C<sub>18</sub>柱串联冠醚手性柱高效分离3种芳香族氨基酸对映体

引用本文: 刘育坚, 刘智敏, 许志刚. C₁₈柱串联冠醚手性柱高效分离3种芳香族氨基酸对映体[J]. 分析化学, 2017, 45(3): 369-373. doi: 10.11895/j.issn.0253-3820.160731 shu

Citation: LIU Yu-Jian, LIU Zhi-Min, XU Zhi-Gang. Enantiomeric Separation of Three Kinds of Aromatic Amino Acids by C₁₈ Column Tandem Crown Ether Chiral Column[J]. Chinese Journal of Analytical Chemistry, 2017, 45(3): 369-373. doi: 10.11895/j.issn.0253-3820.160731 shu

C₁₈柱串联冠醚手性柱高效分离3种芳香族氨基酸对映体

昆明理工大学理学院, 昆明 650500
基金项目:
本文系国家自然科学基金(No.21565018)和昆明理工大学校级学科方向团队项目(No.14078356)资助

摘要: 将C₁₈柱与手性冠醚柱串联，建立了一种反相高效液相色谱法用于3种芳香族氨基酸对映体同时拆分的方法。考察了反相色谱流动相的组成、pH值、柱温、流速对对映体拆分的影响。实验结果表明，当流动相为HClO₄-乙腈溶液(86：14，V/V，pH 2.0)、柱温20℃、流速0.4 mL/min时，3种氨基酸对映体可获得基线分离。进一步对比了C₁₈柱、冠醚手性柱和串联顺序不同的4种分离模式，结果表明，C₁₈柱不能拆分氨基酸对映体，仅能分离不同种类氨基酸；冠醚手性柱可分离氨基酸映体，但不同种类氨基酸色谱峰出现重叠；串联模式能实现3种氨基酸对映体的基线分离，实现双柱优势互补，而串联顺序对分离影响不大，仅影响色谱峰的峰形。

关键词:

English

Enantiomeric Separation of Three Kinds of Aromatic Amino Acids by C₁₈ Column Tandem Crown Ether Chiral Column

Faculty of Science, Kunming University of Science and Technology, Kunming 650500, China
Received Date: 28 September 2016
Revised Date: 12 December 2016
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 21565018)

Abstract: A reversed phase high performance liquid chromatographic (RP-HPLC) method was proposed by using C₁₈ column tandem crown ether chiral column for simultaneous separation of three kinds of aromatic amino acid enantiomers. The chromatographic conditions, including mobile phase proportion, pH, column temperature and flow rate, were optimized. The experimental results showed that the three aromatic amino acid enantiomers could be successfully separated under the optimal conditions such as perchloric acid solution-acetonitrile (86:14, V/V, pH=2) as mobile phase, column temperature of 20℃ and flow rate of 0.4 mL/min. The separation under four HPLC column connection modes was further investigated. The results revealed that different kinds of amino acids could be separated on C₁₈ column, but not for amino acid enantiomers; amino acid enantiomers could be separated on crown ether chiral column, but the chromatographic peaks of each amino acid enantiomers overlapped seriously; baseline separation of three amino acid enantiomers could be obtained under the connection of two columns, but the separation under different column connection sequence almost had no difference except the peak shape.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

C₁₈柱串联冠醚手性柱高效分离3种芳香族氨基酸对映体

English

Enantiomeric Separation of Three Kinds of Aromatic Amino Acids by C₁₈ Column Tandem Crown Ether Chiral Column

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中国化学会秘书处

C18柱串联冠醚手性柱高效分离3种芳香族氨基酸对映体

English

Enantiomeric Separation of Three Kinds of Aromatic Amino Acids by C18 Column Tandem Crown Ether Chiral Column

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

C₁₈柱串联冠醚手性柱高效分离3种芳香族氨基酸对映体

Enantiomeric Separation of Three Kinds of Aromatic Amino Acids by C₁₈ Column Tandem Crown Ether Chiral Column

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs