离子液体-超声辅助-固相萃取法提取富集急性子药材中的4种凤仙萜四醇皂苷

引用本文: 吴谦, 林花, 刘迎, 金永日, 黎鹏, 刘伟杰, 李绪文. 离子液体-超声辅助-固相萃取法提取富集急性子药材中的4种凤仙萜四醇皂苷[J]. 分析化学, 2018, 46(6): 910-916. doi: 10.11895/j.issn.0253-3820.181062 shu

Citation: WU Qian, LIN Hua, LIU Ying, JIN Yong-Ri, LI Peng, LIU Wei-Jie, LI Xu-Wen. Ionic Liquid Ultrasonic-assisted Solid Phase Extraction of Four Kinds of Hosenkosides from Seeds of Impatiens balsamina L.[J]. Chinese Journal of Analytical Chemistry, 2018, 46(6): 910-916. doi: 10.11895/j.issn.0253-3820.181062 shu

离子液体-超声辅助-固相萃取法提取富集急性子药材中的4种凤仙萜四醇皂苷

吴谦 ^1, ,
林花 ^2, ,
刘迎 ^1, ,
金永日 ^1, ,
黎鹏 ^1, ,
刘伟杰 ^1, ,
李绪文 ^1,

1.
吉林大学化学学院, 长春 130012;
2.
吉林大学第一医院检验科, 长春 130012

摘要: 建立了一种绿色、快速、高效萃取和富集中药急性子中4种凤仙萜四醇皂苷的方法。采用离子液体并结合超声辅助萃取急性子中的化学成分，利用固相萃取小柱富集提取液中的凤仙萜四醇皂苷。通过考察离子液体种类及用量、pH值、超声时间、固相萃取剂和洗脱剂等实验条件对提取率的影响，确立了最优萃取剂为[C₆mim]Br，最优萃取条件：萃取剂用量为0.9 mL，提取液pH=7.0，超声为30 min，最优富集条件：固相萃取小柱填料为0.9 g CHP20/P120，上样流速为1.5 mL/min，洗脱剂为1.5 mL甲醇。在最优条件下，4种凤仙萜四醇苷加标回收率在92.1%-108.2%之间，相关系数范围为0.9945-0.9975，检出限范围为1.8~4.5 μg/mL，RSD<3.9%。本方法与传统回流提取方法提取效果接近，可准确测定急性子中4种凤仙萜四醇苷含量。

关键词:

English

Ionic Liquid Ultrasonic-assisted Solid Phase Extraction of Four Kinds of Hosenkosides from Seeds of Impatiens balsamina L.

WU Qian ^1, ,
LIN Hua ^2, ,
LIU Ying ^1, ,
JIN Yong-Ri ^1, ,
LI Peng ^1, ,
LIU Wei-Jie ^1, ,
LI Xu-Wen ^1,

1.
College of Chemistry, Jilin University, Changchun 130021, China;
2.
Clinical Laboratory of First Hospital, Jilin University, Changchun 130021, China
Received Date: 26 January 2018
Revised Date: 07 March 2018

Abstract: A green, rapid and efficient method was developed for the extraction of 4 kinds of hosenkosides from the seeds of Impatiens balsamina L. The hosenkosides were extracted by ionic liquid, and then enriched by solid phase extraction. The effects of the kinds and volume of ionic liquid, pH value, ultrasonic time, solid phase extractant and eluent on the extraction fields were investigated and optimized. Under the optimum conditions, the recoveries of 4 kinds of hosenkosides were between 92.1% and 108.2%, the correlation coefficients were 0.9945-0.9975, and the detection limits were 1.8-4.5 μg/mL. The RSD values were all less than 3.9%. The experimental results showed that this method was fast, efficient, environmental protection. This study provided a reference for the extraction and enrichment of trace components in Chinese medicinal herbs.

Key words:

