二次离子质谱-激光扫描共聚焦显微镜联用对单细胞显微成像-原理及应用

引用本文: 邵长芳, 赵耀, 吴魁, 贾菲菲, 罗群, 刘哲, 汪福意. 二次离子质谱-激光扫描共聚焦显微镜联用对单细胞显微成像-原理及应用[J]. 分析化学, 2018, 46(7): 1005-1016. doi: 10.11895/j.issn.0253-3820.181110 shu

Citation: SHAO Chang-Fang, ZHAO Yao, WU Kui, JIA Fei-Fei, LUO Qun, LIU Zhe, WANG Fu-Yi. Correlated Secondary Ion Mass Spectrometry-Laser Scanning Confocal Microscopy Imaging for Single Cell-Principles and Applications[J]. Chinese Journal of Analytical Chemistry, 2018, 46(7): 1005-1016. doi: 10.11895/j.issn.0253-3820.181110 shu

二次离子质谱-激光扫描共聚焦显微镜联用对单细胞显微成像-原理及应用

邵长芳 ^1,2, ,
赵耀 ^2, ,
吴魁 ^2, ,
贾菲菲 ^2, ,
罗群 ^2,3, ,
刘哲 ^1, ,
汪福意 ^2,3,

1.
曲阜师范大学抗癌药物开发与诊疗应用研究所, 山东省生命有机分析重点实验室, 山东省绿色天然产物及医药中间体开发重点实验室, 化学与化工学院, 曲阜师范大学, 曲阜 273165;
2.
北京分子科学国家实验室, 北京国家质谱中心, 活体分析化学中国科学院重点实验室, 中国科学院化学研究所, 北京 100190;
3.
中国科学院大学, 北京 100049
基金项目:
本文系国家自然科学基金项目（Nos.91543101，21671118，21790390，21790392，21575145，21621062）、泰山学者项目和中国科学院青年创新促进会（No.2017051）资助

摘要: 二次离子质谱（SIMS）分析主要用于半导体、地质等领域材料表面分析，随着科学仪器技术的发展，近年来，SIMS在生命科学领域中得到了越来越广泛的应用。SIMS可以实现对样品表面的质谱分析、化学成像以及深度剖析。三维SIMS成像分析的横向分辨率可达80~100 nm，纵向分辨率1~5 nm。但是，由于缺少特异性指示亚细胞结构的碎片离子，单细胞SIMS成像分析仍然面临着诸多挑战。激光扫描共聚焦显微成像（LSCM）作为一种单细胞成像技术已日趋成熟，可以对单细胞中的荧光分子或者对荧光标记的目标分子、细胞器成像，获得高分辨率亚细胞结构成像图。因而，LSCM在单细胞形貌分析上的优势和SIMS在单细胞化学成像方面的优势可以有效互补，其联合应用能显著提升单细胞分析的应用范围、深度和结果的准确性。本文重点介绍SIMS成像以及SIMS-LSCM联用成像在单细胞成像研究中的应用进展，在总结、评述该领域代表性工作的同时，对SIMS-LSCM联用成像在化学和生命科学研究，特别是在细胞生物学和药物发现领域的应用前景进行了展望。

关键词:

English

Correlated Secondary Ion Mass Spectrometry-Laser Scanning Confocal Microscopy Imaging for Single Cell-Principles and Applications

1.
Institute of Anticancer Agents Development and Theranostic Application, the Key Laboratory of Life-Organic Analysis and Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Department of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, China;
2.
Beijing National Laboratory for Molecular Sciences, National Centre for Mass Spectrometry in Beijing, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
3.
University of Chinese Academy of Sciences, Beijing 100049, China
Received Date: 09 February 2018
Revised Date: 23 April 2018
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 91543101, 21671118, 21790390, 21790392, 21575145, 21621062), the Taishan Scholars Program and the Youth Innovation Promotion Association of CAS (No. 2017051).

Abstract: Secondary ion mass spectrometry (SIMS) as a powerful surface analysis technique has been widely applied in semiconductor industry and geology research. Recently, with the development of instrumental technology, SIMS is attracting more and more attention in life sciences. SIMS can provide surface MS spectra, 2D/3D chemical images and depth profiling of substances simultaneously. The minimal lateral resolution of 2D SIMS imaging is 80 to 100 nm, and the longitudinal resolution of 3D SIMS imaging is about 1-5 nm. However, due to lack of specific ions to render the structures of organelles, SIMS imaging for single cells still has great challenges. Optical microscopy, in particular laser scanning confocal microscopy (LSCM), has been emerged to be an indispensable technique for single cell imaging and can obtain high spatial 2D/3D imaging to visualize the structures of organelles. Thus, the combinational use of SIMS and LSCM, which takes advantages of SIMS for molecular imaging and LSCM for morphological imaging, has greatly extended the application of SIMS imaging and ensured its accuracy at single cells level, providing novel insights into better understanding of the biological events inside cells. In this review, we focus on the development and application of SIMS imaging and the correlated SIMS and LSCM imaging in the research of cell biology and drug discovery. We anticipate that the combinational use of SIMS and LSCM imaging has promising future in biomedicine and life sciences.

Key words:

Secondary ion mass spectrometry
/ Laser scanning confocal microscopy
/ Correlated secondary ion mass spectrometry and laser scanning confocal microscopy imaging
/ Single cell imaging
/ Cell biology
/ Review

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

二次离子质谱-激光扫描共聚焦显微镜联用对单细胞显微成像-原理及应用

English

Correlated Secondary Ion Mass Spectrometry-Laser Scanning Confocal Microscopy Imaging for Single Cell-Principles and Applications

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

二次离子质谱-激光扫描共聚焦显微镜联用对单细胞显微成像-原理及应用

English

Correlated Secondary Ion Mass Spectrometry-Laser Scanning Confocal Microscopy Imaging for Single Cell-Principles and Applications

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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