质子转移反应-飞行时间质谱检测呼出气体中痕量挥发性有机物

引用本文: 韩明聪, 董俊国, 彭真, 徐丽, 程平, 周振. 质子转移反应-飞行时间质谱检测呼出气体中痕量挥发性有机物[J]. 分析化学, 2018, 46(7): 1109-1115. doi: 10.11895/j.issn.0253-3820.171532 shu

Citation: HAN Ming-Cong, DONG Jun-Guo, PENG Zhen, XU Li, CHENG Ping, ZHOU Zhen. Proton Transfer Reaction Time-of-Flight Mass Spectrometry for Detection of Trace Volatile Organic Compounds in Breath[J]. Chinese Journal of Analytical Chemistry, 2018, 46(7): 1109-1115. doi: 10.11895/j.issn.0253-3820.171532 shu

质子转移反应-飞行时间质谱检测呼出气体中痕量挥发性有机物

韩明聪 ^1, ,
董俊国 ^1, ,
彭真 ^2, ,
徐丽 ^1, ,
程平 ^1, ,
周振 ^2,

1.
上海大学环境与化学工程学院, 上海 200444;
2.
昆山禾信质谱技术有限公司, 昆山 215133
基金项目:
本文系国家重大科学仪器设备开发专项（No.2011YQ170067）资助

摘要: 通过对自主研制的质子转移反应飞行时间质谱（PTR-TOF-MS）进样口的改造，实现了对呼出气体的实时、在线定量检测，并对23名志愿者（11名男性健康受试者、11名女性健康受试者和1名胃病患者）的呼出气体进行28天的实时在线监测。结果表明，健康人群呼出气体中主要的挥发性有机物（VOCs）为甲醛、丙烯、乙醛、丙酮、异丙醇和异戊二烯等，浓度基本服从对数正态分布，且甲醛、丙烯、异丙醇主要集中在40~100 ppb浓度范围内，乙醛的浓度主要集中在80~180 ppb浓度范围内，丙酮主要集中在500~1500 ppb浓度范围内，异戊二烯主要集中在8~20 ppb的浓度范围内；呼出气体浓度在性别上也存在差异，其中，受试人群中男性呼出气体中丙酮的浓度高于女性，而乙醛和异丙醇浓度低于女性。胃病患者的呼出气体中甲醛和丙酮含量明显高于健康人群；乙醇和乙醛是饮酒者呼出气体的主要标志物，乙醛是乙醇的代谢产物，随着乙醇浓度的变化而变化。

关键词:

English

Proton Transfer Reaction Time-of-Flight Mass Spectrometry for Detection of Trace Volatile Organic Compounds in Breath

1.
School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;
2.
Kunshan Hexin Mass Spectrometry Co, Ltd., Kunshan 215133, China
Received Date: 28 December 2017
Revised Date: 28 April 2018
Fund Project:
This work was supported by the National Instrumentation Program (No. 2011YQ170067).

Abstract: Based on the modification of inlet of a proton transfer reaction time of flight mass spectrometry (PTR-TOF-MS) instrument developed in our laboratory, a new method for real-time and on-line quantitation of volatile organic compounds (VOCs) from human exhalation was established. A 28-day real-time and on-line monitoring of exhaled breath from 23 volunteers (11 male healthy subjects, 11 female healthy subjects and 1 stomach-sick patient) was carried out and the experimental results showed that the major potential VOCs markers were identified as formaldehyde, propylene, acetone, acetaldehyde, isopropanol and isoprene, and their concentrations obeyed the Normal Distribution. The concentrations of formaldehyde, propylene and isopropanol were mainly in the range of 40 to 100 ppb, acetaldehyde in the range of 80 to 180 ppb, acetone in the range of 500 to 1500 ppb, and isoprene in the range of 8 to 20 ppb. Meanwhile, the concentrations for some compounds were different for men and women. Men have higher level of acetone, and women have higher levels of acetaldehyde and isopropanol. In addition, the concentrations of formaldehyde and acetone in the exhaled breath of stomach sicknesses were significantly higher than that in healthy people. Ethanol and acetaldehyde were the main potential markers of exhale breath after drinking alcohol. The acetaldehyde was the major metabolite of ethanol, and the concentration of acetaldehyde changed with the concentration variation of ethanol in degradation process.

Key words:

Breath analysis
/ Proton transfer reaction time-of-flight mass spectrometry
/ Exhale markers
/ Concentration distribution.

