超临界流体色谱同时测定运动饮料中的8种邻苯二甲酸酯类增塑剂

引用本文: 韦俊芳, 姜磊, 楼超艳, 朱岩. 超临界流体色谱同时测定运动饮料中的8种邻苯二甲酸酯类增塑剂[J]. 色谱, 2018, 36(7): 678-684. doi: 10.3724/SP.J.1123.2018.01014 shu

Citation: WEI Junfang, JIANG Lei, LOU Chaoyan, ZHU Yan. Simultaneous determination of eight phthalate ester plasticizers in sports beverages using supercritical fluid chromatography[J]. Chinese Journal of Chromatography, 2018, 36(7): 678-684. doi: 10.3724/SP.J.1123.2018.01014 shu

超临界流体色谱同时测定运动饮料中的8种邻苯二甲酸酯类增塑剂

韦俊芳 ^1, ,
姜磊 ^2, ,
楼超艳 ^3, ,
朱岩 ^3,

1.
浙江大学公共体育与艺术部, 浙江杭州 310000;
2.
浙江省纺织测试研究院, 浙江杭州 310013;
3.
浙江大学化学系, 浙江杭州 310000

摘要: 建立了同时快速测定运动饮料中邻苯二甲酸二甲酯、邻苯二甲酸二乙酯、邻苯二甲酸二丙酯、邻苯二甲酸二丁酯、邻苯二甲酸二戊酯、邻苯二甲酸丁基苄基酯、邻苯二甲酸二（2-乙基己基）酯、邻苯二甲酸二辛酯8种邻苯二甲酸酯（PAEs）类增塑剂的超临界流体色谱-紫外检测分析方法。实际样品经液相萃取后，用超临界流体色谱分析，以超临界态二氧化碳-3%（体积分数）甲醇作为色谱流动相进行等度洗脱，检测波长为225 nm，6 min即可实现分离。8种PAE类增塑剂在0.05~25 mg/L范围内均具有良好的线性关系，相关系数为0.9991~0.9997，方法检出限为7.5~15 μg/L。8种PAE类增塑剂在运动饮料样品中的加标回收率为91.7%~100.2%，相对标准偏差均不大于6.5%（n=3）。应用该方法对多种市售运动饮料中的8种PAE类增塑剂进行检测，结果表明，该方法环保、快速、灵敏、选择性高、结果准确，能满足运动饮料中增塑剂含量检测的要求。

关键词:

English

Simultaneous determination of eight phthalate ester plasticizers in sports beverages using supercritical fluid chromatography

1.
Department of Public Physical Art Education, Zhejiang University, Hangzhou 310000, China;
2.
Zhejiang Textile Testing Research Institute, Hangzhou 310013, China;
3.
Department of Chemistry, Zhejiang University, Hangzhou 310000, China
Received Date: 09 January 2018

Abstract: Phthalate esters (PAEs) are a group of serious environmental pollutants that are carcinogenic or tumorigenic to humans. In this work, a green, rapid, and effective analytical method based on supercritical fluid chromatography (SFC) has been proposed for the simultaneous determination of the eight PAE plasticizers in sports beverage samples. They are dimethyl phthalate (DMP), diethyl phthalate (DEP), dipropyl phthalate (DPRP), dibutyl phthalate (DBP), dipentyl phthalate (DPP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), and di-n-octyl phthalate (DNOP). Liquid phase extraction (LPE) was employed to extract the PAE plasticizers before testing using SFC with ultraviolet (UV) detection. SFC parameters, including stationary phase screening, modifier composition and volume percentage, column temperature, flow rate, and backpressure, were optimized. Under the optimum conditions, all the eight PAE plasticizers could be determined simultaneously by SFC within 6 min at 225 nm. The optimized eluent was supercritical CO₂ with 3% (v/v) methanol. The performance of the developed method was also evaluated. The eight PAE plasticizers exhibited satisfactory linearities with correlation coefficients (r) of 0.9991-0.9997 in the range of 0.05-25 mg/L. The limits of detection ranged from 7.5 to 15 μg/L (S/N=3, n=3). Recoveries of all the PAE plasticizers for the spiked samples ranged from 91.7%-100.2% with relative standard deviations of no more than 6.5% (n=3). This method is green, time-saving, simple, selective, robust, and convenient for the analysis of the eight PAE plasticizers in real sports beverage samples.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

超临界流体色谱同时测定运动饮料中的8种邻苯二甲酸酯类增塑剂

English

Simultaneous determination of eight phthalate ester plasticizers in sports beverages using supercritical fluid chromatography

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中国化学会秘书处

超临界流体色谱同时测定运动饮料中的8种邻苯二甲酸酯类增塑剂

English

Simultaneous determination of eight phthalate ester plasticizers in sports beverages using supercritical fluid chromatography

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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