引用本文:
Jing He YANG, Chang Lun TONG, Nian Qin JIE, Hong Yu GONG, Zu Quan GAO, Hua Bin ZOU. STUDY OF THE FLUORESCENCE SYSTEM OF RIBONUCLEIC ACID-Tb（Ⅲ） AND THE DETERMINATION OF RIBONUCLEIC ACID[J]. Chinese Chemical Letters,
1995, 6(2): 135-138.
Citation: Jing He YANG, Chang Lun TONG, Nian Qin JIE, Hong Yu GONG, Zu Quan GAO, Hua Bin ZOU. STUDY OF THE FLUORESCENCE SYSTEM OF RIBONUCLEIC ACID-Tb（Ⅲ） AND THE DETERMINATION OF RIBONUCLEIC ACID[J]. Chinese Chemical Letters, 1995, 6(2): 135-138.
Citation: Jing He YANG, Chang Lun TONG, Nian Qin JIE, Hong Yu GONG, Zu Quan GAO, Hua Bin ZOU. STUDY OF THE FLUORESCENCE SYSTEM OF RIBONUCLEIC ACID-Tb（Ⅲ） AND THE DETERMINATION OF RIBONUCLEIC ACID[J]. Chinese Chemical Letters, 1995, 6(2): 135-138.
STUDY OF THE FLUORESCENCE SYSTEM OF RIBONUCLEIC ACID-Tb（Ⅲ） AND THE DETERMINATION OF RIBONUCLEIC ACID
摘要:
It was found that ribonucleic acid(RNA)compiexes with Tb(Ⅲ)at pH=5.0-6.5,which then emits strong characterlstic fluorescence of Tb(Ⅲ).The excitation and emission wavelengths are 288nm,494nm and 545nm,respectively.A linear relationship is obtained between the fluorescence intensity and RNA concentration in the range of 0.1 ug/ml-10 ug/ml.The detection limit is 8.0×10-10mol/L.This method can be used to determine RNA in the presence of adenylic acid,uridylic acid and cytidylic aci.
English
STUDY OF THE FLUORESCENCE SYSTEM OF RIBONUCLEIC ACID-Tb（Ⅲ） AND THE DETERMINATION OF RIBONUCLEIC ACID
Abstract:
It was found that ribonucleic acid(RNA)compiexes with Tb(Ⅲ)at pH=5.0-6.5,which then emits strong characterlstic fluorescence of Tb(Ⅲ).The excitation and emission wavelengths are 288nm,494nm and 545nm,respectively.A linear relationship is obtained between the fluorescence intensity and RNA concentration in the range of 0.1 ug/ml-10 ug/ml.The detection limit is 8.0×10-10mol/L.This method can be used to determine RNA in the presence of adenylic acid,uridylic acid and cytidylic aci.
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