摘要: The quantitative detection of biological metabolites is a crucial route for early diagnosis of human diseases. Exhaled ammonia (NH3), originating from abnormal metabolism, is normally recognized as the biomarker for liver and kidney lesions. Therefore, developing highly sensitive fluorescent sensing materials is expected to replace the traditional clinical blood tests and facilitate painless diagnosis and telemedicine for patients. However, the weak interaction for ammonia and the small color switching range of fluorescence sensors become the most pressing problem at present. Herein, a porphyrin-based hydrogen-bonded organic framework (HOF-6) with abundant supermolecule interactions in the confined pore space is developed for highly sensitive ammonia detection. The strong interactions between ammonia and the framework greatly promote the electron rearrangement and enhance the intensity of fluorescence, enabling HOF-6 to successfully achieve trace amounts of ammonia sensing with the limit detection of 0.2 ppm. With the ultrahigh selectivity for ammonia, HOF-6 can accurately determine the amount of ammonia in breath of patients, and the test results are highly consistent with blood ammonia levels. The tailor-made multiple interactions in the confined pore space provide an effective approach for highly sensitive ammonia detection, as well as brings good news to liver and kidney patients for non-invasive diagnosis and real-time health monitoring.