Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging

引用本文: Ting Wu, Xiao-Hui Yang, Chuan-Jing Zhang, Zhen-Ping Wang, Yi-Ping Du. Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging[J]. Chinese Chemical Letters, 2016, 27(6): 901-904. doi: 10.1016/j.cclet.2016.02.020 shu

Citation: Ting Wu, Xiao-Hui Yang, Chuan-Jing Zhang, Zhen-Ping Wang, Yi-Ping Du. Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging[J]. Chinese Chemical Letters, 2016, 27(6): 901-904. doi: 10.1016/j.cclet.2016.02.020 shu

Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging

Shanghai Key Laboratory of Functional Materials Chemistry, Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai 200237, China
基金项目:
The authors acknowledge Dr. Yaxin Peng for the assistance on data interpretation. This study was financially supported by the National Natural Science Foundation of China (No. 21205041), the Fundamental Research Funds for the Central Universities (No. 222201314039), and a grant from Shanghai Municipal Education Committee (No. YJ0114209).

摘要: Quantification of a mixture of peptides in solution was achieved by disposable patterned hydrophilic chip based matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI MSI). Compared with other quantitative methods for peptides in solution, this method is label-free and does not require separation of the multiple components of the solution before analysis. Uniform hydrophilic spots and high mass accuracy measurements provided confident identification and quantitative analysis of imaged compounds. The linear correlation between concentration and grayscale of image in the range of 5 fmol/mL to 1 pmol/mL was obtained for all four peptides. Good sensitivity and excellent reproducibility were also achieved. The method expands the application of MALDI MSI from tissues to solutions.

关键词:

English

Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging

Shanghai Key Laboratory of Functional Materials Chemistry, Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai 200237, China
Received Date: 14 December 2015
Revised Date: 22 January 2016
Fund Project:
The authors acknowledge Dr. Yaxin Peng for the assistance on data interpretation. This study was financially supported by the National Natural Science Foundation of China (No. 21205041), the Fundamental Research Funds for the Central Universities (No. 222201314039), and a grant from Shanghai Municipal Education Committee (No. YJ0114209).

Abstract: Quantification of a mixture of peptides in solution was achieved by disposable patterned hydrophilic chip based matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI MSI). Compared with other quantitative methods for peptides in solution, this method is label-free and does not require separation of the multiple components of the solution before analysis. Uniform hydrophilic spots and high mass accuracy measurements provided confident identification and quantitative analysis of imaged compounds. The linear correlation between concentration and grayscale of image in the range of 5 fmol/mL to 1 pmol/mL was obtained for all four peptides. Good sensitivity and excellent reproducibility were also achieved. The method expands the application of MALDI MSI from tissues to solutions.

Key words:

