基于Ru(bpy)<sub>3</sub><sup>2+</sup>/AuNPs/SWCNTs/腺苷适配体电化学发光生物传感器用于腺苷的检测

引用本文: 樊雪梅, 王书民, 李哲建, 贾雪艳, 郑行望. 基于Ru(bpy)₃²⁺/AuNPs/SWCNTs/腺苷适配体电化学发光生物传感器用于腺苷的检测[J]. 分析化学, 2017, 45(9): 1353-1359. doi: 10.11895/j.issn.0253-3820.170216 shu

Citation: FAN Xue-Mei, WANG Shu-Min, LI Zhe-Jian, JIA Xue-Yan, ZHENG Xing-Wang. Determination of Adenosine Based on Ru(bpy)₃²⁺/AuNPs/SWCNTs/Adenosine Aptamer Electrochemiluminescence Sensor[J]. Chinese Journal of Analytical Chemistry, 2017, 45(9): 1353-1359. doi: 10.11895/j.issn.0253-3820.170216 shu

基于Ru(bpy)₃²⁺/AuNPs/SWCNTs/腺苷适配体电化学发光生物传感器用于腺苷的检测

1.
商洛学院化学工程与现代材料学院/陕西省尾矿资源综合利用重点实验室, 商洛 726000;2 西北师范大学化学化工学院, 兰州 730070;3 陕西师范大学化学化工学院, 西安 710062
基金项目:
本文系国家自然科学基金项目（No.30970696）、陕西省科技统筹创新工程计划项目（No.2012KTDZ02-02）、陕西省教育厅项目（No.16JS028）、商洛市科技局项目（Nos.SK-2014-1，SK2015-37）和商洛学院项目（No.15sky024）资助

摘要: 利用AuNPs/Nafion复合膜技术固定Ru（bpy）₃²⁺，采用羧基化碳纳米管固定氨基化腺苷适配体，制备腺甘电化学发光生物传感器。采用循环伏安法和电化学发光法对传感器进行表征。结果表明，此传感器具有良好的稳定性和重现性。腺苷与传感器作用后，腺苷与其适配体形成G四面体结构，Ru（bpy）₃²⁺的电化学发光强度降低。在最佳实验条件下，电化学发光强度降低量与腺苷浓度的负对数在1.0×10^-11~1.0×10^-7 mol/L范围内呈良好的线性关系，线性方程为ΔI_ECL=-890lgC-5050，检出限（S/N=3）为5.0×10^-12 mol/L。对1.0×10^-10 mol/L腺苷平行测定11次，相对标准偏差为2.7%。用于尿液中腺苷的测定，加标回收率在97.1%-110.0%之间。

关键词:

English

Determination of Adenosine Based on Ru(bpy)₃²⁺/AuNPs/SWCNTs/Adenosine Aptamer Electrochemiluminescence Sensor

1.
College of Chemical Engineering and Modern Materials, Shangluo University, Shangluo 726000, China;2 School of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China;3 School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China
Received Date: 05 April 2017
Revised Date: 14 June 2017
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 30970696).

Abstract: Based on the AuNPs/Nafion composite membrane technology and immobilization of amino adenosine aptamer using carboxyl carbon nanotubes on the surface of a glassy carbon electrode, a electrochemiluminescence sensor was preparated. The sensor was characterized by cyclic voltammetry and electrochemical luminescence. The result showed that the sensor had a good stability and reproducibility. Adenosine and adenosine aptamer could form G-tetrahedral structure, leading a decrease of ECL intensity. Under the optimum experimental conditions, the relative ECL intensity showed a good linear relationship to the negative logarithm of adenosine concentration in the range of 1.0×10^-11-1.0×10^-7 mol/L, the linear equations was ΔI_ECL=-890lgC-5050 with a detection limit of 5.0×10^-12 mol/L. The RSD was 2.7% in 11 times measurement of adenosine (1.0×10^-10 mol/L). The recovery was 97.1%-110.0% in the determination of real adenosine sample.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于Ru(bpy)₃²⁺/AuNPs/SWCNTs/腺苷适配体电化学发光生物传感器用于腺苷的检测

English

Determination of Adenosine Based on Ru(bpy)₃²⁺/AuNPs/SWCNTs/Adenosine Aptamer Electrochemiluminescence Sensor

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基于Ru(bpy)32+/AuNPs/SWCNTs/腺苷适配体电化学发光生物传感器用于腺苷的检测

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Determination of Adenosine Based on Ru(bpy)32+/AuNPs/SWCNTs/Adenosine Aptamer Electrochemiluminescence Sensor

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于Ru(bpy)₃²⁺/AuNPs/SWCNTs/腺苷适配体电化学发光生物传感器用于腺苷的检测

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