1. [1]
  WANG Yu-Sheng. Chinese Pharmacological and Application. Beijing:People's Health Publishing House, 1983:799 王浴生. 中药药理与应用. 北京:人民卫生出版社, 1983:799
2. [2]
  ZHAO Liang-Qian. Jiangsu J. Tradit. Chin. Med., 2002, 23(9):25 赵良倩. 江苏中医药, 2002, 23(9):25
3. [3]
  MA You-Yun. Shanghai J. Tradit. Chin. Med., 2007, 41(1):78 马有运. 上海中医药杂志, 2007, 41(1):78
4. [4]
  Fu Y, Gao W, Yu J J, Chen J, Li H J, Li P. J. Pharmaceut. Biomed., 2012, 64-65(4):64-71
5. [5]
  WANG Wei, JU Cheng-Guo, ZHU Xu, ZHAO Huan-Jun. Chin. Tradit. Pat. Med., 2011, 33(2):199-202 王巍, 鞠成国, 朱旭, 赵焕君. 中成药, 2011, 33(2):199-202
6. [6]
  Wang Y C, Lin Y H. Fitoterapia. 2012, 83(8):1336-1344
7. [7]
  CHEN Ming-Xia, WANG Xiang-Li, ZHANG Yu-Jie. Chin. J. Chin. Mater. Med., 2006, 31(11):928-929 陈明霞, 王相立, 张玉杰. 中国中药杂志, 2006, 31(11):928-929
8. [8]
  TONG Miao-Miao, ZHAI Yan-Jun, WANG Tian-Min, LI Na. Chin. Tradit. Pat. Med. 2011, 33(7):1171-1174 佟苗苗, 翟延君, 王添敏, 李娜. 中成药, 2011, 33(7):1171-1174
9. [9]
  Chinese Pharmacopoeia Commission, Chinese Pharmacopoeia, vol. I, Beijing:The Medicine Science and Technology, 2015:263 中华人民共和国药典(一部). 国家药典委员会. 北京:中国医药科技出版社, 2015:263
10. [10]
  Wu Q, Jin Y R, Yao H, Liu Y, Li P, Li X W. Chem. Res. Chinese U., 2017, (5):1-5
11. [11]
  LI Hui-Jun, YU Jun-Jie, LI Ping. China Patent, 201010126336.4, 2012 李会军, 于俊杰, 李萍. 中国专利, 201010126336.4, 2012
12. [12]
  TAN Li-Na, LU Juan, ZHU Na, MENG Lei, ZHU Hong-Wei, LIU Yu-Wei, JIN Yong-Ri, LI Xu-Wen. Chin. J. Pharm. Anal., 2009, 29(5):717-720 谭丽娜, 陆娟, 朱娜, 孟磊, 朱红微, 刘玉玮, 金永日, 李绪文. 药物分析杂志, 2009, 29(5):717-720
13. [13]
  Li H J, Yu J J, Li P. J. Pharmaceut. Biomed., 2011, 54(4):674-680
14. [14]
  Sara L, Héctor R, Alberto A, Ana S. Fluid Phase Equilibr., 2014, 361(2):37-44
15. [15]
  Xu W, Chu K D, Li H, Zhang Y Q, Zheng H Y, Chen R L, Chen L D. Molecules, 2012, 17(12):14323-14335
16. [16]
  Ana F MCláudio, Ana M F, Mara G F, João A P C. Green Chem., 2013, 15(7):2002-2010
17. [17]
  Choi S A, Lee J S, Oh Y K, Jeong M J, Kim S W, Park J Y. Algal Res., 2014, 3(1):44-48
18. [18]
  YU Xiu-Pei, FENG Fang, TENG Bao-Qi, CHEN Yu-Yu. Pharm. Clin. Res., 2012, 20(3):264-266 于秀培, 冯芳, 滕宝褀, 陈玉宇. 药学与临床研究, 2012, 20(3):264-266
19. [19]
  Xu J J, Li Q, Cao J, Warner E, An M R, Tan Z J, Wang S L, Peng L Q, Liu X G. J. Chromatogr. A, 2016, 1463(9):32-41
20. [20]
  Yan H Y, Sun N, Han Y H, Yang C, Wang M Y, Wu R J. J. Chromatogr. A, 2013, 1307(17):21-26
21. [21]
  Visser A E, Swatloski R P, Reichert W M, Griffin S T, Rogers R D. Ind. Eng. Chem. Res., 2000, 39(10):3596-3604
22. [22]
  Wang X Z, Li X W, Li L J, Li M, Wu Q, Liu Y, Yang J, Jin Y R. Anal. Methods, 2016, 8(35):6566-6572
23. [23]
  Yang C, Sun Y Y, Kyung H R, Yan H Y. J. Chromatogr. A, 2014, 1361(26):53
24. [24]
  CHEN Yi-Xin, FENG Qing-Sheng, WANG Xiao-Zhong, LI Lan-Jie, LI Xu-Wen, JIN Yong-Ri. Chinese J. Anal. Chem., 2016, 44(7):1106-1111 陈一鑫, 冯清胜, 王晓中, 李兰杰, 李绪文, 金永日. 分析化学, 2016, 44(7):1106-1111
25. [25]
  Li H J, Liu Y, Yi Y T, Miao Q, Liu S J, Zhao F, Cong W, Wang C H, Xia C H. J. Chromatogr. B, 2017, 1048:56-63

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

离子液体-超声辅助-固相萃取法提取富集急性子药材中的4种凤仙萜四醇皂苷

English

Ionic Liquid Ultrasonic-assisted Solid Phase Extraction of Four Kinds of Hosenkosides from Seeds of Impatiens balsamina L.

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

离子液体-超声辅助-固相萃取法提取富集急性子药材中的4种凤仙萜四醇皂苷

English

Ionic Liquid Ultrasonic-assisted Solid Phase Extraction of Four Kinds of Hosenkosides from Seeds of Impatiens balsamina L.

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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