中空纳米二氧化硅复合微球的制备及其对蛋白的亲和分离

田淑芳 邹雪艳 卢海涛 何建英 陈丹云 赵彦保 郭静玉

引用本文: 田淑芳, 邹雪艳, 卢海涛, 何建英, 陈丹云, 赵彦保, 郭静玉. 中空纳米二氧化硅复合微球的制备及其对蛋白的亲和分离[J]. 无机化学学报, 2015, 31(7): 1329-1334. doi: 10.11862/CJIC.2015.191 shu
Citation:  TIAN Shu-Fang, ZOU Xue-Yan, LU Hai-Tao, HE Jian-Ying, CHEN Dan-Yun, ZHAO Yan-Bao, GUO Jing-Yu. Synthesis of Nanometer Hollow Silica Composite Microspheres for Affinity Separation of Protein[J]. Chinese Journal of Inorganic Chemistry, 2015, 31(7): 1329-1334. doi: 10.11862/CJIC.2015.191 shu

中空纳米二氧化硅复合微球的制备及其对蛋白的亲和分离

    通讯作者: 邹雪艳E-mail:zouxy182838@163.com
  • 基金项目:

    河南省教育厅科学技术重点研究项目(No.14B150003) (No.14B150003)

    国家自然科学基金(No.21271062)资助项目。 (No.21271062)

摘要: 利用水热法合成了中空巯基纳米二氧化硅微球(SiO2-SH), 然后在其表面修饰亚氨基二乙酸基团(-IDA), 形成了中空SiO2-SH/IDA双功能化纳米微球。利用该纳米微球表面的-SH和-IDA双功能团, 可以更多的吸附溶液中的Ni2+, 形成SiO2-SH/IDA-Ni2+复合微球从而可以更好的分离以六聚组氨酸为标签的(His-tagged)蛋白。结果显示制备的样品对分离His-tagged蛋白具有广谱性, 并且具有较好的再生能力。

English

  • 
    1. [1] Nanguo L, Bradley S P, Victor I K. J. Am. Chem. Soc., 2006, 128:15362-15363[1] Nanguo L, Bradley S P, Victor I K. J. Am. Chem. Soc., 2006, 128:15362-15363

    2. [2] Li X L, Liu Y Q, Fu L, et al. J. Phys. Chem. C, 2007, 111: 7661-7665[2] Li X L, Liu Y Q, Fu L, et al. J. Phys. Chem. C, 2007, 111: 7661-7665

    3. [3] Han J, Song G P, Guo R. Chem. Mater., 2007, 19:973-975[3] Han J, Song G P, Guo R. Chem. Mater., 2007, 19:973-975

    4. [4] Joo S H, Ryoo R, Kruk M, et al. J. Phys. Chem., 2002, 106: 4640-4646[4] Joo S H, Ryoo R, Kruk M, et al. J. Phys. Chem., 2002, 106: 4640-4646

    5. [5] Yamada T, Zhou H S, Hiroishi D, et al. Adv. Mater., 2003, 15:511-513[5] Yamada T, Zhou H S, Hiroishi D, et al. Adv. Mater., 2003, 15:511-513

    6. [6] Yonezawa T, Matsune H, Kimizuka N. Adv. Mater., 2003, 15: 499-503[6] Yonezawa T, Matsune H, Kimizuka N. Adv. Mater., 2003, 15: 499-503

    7. [7] Feng X M, Mao C J, Yang G, et al. Langmuir, 2006, 22:4384-4389[7] Feng X M, Mao C J, Yang G, et al. Langmuir, 2006, 22:4384-4389

    8. [8] Li L L, Chu Y, Liu Y, et al. J. Phys. Chem., 2007, 111:2123-2127[8] Li L L, Chu Y, Liu Y, et al. J. Phys. Chem., 2007, 111:2123-2127

    9. [9] Masahiro F, Kumi S, Ikuko S, et al. Nano Lett., 2006, 6:2925-2928[9] Masahiro F, Kumi S, Ikuko S, et al. Nano Lett., 2006, 6:2925-2928

    10. [10] Melero J A, Rafael G, Gabriel M, et al. Energy Fuels, 2007, 21:1782-1791[10] Melero J A, Rafael G, Gabriel M, et al. Energy Fuels, 2007, 21:1782-1791

    11. [11] Ji X, Hu Q, Hampsey J E, et al. Chem. Mater., 2006, 18: 2265-2274[11] Ji X, Hu Q, Hampsey J E, et al. Chem. Mater., 2006, 18: 2265-2274

    12. [12] Yang D, Xu Y, Wu D, et al. J. Phys. Chem. C, 2007, 111: 999-1004[12] Yang D, Xu Y, Wu D, et al. J. Phys. Chem. C, 2007, 111: 999-1004

