Au纳米簇-WS<sub>2</sub>纳米片复合材料的合成及类过氧化物酶活性研究

引用本文: 王文俊, 吴燕虹, 林秀玲, 陈伟, 刘爱林, 彭花萍. Au纳米簇-WS₂纳米片复合材料的合成及类过氧化物酶活性研究[J]. 分析化学, 2018, 46(10): 1545-1551. doi: 10.11895/j.issn.0253-3820.181107 shu

Citation: WANG Wen-Jun, WU Yan-Hong, LIN Xiu-Ling, CHEN Wei, LIU Ai-Lin, PENG Hua-Ping. Synthesis of Au-WS₂ Nanocomposites and Study on Its Peroxidase Mimic Activity[J]. Chinese Journal of Analytical Chemistry, 2018, 46(10): 1545-1551. doi: 10.11895/j.issn.0253-3820.181107 shu

Au纳米簇-WS₂纳米片复合材料的合成及类过氧化物酶活性研究

1.
福建医科大学药学院药物分析系, 福州 350108;
2.
福建省高等学校纳米医药技术重点实验室, 福州 350108
基金项目:
本文系国家自然科学基金项目（No.21675024）、福建省科技创新联合资金项目（Nos.2016Y9054，2016Y9056）和福建省临床医学实验平台（附属第一医院）课题（No.FYKFKT-201707）资助

摘要: 通过静电组装技术制备了金纳米簇-硫化钨片纳米复合材料（Au-WS₂ NCs），此Au-WS₂NCs具有优良的过氧化物酶催化活性，能有效催化H₂O₂氧化3，3'，5，5'-四甲基联苯胺盐酸盐（TMB）发生显色反应。考察了Au-WS₂催化NCs/TMB/H₂O₂显色反应条件对此纳米复合材料类过氧化物酶活性的影响，并进行了稳态动力学分析。实验结果表明，Au-WS₂ NCs的酶催化反应遵循典型的Michaelis-Menten动力学模型。基于Au-WS₂ NCs在H₂O₂存在下对TMB的催化显色反应及葡萄糖在葡萄糖氧化酶作用下可产生H₂O₂的催化氧化反应，在最优实验条件下，实现了比色法测定H₂O₂和葡萄糖，检出限分别为6.0×10^-7 mol/L和3.1×10^-6 mol/L，并成功用于血糖的检测。

关键词:

English

Synthesis of Au-WS₂ Nanocomposites and Study on Its Peroxidase Mimic Activity

1.
Department of Pharmaceutical Analysis of the Fujian Medical University, Fuzhou 350004, China;
2.
The Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical Univeristy, Fuzhou 350004, China
Received Date: 09 February 2018
Revised Date: 30 August 2018
Fund Project:
This work was supported by the National Natural Science Foundation of China (No.21675024), the Joint Funds for the Innovation of Science and Technology, Fujian Province (Nos. 2016Y9054, 2016Y9056) and the Funds of Clinical Experiment Platform of Fujian Province, China (No. FYKFKT-201707)

Abstract: Gold nanoclusters-tungsten sulfide nanocomposites (Au-WS₂ NCs) were synthesized through electrostatic self-assembly method. The synthesized Au-WS₂ NCs could effectively catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂ due to its excellent peroxidase-like catalytic activity. The effects of Au-WS₂ NCs/TMB/H₂O₂ coloration conditions on peroxidase-like activity of Au-WS₂ NCs were investigated and the steady-state kinetic analysis was carried out. The experimental results showed that the Au-WS₂ NCs catalytic reaction using H₂O₂ and TMB as reaction substrates followed a typical Michaelis-Menten kinetic model. Based on the intrinsic peroxidase-like activity of Au-WS₂ NCs and that glucose could be oxidized by glucose oxidase to produce H₂O₂, a colorimetric detection method for H₂O₂ and glucose was established and the detection limits were 6.0×10^-7 mol/L and 3.1×10^-6 mol/L, respectively. Thus, a new colorimetric method was developed, which provided a significant reference value for other types of peroxidase mimics.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

Au纳米簇-WS₂纳米片复合材料的合成及类过氧化物酶活性研究

English

Synthesis of Au-WS₂ Nanocomposites and Study on Its Peroxidase Mimic Activity

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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中国化学会秘书处

Au纳米簇-WS2纳米片复合材料的合成及类过氧化物酶活性研究

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Synthesis of Au-WS2 Nanocomposites and Study on Its Peroxidase Mimic Activity

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

Au纳米簇-WS₂纳米片复合材料的合成及类过氧化物酶活性研究

Synthesis of Au-WS₂ Nanocomposites and Study on Its Peroxidase Mimic Activity

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs