In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane

Nong Yue HE Jian Xin TANG Song LI Hong CHEN An Cun ZHOU

引用本文: Nong Yue HE,  Jian Xin TANG,  Song LI,  Hong CHEN,  An Cun ZHOU. In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane[J]. Chinese Chemical Letters, 2005, 16(3): 397-400. shu
Citation:  Nong Yue HE,  Jian Xin TANG,  Song LI,  Hong CHEN,  An Cun ZHOU. In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane[J]. Chinese Chemical Letters, 2005, 16(3): 397-400. shu

In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane

  • 基金项目:

    This research is supported by the Trans-Century Training Programme Foundation for the Talents by the Ministry of Education of China and the Chinese Postdoctoral Science Foundation (2003).

摘要: Polypropylene microporous membranes were treated with plasma in a mixture of N2 and H2 (1:2 in volume). Attenuated total reflectance Fourier transform infrared spectroscopy(ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and ultra-violet (UV) spectra demonstratedthe success of grafting amino groups. The density of the polar amino groups on the membrane surface is about 0.59 μmol/cm2. The as-treated membranes were successively applied to the in situ synthesis of oligonucleotides and an average coupling yield was more than 98%. The surface feature of the treated membrane is suggested to be responsible for its advantage over a glass slide.

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  • 收稿日期:  2004-02-27
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