    13. [13] Xie H Y, Zhen R, Wang B, et al. J. Phy. Chem. C, 2010, 114: 4825-4830[13] Xie H Y, Zhen R, Wang B, et al. J. Phy. Chem. C, 2010, 114: 4825-4830

    14. [14] Yang L, Guo C, Chen S, et al. Ind. Eng. Chem. Res., 2009, 48:944-950[14] Yang L, Guo C, Chen S, et al. Ind. Eng. Chem. Res., 2009, 48:944-950

    15. [15] Lee I S, Lee N, Park J, et al. J. Am. Chem. Soc., 2006, 128: 10658-10659[15] Lee I S, Lee N, Park J, et al. J. Am. Chem. Soc., 2006, 128: 10658-10659

    16. [16] Lee K S, Lee I S. Chem. Commun., 2008:709-711[16] Lee K S, Lee I S. Chem. Commun., 2008:709-711

    17. [17] Porath J, Carlsson J, Olsson I, et al. Nature, 1975, 258:598-599[17] Porath J, Carlsson J, Olsson I, et al. Nature, 1975, 258:598-599

    18. [18] Xu F, Geiger J H, Baker G L, et al. Langmuir, 2011, 27: 3106-3112[18] Xu F, Geiger J H, Baker G L, et al. Langmuir, 2011, 27: 3106-3112

    19. [19] Ahrends R, Pieper S, Neumann B, et al. Anal. Chem., 2009, 81:2176-2184[19] Ahrends R, Pieper S, Neumann B, et al. Anal. Chem., 2009, 81:2176-2184

    20. [20] Kim J, Piao Y, Lee N, et al. Adv. Mater., 2009, 22:57-60[20] Kim J, Piao Y, Lee N, et al. Adv. Mater., 2009, 22:57-60

    21. [21] Jeng M F, Campbell A P, Begley T, et al. Structure, 1994, 2: 853-868[21] Jeng M F, Campbell A P, Begley T, et al. Structure, 1994, 2: 853-868

    22. [22] LaVallie E R, Rehemtulla A, Racie L A, et al. J. Biol. Chem., 1993, 268:23311-23317[22] LaVallie E R, Rehemtulla A, Racie L A, et al. J. Biol. Chem., 1993, 268:23311-23317

    23. [23] ZOU Xue-Yan(邹雪艳), CHU Liu-Jie(褚留杰), DONG Shuo (董烁), et al. Chem. J. Chinese Universities(高等学校化学学报), 2012, 33(7):1394-1400[23] ZOU Xue-Yan(邹雪艳), CHU Liu-Jie(褚留杰), DONG Shuo (董烁), et al. Chem. J. Chinese Universities(高等学校化学学报), 2012, 33(7):1394-1400

    24. [24] Li B J, Zou X Y, Zhao Y B, et al. Mater. Sci. Eng. C, 2013, 33:2595-2600[24] Li B J, Zou X Y, Zhao Y B, et al. Mater. Sci. Eng. C, 2013, 33:2595-2600

    25. [25] Zou X Y, Li K, Yin Y B, et al. Mater. Sci. Eng. C, 2014, 34: 468-473[25] Zou X Y, Li K, Yin Y B, et al. Mater. Sci. Eng. C, 2014, 34: 468-473

    26. [26] YAN Hong(颜红), WANG Chong(王冲), ZHOU Xiao-Hui(周小会), et al. Chinese J. Inorg. Chem.(无机化学学报), 2011, 27:1642-1648[26] YAN Hong(颜红), WANG Chong(王冲), ZHOU Xiao-Hui(周小会), et al. Chinese J. Inorg. Chem.(无机化学学报), 2011, 27:1642-1648

    27. [27] Ma Z Y, Liu X Q, GuanY P, et al. Colloid Surf. A, 2006, 275:87-91[27] Ma Z Y, Liu X Q, GuanY P, et al. Colloid Surf. A, 2006, 275:87-91

    28. [28] SUN Yong-Liang(孙永亮), LI Shu-Juan(李淑娟), HU Dao-Dao(胡道道), et al. J. Shaanxi Normal University(陕西师范大学学报), 2007, 35:67-71[28] SUN Yong-Liang(孙永亮), LI Shu-Juan(李淑娟), HU Dao-Dao(胡道道), et al. J. Shaanxi Normal University(陕西师范大学学报), 2007, 35:67-71

    29. [29] Xie H Y, Zheng R, Wang B, et al. J. Phys. Chem. C, 2010, 114:4825-4830[29] Xie H Y, Zheng R, Wang B, et al. J. Phys. Chem. C, 2010, 114:4825-4830

  • 加载中
计量
  • PDF下载量:  0
  • 文章访问数:  296
  • HTML全文浏览量:  52
文章相关
  • 收稿日期:  2015-01-24
  • 网络出版日期:  2015-04-25
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

/

返回文章