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中空纳米二氧化硅复合微球的制备及其对蛋白的亲和分离

田淑芳 ,  邹雪艳 ,  卢海涛 ,  何建英 ,  陈丹云 ,  赵彦保 ,  郭静玉

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引用本文: 田淑芳, 邹雪艳, 卢海涛, 何建英, 陈丹云, 赵彦保, 郭静玉. 中空纳米二氧化硅复合微球的制备及其对蛋白的亲和分离[J]. 无机化学学报, 2015, 31(7): 1329-1334. doi: 10.11862/CJIC.2015.191 shu
Citation:  TIAN Shu-Fang, ZOU Xue-Yan, LU Hai-Tao, HE Jian-Ying, CHEN Dan-Yun, ZHAO Yan-Bao, GUO Jing-Yu. Synthesis of Nanometer Hollow Silica Composite Microspheres for Affinity Separation of Protein[J]. Chinese Journal of Inorganic Chemistry, 2015, 31(7): 1329-1334. doi: 10.11862/CJIC.2015.191 shu

中空纳米二氧化硅复合微球的制备及其对蛋白的亲和分离

  • 1.

    1 河南大学化学化工学院, 开封 475004;

  • 2.

    2 河南大学特种功能材料重点实验室, 开封 475004;

  • 3.

    3 河南大学民生学院, 开封 475004

    • 通讯作者: 邹雪艳E-mail:zouxy182838@163.com
  • 基金项目:

    河南省教育厅科学技术重点研究项目(No.14B150003) (No.14B150003)

    国家自然科学基金(No.21271062)资助项目。 (No.21271062)

摘要: 利用水热法合成了中空巯基纳米二氧化硅微球(SiO2-SH), 然后在其表面修饰亚氨基二乙酸基团(-IDA), 形成了中空SiO2-SH/IDA双功能化纳米微球。利用该纳米微球表面的-SH和-IDA双功能团, 可以更多的吸附溶液中的Ni2+, 形成SiO2-SH/IDA-Ni2+复合微球从而可以更好的分离以六聚组氨酸为标签的(His-tagged)蛋白。结果显示制备的样品对分离His-tagged蛋白具有广谱性, 并且具有较好的再生能力。

English

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  • 收稿日期:  2015-01-24
  • 网络出版日期:  2015-04-25
通讯作者: 陈斌, bchen63@163.com
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