Drug and Plasmid DNA Co-Delivery Nanocarriers Based on ABC-Type Polypeptide Hybrid Miktoarm Star Copolymers

Tao Liu Yan-feng Zhang Shi-yong Liu

Citation:  Tao Liu, Yan-feng Zhang, Shi-yong Liu. Drug and Plasmid DNA Co-Delivery Nanocarriers Based on ABC-Type Polypeptide Hybrid Miktoarm Star Copolymers[J]. Chinese Journal of Polymer Science, 2013, 31(6): 924-937. doi: 10.1007/s10118-013-1281-0 shu

Drug and Plasmid DNA Co-Delivery Nanocarriers Based on ABC-Type Polypeptide Hybrid Miktoarm Star Copolymers

    通讯作者: Shi-yong Liu,
  • 基金项目:

    The work was financially supported by the National Natural Science Foundation of China (Nos. 21274137, 91027026 and 51033005), Fundamental Research Funds for the Central Universities, and Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP, 20123402130010).

摘要: We report on the fabrication of self-assembled micelles from ABC-type miktoarm star polypeptide hybrid copolymers consisting of poly(ethylene oxide), poly(L-lysine), and poly(e-caprolactone) arms, PEO(-b-PLL)-b-PCL, and their functional applications as co-delivery nanocarriers of chemotherapeutic drugs and plasmid DNA. Miktoarm star copolymer precursors, PEO(-b-PZLL)-b-PCL, were synthesized at first via the combination of consecutive click reactions and ring-opening polymerizations (ROP), where PZLL is poly(-benzyloxycarbonyl-L-lysine). Subsequently, the deprotection of PZLL arm afforded amphiphilic miktoarm star copolymers, PEO(-b-PLL)-b-PCL. In aqueous media at pH 7.4, PEO(-b-PLL)-b-PCL self-assembles into micelles consisting of PCL cores and hydrophilic PEO/PLL hybrid coronas. The hydrophobic micellar cores can effectively encapsulate model hydrophobic anticancer drug, paclitaxel; whereas positively charged PLL arms within mixed micellar corona are capable of forming electrostatic polyplexes with negatively charged plasmid DNA (pDNA) at N/P ratios higher than ca. 2. Thus, PEO(-b-PLL)-b-PCL micelles can act as co-delivery nanovehicles for both chemotherapeutic drugs and genes. Furthermore, polyplexes of pDNA with paclitaxel-loaded PEO(-b-PLL)-b-PCL micelles exhibited improved transfection efficiency compared to that of pDNA/blank micelles. We expect that the reported strategy of varying chain topologies for the fabrication of co-delivery polymeric nanocarriers can be further applied to integrate with other advantageous functions such as targeting, imaging, and diagnostics.

English


    1. [1]

