引用本文:
Jian Wei LI, Fang TIAN, Chen WANG, Chun Li BAI, En Hua CAO. A Possible Multi-stranded DNA Induced by Acid Denaturation-renaturation[J]. Chinese Chemical Letters,
1997, 8(1): 49-52.
Citation: Jian Wei LI, Fang TIAN, Chen WANG, Chun Li BAI, En Hua CAO. A Possible Multi-stranded DNA Induced by Acid Denaturation-renaturation[J]. Chinese Chemical Letters, 1997, 8(1): 49-52.
Citation: Jian Wei LI, Fang TIAN, Chen WANG, Chun Li BAI, En Hua CAO. A Possible Multi-stranded DNA Induced by Acid Denaturation-renaturation[J]. Chinese Chemical Letters, 1997, 8(1): 49-52.
A Possible Multi-stranded DNA Induced by Acid Denaturation-renaturation
摘要:
λ-DNA Hind Ⅲ was denatured by HCl,renatured by NaOH,and digested by DNase I sequentially.The produced mixture was separated with Sephadex G200 column,and the chromatograph showed a very weak peak of one component resistant to the digestion of DNase I.The results of AFM studies on this component indicated that an unusual form of DNA was present,with the height 13 times that of double-stranded DNA.By comparison with known results,it is suggested that this is a multi-stranded DNA other than simple supercoils.
English
A Possible Multi-stranded DNA Induced by Acid Denaturation-renaturation
Abstract:
λ-DNA Hind Ⅲ was denatured by HCl,renatured by NaOH,and digested by DNase I sequentially.The produced mixture was separated with Sephadex G200 column,and the chromatograph showed a very weak peak of one component resistant to the digestion of DNase I.The results of AFM studies on this component indicated that an unusual form of DNA was present,with the height 13 times that of double-stranded DNA.By comparison with known results,it is suggested that this is a multi-stranded DNA other than simple supercoils.
扫一扫看文章
计量
- PDF下载量: 0
- 文章访问数: 0
- HTML全文浏览量: 0
下载:
