化学“101计划”综合化学实验教学案例设计——以MOC-16的激光共聚焦细胞成像为例

引用本文: 吴嘉仪, 王亚平, 江继军, 潘梅, 苏成勇. 化学“101计划”综合化学实验教学案例设计——以MOC-16的激光共聚焦细胞成像为例[J]. 大学化学, 2026, 41(6): 166-174. doi: 10.12461/PKU.DXHX202605044 shu

Citation: Jiayi Wu, Yaping Wang, Jijun Jiang, Mei Pan, Chengyong Su. Case design of the Chemistry “101 Plan” comprehensive chemistry experiment: taking laser confocal cell imaging of MOC-16 as an example[J]. University Chemistry, 2026, 41(6): 166-174. doi: 10.12461/PKU.DXHX202605044 shu

化学“101计划”综合化学实验教学案例设计——以MOC-16的激光共聚焦细胞成像为例

吴嘉仪 ^1, ,
王亚平 ^2, ,
江继军 ^1, ,
潘梅 ^{1,
,} ,
苏成勇 ^{1,
,}

1.
中山大学化学学院, 广东广州 510006;
2.
中山大学孙逸仙纪念医院, 广东广州 510030

通讯作者: 潘梅,Email:panm@mail.sysu.edu.cn; 苏成勇,Email:cesscy@mail.sysu.edu.cn

基金项目:
国家自然科学基金项目(92461302)

摘要: 面向化学“101计划”对综合化学实验课程“高阶性、创新性、挑战度”的要求，本文构建了一个以pH响应型有机金属超分子笼MOC-16的生物成像性能为核心、依托大型仪器共享平台的细胞成像综合实验。该实验涵盖了细胞培养、pH依赖性细胞膜靶向孵育、激光共聚焦单/双光子成像及图像分析等环节，形成了完整的科研训练模式，可系统培养学生操作大型仪器的实战能力与跨学科思维。作为化学“强基计划”现代化学研究方法与实验课程的核心模块，本实验已入选化学“101计划”合成化学实验教材综合部分，既可作为化学生物学实验单独开课，也可与超分子笼的合成与表征内容组合作为面向生物医药应用的综合化学实验开设，不仅凝练了契合拔尖创新人才培养目标的实验教学模式，也为大型仪器共享平台支撑基础学科人才培养提供了可复制、可推广的实践范例。

关键词:

English

Case design of the Chemistry “101 Plan” comprehensive chemistry experiment: taking laser confocal cell imaging of MOC-16 as an example

Jiayi Wu ^1, ,
Yaping Wang ^2, ,
Jijun Jiang ^1, ,
Mei Pan ^{1,
,} ,
Chengyong Su ^{1,
,}

1.
School of Chemistry, Sun Yat-sen University, Guangzhou 510006, Guangdong Province, China;
2.
Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510030, Guangdong Province, China

Corresponding author: Mei Pan, ; Chengyong Su,

Received Date: 13 May 2026
Revised Date: 21 May 2026
Available Online: 25 May 2026

Abstract: To meet the requirements of the Chemistry“101 Plan” for a “high-level, innovative, and challenging” comprehensive chemistry experiment course, this paper presents a comprehensive cell imaging experiment based on the biological imaging performance of pH-responsive organometallic supramolecular cage MOC-16 leveraging a large-scale instrument sharing platform. This experiment covers the complete scientific research chain including cell culture, pH-dependent cell membrane targeted incubation, one-/two-photon imaging via confocal laser scanning microscopy and image analysis, which can cultivate students’ practical ability to operate large-scale instruments and interdisciplinary thinking. As the core module of the “Modern Chemical Research Methods and Experiments” course under the chemistry “Strong Foundation Plan”, this experiment has been included in the comprehensive part of the synthetic chemistry experiment textbook of the Chemistry “101 Plan”. It can be offered as a standalone course in chemical biology experiments, or combined with the content of the synthesis and characterization of supramolecular cages to be offered as a comprehensive experiment for biomedical applications. It has refined an experimental teaching model that aligns with the goal of cultivating top-notch innovative talents and has also provided a replicable and scalable model for supporting the cultivation of basic disciplines through a large-scale instrument sharing platform.

Key words:

Chemistry “101 Plan”
/ Comprehensive chemistry experiment
/ Confocal laser scanning microscopy
/ Large-scale instrument sharing platform
/ Top-notch talent cultivation
/ Supramolecular materials

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化学“101计划”综合化学实验教学案例设计——以MOC-16的激光共聚焦细胞成像为例

通讯作者: 潘梅,Email:panm@mail.sysu.edu.cn; 苏成勇,Email:cesscy@mail.sysu.edu.cn

English

Case design of the Chemistry “101 Plan” comprehensive chemistry experiment: taking laser confocal cell imaging of MOC-16 as an example

Corresponding author: Mei Pan, ; Chengyong Su,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

化学“101计划”综合化学实验教学案例设计——以MOC-16的激光共聚焦细胞成像为例

通讯作者: 潘梅,Email:panm@mail.sysu.edu.cn; 苏成勇,Email:cesscy@mail.sysu.edu.cn

English

Case design of the Chemistry “101 Plan” comprehensive chemistry experiment: taking laser confocal cell imaging of MOC-16 as an example

Corresponding author: Mei Pan, ; Chengyong Su,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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