      [1] Deming, T.J., Adv. Mater., 1997, 9: 299 [2] Trzcinska, R., Szweda, D[J]., Rangelov, S., Suder, P., Silberring, J., Dworak, A. and Trzebicka, B., J. Polym. Sci., Part A Polym. Chem.2012, 50:3104- [3] Zhu, M.Q., Xiang, L., Yang, K., Shen, L.J., Long, F., Fan, J.B., Yi, H.Q., Xiang, J. and Aldred, M.P., J. Polym. Res., 2012, 19: 9808 [4] Zhang, S.S., Chen, C[J].Y. and Li, Z.B., Chinese J. Polym. Sci.2013, 31(2):201- [5] Li, H.B., Tian, Z., Zhang, A.Y. and Feng, Z.G., Chinese J. Polym. Sci., 2009, 27(3): 317 [6] Gebhardt, K.E., Ahn, S[J]., Venkatachalam, G. and Savin, D.A., J. Colloid Interface Sci.2008, 317:70- [7] Li, C.H., Ge, Z[J].S., Fang, J. and Liu, S.Y., Macromolecules.2009, 42:2916- [8] Xu, X.H., Li, C.X., Li, H.P., Liu, R., Jiang, C., Wu, Y., He, B. and Gu, Z.W., Sci. Chin. Chem., 2011, 54: 326 [9] Cho, C.S., Cheon, J.B., Jeong, Y.I., Kim, I.S., Kim, S.H. and Akaike, T., Macromol. Rapid Commun., 1997, 18: 361 [10] Babin, J., Rodriguez-Hernandez, J., Lecommandoux, S., Klok, H.A. and Achard, M.F., Faraday Discuss., 2005, 128: 179 [11] Dimitrov, I. and Schlaad, H., Chem. Commun., 2003, 2944 [12] Lubbert, A., Castelletto, V., Hamley, I.W., Nuhn, H., Scholl, M., Bourdillon, L., Wandrey, C. and Klok, H.A., Langmuir, 2005, 21: 6582 [13] Checot, F., Rodriguez-Hernandez, J., Gnanou, Y. and Lecommandoux, S., Polym. Adv. Technol., 2006, 17: 782 [14] Schlaad, H., Smarsly, B. and Below, I., Macromolecules, 2006, 39: 4631 [15] Checot, F., Rodriguez-Hernandez, J[J]., Gnanou, Y. and Lecommandoux, S., Biomol. Eng.2007, 24:81- [16] Gil, G.O., Losik, M., Schlaad, H., Drechsler, M. and Hellweg, T., Langmuir, 2008, 24: 12823 [17] Sun, J., Chen, X.S., Deng, C., Yu, H.J., Xie, Z.G. and Jing, X.B., Langmuir, 2007, 23: 8308 [18] Sun, J., Deng, C., Chen, X.S., Yu, H.J., Tian, H.Y., Sun, J.R. and Jing, X.B., Biomacromolecules, 2007, 8: 1013 [19] Kricheldorf, H.R. and Hauser, K[J]., Biomacromolecules.2001, 2:1110- [20] Caillol, S., Lecommandoux, S., Mingotaud, A.F., Schappacher, M., Soum, A., Bryson, N. and Meyrueix, R., Macromolecules, 2003, 36: 1118 [21] Rong, G.Z., Deng, M.X., Deng, C., Tang, Z.H., Piao, L.H., Chen, X.S. and Jing, X.B., Biomacromolecules, 2003, 4: 1800 [22] Sun, J., Chen, X.S., Lu, T.C., Liu, S., Tian, H.Y., Guo, Z.P. and Jing, X.B., Langmuir, 2008, 24: 10099 [23] Holowka, E.P., Pochan, D[J].J. and Deming, T.J., J. Am. Chem. Soc.2005, 127:12423- [24] Rodriguez-Hernandez, J. and Lecommandoux, S[J]., J. Am. Chem. Soc.2005, 127:2026- [25] Rao, J.Y., Luo, Z.F., Ge, Z.S., Liu, H. and Liu, S.Y., Biomacromolecules, 2007, 8: 3871 [26] Zhang, X.Q., Li, J.G., Li, W. and Zhang, A., Biomacromolecules, 2007, 8: 3557 [27] Pitsikalis, M., Pispas, S[J]., Mays, J.W. and Hadjichristidis, N., Adv. Polym. Sci.1998, 135:1- [28] Babin, J., Leroy, C., Lecommandoux, S., Borsali, R., Gnanou, Y. and Taton, D., Chem. Commun., 2005, 1993 [29] Abraham, S., Ha, C[J].S. and Kim, I., J. Polym. Sci., Part A Polym. Chem.2006, 44:2774- [30] Babin, J., Taton, D., Brinkmann, M. and Lecommandoux, S., Macromolecules, 2008, 41: 1384 [31] Rao, J.Y., Zhang, Y.F., Zhang, J.Y. and Liu, S.Y., Biomacromolecules, 2008, 9: 2586 [32] Li, Z.B., Kesselman, E., Talmon, Y., Hillmyer, M.A. and