引用本文:
Song LI, Jian Xin TANG, Mei Ju JI, Peng HOU, Peng Feng XIAO, Nong Yue HE. A Novel Substrate for in situ Synthesis of Oligonucleotides:Hydrolyzed Microporous Polyamide-6 Membrane[J]. Chinese Chemical Letters,
2004, 15(7): 837-840.
Citation: Song LI, Jian Xin TANG, Mei Ju JI, Peng HOU, Peng Feng XIAO, Nong Yue HE. A Novel Substrate for in situ Synthesis of Oligonucleotides:Hydrolyzed Microporous Polyamide-6 Membrane[J]. Chinese Chemical Letters, 2004, 15(7): 837-840.
Citation: Song LI, Jian Xin TANG, Mei Ju JI, Peng HOU, Peng Feng XIAO, Nong Yue HE. A Novel Substrate for in situ Synthesis of Oligonucleotides:Hydrolyzed Microporous Polyamide-6 Membrane[J]. Chinese Chemical Letters, 2004, 15(7): 837-840.
A Novel Substrate for in situ Synthesis of Oligonucleotides:Hydrolyzed Microporous Polyamide-6 Membrane
摘要:
A novel substrate for in situ synthesis of oligonucleotide was prepared by hydrolyzing microporous polyamide-6 membranes in a 0.01mol/L NaOH/(H2O-CH3OH) mixture medium. The formation of amines (NH2) on the surface was proved by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The treated membrane was applied for in situ synthesis of oligonucleotide and a single step coupling efficiency determined by ultraviolet (UV) spectra was above 98%.
English
A Novel Substrate for in situ Synthesis of Oligonucleotides:Hydrolyzed Microporous Polyamide-6 Membrane
Abstract:
A novel substrate for in situ synthesis of oligonucleotide was prepared by hydrolyzing microporous polyamide-6 membranes in a 0.01mol/L NaOH/(H2O-CH3OH) mixture medium. The formation of amines (NH2) on the surface was proved by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The treated membrane was applied for in situ synthesis of oligonucleotide and a single step coupling efficiency determined by ultraviolet (UV) spectra was above 98%.
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