 贵阳 550025
基金项目:
本文系国家自然科学基金项目（Nos.21605029，21864007）、贵州省科技计划项目（黔科合平台人才[2017]5788）、贵州省科技计划项目（黔科合基础（2016）1032）、贵州大学引进人才科研项目（贵大人基合字（2015）40）和贵阳市财政支持贵阳学院学科与硕士点建设项目[HC-2019]资助

摘要: 利用T-Hg²⁺-T错配对汞离子（Hg²⁺）的特异性识别，以Fe₃O₄纳米磁珠（Fe₃O₄ nano-magnetic beads，MB）为反应平台，便携式血糖仪（Portable blood glucose meter，PGM）为检测手段，构建了一种简便灵敏的Hg²⁺检测方法。采用柠檬酸钠还原法制备金纳米颗粒（Gold nanoparticle，AuNPs），其表面用末端巯基化且富含胸腺嘧啶（T）的寡核苷酸探针（S1）和葡萄糖氧化酶（Glucose oxidase，GOx）修饰。在Hg²⁺存在下，功能化AuNPs通过T-Hg²⁺-T配对被捕获到富T探针（S2）修饰的MB上，AuNPs表面的GOx将葡萄糖（Glucose，Glu）氧化成葡萄糖酸和H₂O₂，使反应液中Glu含量降低，由PGM定量检测Glu含量。优化得到最佳实验条件：S2与GOx体积比为1：3，Hg²⁺反应时间为2 h，Glu水解时间为1 h。在最优条件下，Hg²⁺浓度在0.05~8.00 nmol/L范围内，体系PGM响应与的Hg²⁺浓度对数呈良好的线性关系，检出限为0.02 nmol/L （3σ）。实际水样中Hg²⁺的加标回收率为97.8%-113.7%，相对标准偏差为0.2%-1.3%，可以满足实际水样中Hg²⁺的检测要求。

关键词:

English

Portable Glucose Meter for Detection of Mercury(Ⅱ) Ion

1.
School of Pharmacy, Guizhou University, Synthetic Pharmaceutical Engineering Laboratory of Guizhou Province, Guiyang 550025, China;
2.
College of Chemistry and Materials Engineering, Guiyang University, Guiyang 550025, China
Received Date: 09 May 2019
Revised Date: 19 July 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21605029, 21864007), the Science and Technology Foundation of Guizhou Province ([2017]5788 Qian Ke He Platform for talents), the Science and Technology Foundation of Guizhou Province ([2016]1032 Qian Ke He Ji Chu), the Introduction of Talents Scientific Research Foundation of Guizhou University ([2015]40 Gui Da Ren Ji He Zi), the Discipline and Master's Site Construction Project of Guiyang University by Guiyang City Financial Support Guiyang University[HC-2019].

Abstract: Based on the specific recognition of T-Hg²⁺-T mismatched for mercury ions (Hg²⁺), a simple and sensitive method for detecting Hg²⁺ was developed by using Fe₃O₄ nano-magnetic beads (MB) as reaction platform and portable blood glucose meter (PGM) as detection means. Gold nanoparticles (AuNPs) were prepared by sodium citrate reduction. The surface of AuNPs was modified with sulfhydryl terminal-rich thymine (T) oligonucleotide probe (S1) and glucose oxidase (GOx). In the presence of Hg²⁺, functionalized AuNPs were captured by capture-probe (S2)-modified MB due to T-Hg²⁺-T coordination. The labeled GOx oxidized glucose (Glu) to gluconic acid and H₂O₂. Thus, the concentration of Glu in the reaction solution was reduced and could be detected quantitatively by PGM. The optimal conditions of the assay were as follows:the volume ratio of S2 and GOx was 1:3, the reaction time of Hg²⁺ was 2 h, and the hydrolysis time of Glu was 1 h. Under optimized conditions, the PGM response of the system had a good linear relationship with the logarithm of Hg²⁺ concentration. The linear range was 0.05-8.00 nmol/L, and the detection limit was 0.02 nmol/L (3σ). The recovery of Hg²⁺ in actual water samples was 97.8%-113.7%, and the relative standard deviation was 0.2%-1.3%, which met the detection requirements of Hg²⁺ in actual water samples.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

便携式血糖仪检测汞离子

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Portable Glucose Meter for Detection of Mercury(Ⅱ) Ion

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中国化学会秘书处

便携式血糖仪检测汞离子

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Portable Glucose Meter for Detection of Mercury(Ⅱ) Ion

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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