1. [1]
  Boots A W, vanBerkel J J B N, Dallinga J W, Smolinska A, Wouters E F, van Schooten F J. J. Breath Res., 2012, 6(2):027108
2. [2]
  Sun X H, Shao K, Wang T. Anal. Bioanal. Chem., 2016, 408(11):2759-2780
3. [3]
  Kurada S, Alkhouri N, Fiocchi C, Dweik R, Rieder F. Aliment. Pharmacol. Ther., 2015, 41(4):329-341
4. [4]
  Chan H P, Lewis C, Thomas P S.Lung Cancer, 2009, 63(2):164-168
5. [5]
  Williams J, Pleil J. J. Breath Res., 2016, 10(3):032001
6. [6]
  Cao W Q, Duan Y X. Crit. Rev. Anal. Chem., 2007, 37(1):3-13
7. [7]
  Yokokawa T, Sato T, Suzuki S, Oikawa M, Yoshihisa A, Kobayashi A, Yamaki T, Kunii H, Nakazato K, Suzuki H, Saitoh S, Ishida T, Shimouchi A, Takeishi Y. BMC Cardiovasc. Disor., 2017, 17(1):280
8. [8]
  Davies S J, Spanẽl P, Smith D. Bioanalysis, 2014, 6(6):843-857
9. [9]
  Rosias P P, Dompeling E, Dentener M A, Pennings H J, Hendriks H J E, van Lersel M P A, Jobsis Q. Pediatr. Pulmonol., 2004, 38(2):107-114
10. [10]
  Graham D Y, Malaty H M, Cole R A, Martin R F, Klein P D. Am. J. Gastroenterol., 2001, 96(6):1741-1745
11. [11]
  Sanchez J M, Sacks R D. Anal. Chem., 2003, 75(10):2231-2236
12. [12]
  Rodionov A A, Revelsky A I, Revelsky I A, Anokhina T N, Anaev E K. J. Anal. Chem., 2014, 69(14):1330-1336
13. [13]
  Wilson A D. Metabolites, 2015, 5(1):140-163
14. [14]
  Ross B M, Babgi R. J. Breath Res., 2017, 11(4):046001
15. [15]
  Wang Y, Jiang J C, Hua L, Hou K Y, Xie Y Y, Chen P, Liu W, Li Q Y, Wang S, Li H Y. Anal. Chem., 2016, 88(18):9047-9055
16. [16]
  Zhan X, Duan J, Duan Y. Mass Spectrom. Rev., 2013, 32(2):143-165
17. [17]
  WU Xiao-Mei, PENG Zhen, DONG Jun-Guo, GAO Wei, HUANG Zheng-Xu, CHENG Ping, ZHOU Zhen. Journal of Chinese Mass Spectrometry Society, 2015, 36(1):1-7 伍小梅, 彭真, 董俊国, 高伟, 黄正旭, 程平, 周振. 质谱学报, 2015, 36(1), 1-7
18. [18]
  Dewan I, Rao B L S P. Ann. Inst. Statist. Math., 2003, 55(1):111-119
19. [19]
  SHEN Cheng-Yin, LI Jian-Quan, WANG Hong-Zhi, ZHI Zhong-Hua, WANG Hong-Mei, HUANG Chao-Qun, LIU Sheng, JIANG Hai-He, CHU Yan-Nan. Chinese J. Anal. Chem., 2012, 40(5):773-777 沈成银, 李建权, 王宏志, 志中华, 王鸿梅, 黄超群, 刘升, 江海河, 储焰南. 分析化学, 2012, 40(5):773-777
20. [20]
  Bajtarevic A, Ager C, Pienz M, Klieber M, Schwarz K, Ligor M, Ligor T, Filipiak W, Denz H, Fiegl M, Hilbe W, Weiss W, Lukas P, Jamnig H, Hackl M, Haidenberger A, Buszewski B, Miekisch W, Schubert J, Amann A. BMC Cancer, 2009, 9(1):348
21. [21]
  Schwoebel H, Schubert R, Sklorz M, Kischkel S, Zimmermann R, Schubert J K, Miekisch W. Anal. Bioanal. Chem., 2011, 401(7):2079-2091
22. [22]
  Turner C, panẽl P, Smith D. Physiol. Meas., 2006, 27(4):321-337
23. [23]
  Kushch I, Arendacká B, Štolc S, Mochalski P, Filipiak W, Schwarz K, Schwentner L, Schmid A, Dzien A, Lechleiner M, Witkovsky V, Miekisch W, Schubert J, Unterkofler K, Amann A. Clin. Chem. Lab. Med., 2008, 46(7):1011-1018
24. [24]
  Chen Y S, Zhang Y X, Pan F, Liu J, Wang K, Zhang C L, Cheng S L, Lu L G, Zhang W, Zhang Z, Zhi X, Zhang Q, Alfranca G, Fuente J M, Chen D, Cui D X. ACS Nano, 2016, 10(9):8169-8179
25. [25]
  Sémon E, Arvisenet G, Guichard E, Le Quéré J L. J. Mass Spectrom., 2018, 53(1):65-77
26. [26]
  Smith D, Wang T, Španěl P. Physiol. Meas., 2002, 23(3):477-489
27. [27]
  Hlastala M P, Anderson J C. Ann. Biomed. Eng., 2007, 35(2):264-272

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质子转移反应-飞行时间质谱检测呼出气体中痕量挥发性有机物

English

Proton Transfer Reaction Time-of-Flight Mass Spectrometry for Detection of Trace Volatile Organic Compounds in Breath

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中国化学会秘书处

质子转移反应-飞行时间质谱检测呼出气体中痕量挥发性有机物

English

Proton Transfer Reaction Time-of-Flight Mass Spectrometry for Detection of Trace Volatile Organic Compounds in Breath

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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