Hydrolysis of Dipeptide Heptyl Esters with Newlase F

引用本文: Zhong Zhou CHEN, Yan Mei LI, Xiu Fen KOU, Xue LI, Fu Rong HUANG, Hai Yan WANG, Ming Ming MA, Yu Fen ZHAO. Hydrolysis of Dipeptide Heptyl Esters with Newlase F[J]. Chinese Chemical Letters, 2001, 12(8): 693-696. shu

Citation: Zhong Zhou CHEN, Yan Mei LI, Xiu Fen KOU, Xue LI, Fu Rong HUANG, Hai Yan WANG, Ming Ming MA, Yu Fen ZHAO. Hydrolysis of Dipeptide Heptyl Esters with Newlase F[J]. Chinese Chemical Letters, 2001, 12(8): 693-696. shu

Hydrolysis of Dipeptide Heptyl Esters with Newlase F

1.
Key Laboratory for Bioorganic Phosphorus Chemistry of Education Ministry, Department of Chemistry, School of Life Science and Engineering, Tsinghua University, Beijing 100084;
2.
The Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080
基金项目:
Newlase F was provided by Amano pharmaceutical Co. Ltd. freely. The authors would like to thank the financial supports from the National Natural Science Foundation of China (No. 29802006) and the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of MOE, P.R.C.

摘要: Newlase F is a rude enzyme which contains triacylglycerol lipase and acid protease. Hydrolysis of dipeptide heptyl esters with Newlase F was studied in phosphate buffer-organic solvent by HPLC. When the Newlase F was level reached 5 mg/mL under mild condition (pH 7.0, 30℃), the lipase had the highest activity. The reaction was also affected greatly by organic solvents and their concentrations. It is found that protease in Newlase F does not hydrolyze amide bond under this condition (pH 7.0, r.t.).

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English

Hydrolysis of Dipeptide Heptyl Esters with Newlase F

1.
Key Laboratory for Bioorganic Phosphorus Chemistry of Education Ministry, Department of Chemistry, School of Life Science and Engineering, Tsinghua University, Beijing 100084;
2.
The Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080
Received Date: 15 January 2001
Fund Project:
Newlase F was provided by Amano pharmaceutical Co. Ltd. freely. The authors would like to thank the financial supports from the National Natural Science Foundation of China (No. 29802006) and the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of MOE, P.R.C.

Abstract: Newlase F is a rude enzyme which contains triacylglycerol lipase and acid protease. Hydrolysis of dipeptide heptyl esters with Newlase F was studied in phosphate buffer-organic solvent by HPLC. When the Newlase F was level reached 5 mg/mL under mild condition (pH 7.0, 30℃), the lipase had the highest activity. The reaction was also affected greatly by organic solvents and their concentrations. It is found that protease in Newlase F does not hydrolyze amide bond under this condition (pH 7.0, r.t.).

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Hydrolysis of Dipeptide Heptyl Esters with Newlase F

English

Hydrolysis of Dipeptide Heptyl Esters with Newlase F

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Hydrolysis of Dipeptide Heptyl Esters with Newlase F

English

Hydrolysis of Dipeptide Heptyl Esters with Newlase F

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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