Lodge, T.P., Science, 2004, 306: 98 [33] Li, Z.B., Hillmyer, M[J].A. and Lodge, T.P., Nano Lett.2006, 6:1245- [34] Li, Z.B., Hillmyer, M.A. and Lodge, T.P., Langmuir, 2006, 22: 9409 [35] Zhang, Y.F., Liu, H[J]., Dong, H.F., Li, C.H. and Liu, S.Y., J. Polym. Sci., Part A Polym. Chem.2009, 47:1636- [36] Zhang, W.D., Zhang, W., Zhang, Z.B., Cheng, Z.P., Tu, Y.F., Qiu, Y.S. and Zhu, X.L., J. Polym. Sci., Part A Polym. Chem., 2010, 48: 4268 [37] Du, J., Sun, Y., Shi, Q.S., Liu, P.F., Zhu, M.J., Wang, C.H., Du, L.F. and Duan, Y.R., Int. J. Mol. Sci., 2012, 13: 516 [38] Zhou, D.Z., Li, C.X., Hu, Y.L., Zhou, H., Chen, J.T., Zhang, Z.P. and Guo, T.Y., J. Mater. Chem., 2012, 22: 10743 [39] Mintzer, M.A. and Simanek, E[J].E., Chem. Rev.2009, 109:259- [40] Meng, F.H., Hennink, W.E. and Zhong, Z., Biomaterials, 2009, 30: 2180 [41] Janat-Amsbury, M.M., Yockman, J.W., Lee, M., Kern, S., Furgeson, D.Y., Bikram, M. and Kim, S.W., Mol. Ther., 2004, 9: 829 [42] Yue, X.Y., Qiao, Y., Qiao, N., Guo, S.T., Xing, J.F., Deng, L.D., Xu, J.Q. and Dong, A.J., Biomacromolecules, 2010, 11: 2306 [43] Lu, X., Wang, Q.Q., Xu, F.J., Tang, G.P. and Yang, W.T., Biomaterials, 2011, 32: 4849 [44] Sun, T.M., Du, J.Z., Yao, Y.D., Mao, C.Q., Dou, S., Huang, S.Y., Zhang, P.Z., Leong, K.W., Song, E.W. and Wang, J., Acs Nano, 2011, 5: 1483 [45] Xiong, X.B. and Lavasanifar, A[J]., Acs Nano.2011, 5:5202- [46] Wang, Y., Gao, S.J., Ye, W.H., Yoon, H.S. and Yang, Y.Y., Nat. Mater., 2006, 5: 791 [47] Qiu, L.Y. and Bae, Y[J].H., Biomaterials.2007, 28:4132- [48] Guo, S.T., Qiao, Y., Wang, W.W., He, H.Y., Deng, L.D., Xing, J.F., Xu, J.Q., Liang, X.J. and Dong, A.J., J. Mater. Chem., 2010, 20: 6935 [49] Bromberg, L. and Levin, G[J]., Bioconjugate Chem.1998, 9:40- [50] Wu, P., Feldman, A.K., Nugent, A.K., Hawker, C.J., Scheel, A., Voit, B., Pyun, J., Frechet, J.M.J., Sharpless, K.B. and Fokin, V.V., Angew. Chem., Int. Ed., 2004, 43: 3928 [51] Lewis, W.G., Magallon, F.G., Fokin, V.V. and Finn, M.G., J. Am. Chem. Soc., 2004, 126: 9152 [52] Altintas, O., Hizal, G[J].and Tunca, U., J. Polym. Sci., Part A Polym. Chem.2006, 44:5699- [53] Kolb, H.C., Finn, M[J].G. and Sharpless, K.B., Angew. Chem., Int. Ed.2001, 40:2004- [54] Qin, A.J., Lam, J.W.Y. and Tang, B.Z., Macromolecules, 2010, 43: 8693 [55] Fu, R. and Fu, G[J].D., Polym. Chem.2011, 2:465- [56] Agut, W., Taton, D[J].and Lecommandoux, S., Macromolecules.2007, 40:5653- [57] Agut, W., Agnaou, R[J]., Lecommandoux, S. and Taton, D., Macromol. Rapid Commun.2008, 29:1147- [58] Wang, K., Liang, L.Y., Lin, S.L. and He, X.H., Eur. Polym. J., 2008, 44: 3370 [59] Liu, T., Qian, Y.F., Hu, X.L., Ge, Z.S. and Liu, S.Y., J. Mater. Chem., 2012, 22: 5020 [60] Li, X.J., Qian, Y.F., Liu, T., Hu, X.L., Zhang, G.Y., You, Y.Z. and Liu, S.Y., Biomaterials, 2011, 32: 6595 [61] Zhu, C.H., Jung, S., Luo, S.B., Meng, F.H., Zhu, X.L., Park, T.G. and Zhong, Z.Y., Biomaterials, 2010, 31: 2408 [62] Yue, Y.A., Jin, F., Deng, R., Cai, J.G., Chen, Y.C., Lin, M.C.M., Kung, H.F. and Wu, C., J. Controlled Release, 2011, 155: 67

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  • 发布日期:  2013-06-05
  • 收稿日期:  2013-01-07
  • 修回日期:  2013-02-05
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