引用本文:
ZHi Qiang WANG, Gong ZHANG, Qi Min LIU, Jia Zan NI. FLUORESCENCE SPECTROSCOPY STUDIES ON TIIE ACTION OK RARE EARTHS WITH BOVINE SERUM ALBUMIN IN MULTIMOLECULAR SYSTEM[J]. Chinese Chemical Letters,
1995, 6(10): 879-882.
Citation: ZHi Qiang WANG, Gong ZHANG, Qi Min LIU, Jia Zan NI. FLUORESCENCE SPECTROSCOPY STUDIES ON TIIE ACTION OK RARE EARTHS WITH BOVINE SERUM ALBUMIN IN MULTIMOLECULAR SYSTEM[J]. Chinese Chemical Letters, 1995, 6(10): 879-882.
Citation: ZHi Qiang WANG, Gong ZHANG, Qi Min LIU, Jia Zan NI. FLUORESCENCE SPECTROSCOPY STUDIES ON TIIE ACTION OK RARE EARTHS WITH BOVINE SERUM ALBUMIN IN MULTIMOLECULAR SYSTEM[J]. Chinese Chemical Letters, 1995, 6(10): 879-882.
FLUORESCENCE SPECTROSCOPY STUDIES ON TIIE ACTION OK RARE EARTHS WITH BOVINE SERUM ALBUMIN IN MULTIMOLECULAR SYSTEM
摘要:
In this paper,the interaction of tripositive terbium ions (Tb3+) with bovine serum albumin (DSA) has been investigated in the presence of some alkaline earth metals and citric acid (Cit) by using fluorescence spectroscopy.The results show that Tb3+,BSA and Cit can form ternary complex BSA·Tb2·Cit4 in μ=0.1mol/1 NaCl,pH 6.3 hexaniethylenetetramine buffer.Other rare earths are able to compete for the same binding site in BSA with Tb3+.The sequence of competition is Eu3+ > Pr3+ > Yb3+ > Gd3+ > La3+.Ca2+ and Mg2+ can not replace Tb bound to BSA.
English
FLUORESCENCE SPECTROSCOPY STUDIES ON TIIE ACTION OK RARE EARTHS WITH BOVINE SERUM ALBUMIN IN MULTIMOLECULAR SYSTEM
Abstract:
In this paper,the interaction of tripositive terbium ions (Tb3+) with bovine serum albumin (DSA) has been investigated in the presence of some alkaline earth metals and citric acid (Cit) by using fluorescence spectroscopy.The results show that Tb3+,BSA and Cit can form ternary complex BSA·Tb2·Cit4 in μ=0.1mol/1 NaCl,pH 6.3 hexaniethylenetetramine buffer.Other rare earths are able to compete for the same binding site in BSA with Tb3+.The sequence of competition is Eu3+ > Pr3+ > Yb3+ > Gd3+ > La3+.Ca2+ and Mg2+ can not replace Tb bound to BSA.
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