1. [1] B. Cillero-Pastor, R.M.A. Heeren, Matrix-assisted laser desorption ionization mass spectrometry imaging for peptide and protein analyses: a critical review of ontissue digestion, J. Proteome Res. 13 (2014) 325-335.
2. [2] R.M. Caprioli, T.B. Farmer, J. Gile, Molecular imaging of biological samples: localization of peptides and proteins using MALDI-TOF MS, Anal. Chem. 69 (1997) 4751-4760.
3. [3] D.S. Cornett, M.L. Reyzer, P. Chaurand, R.M. Caprioli, MALDI imaging mass spectrometry: molecular snapshots of biochemical systems, Nat. Methods 4 (2007) 828-833.
4. [4] I.D. Grishin, MALDI TOF mass spectrometry for the investigation of the structure of ruthenium carborane complexes, J. Anal. Chem. 68 (2013) 1123-1129.
5. [5] R.Q. Wang, K. Druckenmüller, G. Elbers, K. Guenther, J.-P. Croué, Analysis of aquatic-phase natural organic matter by optimized LDI-MS method, J. Mass Spectrom. 49 (2014) 154-160.
6. [6] P.J. Todd, T.G. Schaaff, P. Chaurand, R.M. Caprioli, Organic ion imaging of biological tissue with secondary ion mass spectrometry and matrix-assisted laser desorption/ionization, J. Mass Spectrom. 36 (2001) 355-369.
7. [7] F. Tang, Y. Chen, T.G. Li, et al., A quick and effective multivariate statistical strategy for imaging mass spectrometry, Chin. Chem. Lett. 25 (2014) 1331-1335.
8. [8] M.L. Reyzer, Y.S. Hsieh, K. Ng, W.A. Korfmacher, R.M. Caprioli, Direct analysis of drug candidates in tissue by matrix-assisted laser desorption/ionization mass spectrometry, J. Mass Spectrom. 38 (2003) 1081-1092.
9. [9] L.A. McDonnell, R.M.A. Heeren, Imaging mass spectrometry, Mass Spectrom. Rev. 26 (2007) 606-643.
10. [10] M.R. Groseclose, M. Andersson, W.M. Hardesty, R.M. Caprioli, Identification of proteins directly from tissue: in situ tryptic digestions coupled with imaging mass spectrometry, J. Mass Spectrom. 42 (2007) 254-262.
11. [11] M. Stoeckli, D. Staab, M. Staufenbiel, K.-H. Wiederhold, L. Signor, Molecular imaging of amyloid b peptides in mouse brain sections using mass spectrometry, Anal. Biochem. 311 (2002) 33-39.
12. [12] K. Krueger, C. Terne, C. Werner, et al., Characterization of polymer membranes by MALDI mass-spectrometric imaging techniques, Anal. Chem. 85 (2013) 4998-5004.
13. [13] R. Ait-Belkacem, C. Berenguer, C. Villard, et al., Monitoring therapeutic monoclonal antibodies in brain tumor, MAbs 6 (2014) 1385-1393.
14. [14] R. Longuespée, C. Boyon, A. Desmons, et al., Spectroimmunohistochemistry: a novel form of MALDI mass spectrometry imaging coupled to immunohistochemistry for tracking antibodies, OMICS-A J. Integr. Biol. 18 (2014) 132-141.
15. [15] V. Mainini, G. Bovo, C. Chinello, et al., Detection of high molecular weight proteins by MALDI imaging mass spectrometry, Mol. Biosyst. 9 (2013) 1101-1107.
16. [16] K. Kristjansdottir, S.J. Kron, Stable-isotope labeling for protein quantitation by mass spectrometry, Curr. Proteomics 7 (2010) 144-155.
17. [17] W.W. Wu, G.H. Wang, S.J. Baek, R.F. Shen, Comparative study of three proteomic quantitative methods, DIGE, cICAT, and iTRAQ, using 2D gel-or LC-ALDI TOF/TOF, J. Proteome Res. 5 (2006) 651-658.
18. [18] P.J. Boersema, R. Raijmakers, S. Lemeer, S. Mohammed, A.J.R. Heck, Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics, Nat. Protoc. 4 (2009) 484-494.
19. [19] M. Bantscheff, M. Schirle, G. Sweetman, J. Rick, B. Kuster, Quantitative mass spectrometry in proteomics: a critical review, Anal. Bioanal. Chem. 389 (2007) 1017-1031.
20. [20] Y.D. Kim, C.B. Park, D.S. Clark, Stable sol-gel microstructured and microfluidic networks for protein patterning, Biotechnol. Bioeng. 73 (2001) 331-337.
21. [21] M. Lee, K.H. Kim, J.G. Park, et al., Fabrication of a hydrophobic/hydrophilic hybridpatterned microarray chip and its application to a cancer marker immunoassay, BioChip J. 6 (2012) 10-16.
22. [22] L.A. McDonnell, A. Römpp, B. Balluff, et al., Discussion point: reporting guidelines for mass spectrometry imaging, Anal. Bioanal. Chem. 407 (2015) 2035-2045.
23. [23] S.H. Zuo, Y.J. Teng, H.H. Yuan, M.B. Lan, Direct electrochemistry of glucose oxidase on screen-printed electrodes through one-step enzyme immobilization process with silica sol-gel/polyvinyl alcohol hybrid film, Sens. Actuators, B: Chem. 133 (2008) 555-560.
24. [24] N. Takai, Y. Tanaka, K. Inazawa, H. Saji, Quantitative analysis of pharmaceutical drug distribution in multiple organs by imaging mass spectrometry, Rapid Commun. Mass Spectrom. 26 (2012) 1549-1556.

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Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging

English

Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging

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Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging

English

Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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