流式细胞仪：原理、应用与光谱技术的革新

引用本文: 彭建娜, 宋红杰, 张立春, 刘睿, 吕弋. 流式细胞仪：原理、应用与光谱技术的革新[J]. 大学化学, 2026, 41(4): 231-238. doi: 10.12461/PKU.DXHX202506070 shu

Citation: Jianna Peng, Hongjie Song, Lichun Zhang, Rui Liu, Yi Lü. Flow Cytometry: Principles, Applications, and Innovations in Spectroscopic Techniques[J]. University Chemistry, 2026, 41(4): 231-238. doi: 10.12461/PKU.DXHX202506070 shu

流式细胞仪：原理、应用与光谱技术的革新

彭建娜 ^1,2, ,
宋红杰 ^1, ,
张立春 ^1, ,
刘睿 ^{1,
,} ,
吕弋 ^1,3,

1.
四川大学化学学院, 四川成都 610064;
2.
云南大学化学科学与工程学院, 云南昆明 650091;
3.
四川大学分析测试中心, 四川成都 610064

通讯作者: 刘睿,E-mail:liur@scu.edu.cn

基金项目:
四川大学高等教育教学改革工程(第十一期)研究项目(SCU11230和SCU11232)

摘要: 流式细胞仪可以检测激光垂直照射细胞产生的散射光和荧光，从而完成细胞分析。光电技术进步推动它从单参数发展到多参数分析，并已经发展为全光谱流式细胞仪，能从复杂样品中获取大量生物信息，影响广泛。本文详细论述了流式细胞仪的发展历程、原理和多元应用，并重点探讨了光谱技术的革新，同时还指出未来发展方向。

关键词:

English

Flow Cytometry: Principles, Applications, and Innovations in Spectroscopic Techniques

1.
College of Chemistry, Sichuan University, Chengdu 610064, Sichuan Province, China;
2.
College of Chemical Science and Engineering, Yunnan University, Kunming 650091, Yunnan Province, China;
3.
Analytical & Testing Center, Sichuan University, Chengdu 610064, Sichuan Province, China

Corresponding author: Rui Liu, liur@scu.edu.cn

Received Date: 07 June 2025
Accepted Date: 11 July 2025
Available Online: 16 July 2025

Abstract: Flow cytometry enables the detection of scattered light and fluorescence emitted by cells when illuminated vertically by a laser beam, thereby facilitating comprehensive cellular analysis. The advancements in photoelectric technology have driven it from single-parameter to multi-parameter analysis, culminating in the development of full-spectrum flow cytometry. This evolution allows for the acquisition of extensive biological information from complex samples and has a wide range of influences. The article elaborates on the historical development, principles, and diverse applications of flow cytometry, and emphasizes the innovations in spectral technology. Additionally, it outlines potential future directions for the field.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

流式细胞仪：原理、应用与光谱技术的革新

通讯作者: 刘睿,E-mail:liur@scu.edu.cn

English

Flow Cytometry: Principles, Applications, and Innovations in Spectroscopic Techniques

Corresponding author: Rui Liu, liur@scu.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

流式细胞仪：原理、应用与光谱技术的革新

通讯作者: 刘睿,E-mail:liur@scu.edu.cn

English

Flow Cytometry: Principles, Applications, and Innovations in Spectroscopic Techniques

Corresponding author: Rui Liu, liur@scu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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