引用本文:
Qin Ming Zhang, Chao Zhan Wang, Jiang Feng Liu, Li Li Wang. A novel protein refolding method integrating ion exchange chromatography with artificial molecular chaperone[J]. Chinese Chemical Letters,
2008, 19(5): 595-598.
doi:
10.1016/j.cclet.2008.03.030
Citation: Qin Ming Zhang, Chao Zhan Wang, Jiang Feng Liu, Li Li Wang. A novel protein refolding method integrating ion exchange chromatography with artificial molecular chaperone[J]. Chinese Chemical Letters, 2008, 19(5): 595-598. doi: 10.1016/j.cclet.2008.03.030
Citation: Qin Ming Zhang, Chao Zhan Wang, Jiang Feng Liu, Li Li Wang. A novel protein refolding method integrating ion exchange chromatography with artificial molecular chaperone[J]. Chinese Chemical Letters, 2008, 19(5): 595-598. doi: 10.1016/j.cclet.2008.03.030
A novel protein refolding method integrating ion exchange chromatography with artificial molecular chaperone
摘要:
Artificial molecular chaperone (AMC) and ion exchange chromatography (IEC) were integrated, thus a new refolding method,artificial molecular chaperone-ion exchange chromatography (AMC-IEC) was developed. Compared with AMC and IEC, theactivity recovery of lysozyme obtained by AMC-IEC was much higher in the investigated range of initial protein concentrations,and the results show that AMC-IEC is very efficient for protein refolding at high concentrations. When the initial concentration oflysozyme is 180 mg/mL, its activity recovery obtained by AMC-IEC is still as high as 76.6%, while the activity recoveries obtainedby AMC and IEC are 45.6% and 42.4%, respectively.
English
A novel protein refolding method integrating ion exchange chromatography with artificial molecular chaperone
Abstract:
Artificial molecular chaperone (AMC) and ion exchange chromatography (IEC) were integrated, thus a new refolding method,artificial molecular chaperone-ion exchange chromatography (AMC-IEC) was developed. Compared with AMC and IEC, theactivity recovery of lysozyme obtained by AMC-IEC was much higher in the investigated range of initial protein concentrations,and the results show that AMC-IEC is very efficient for protein refolding at high concentrations. When the initial concentration oflysozyme is 180 mg/mL, its activity recovery obtained by AMC-IEC is still as high as 76.6%, while the activity recoveries obtainedby AMC and IEC are 45.6% and 42.4%, respectively.
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