引用本文:
Hai Yan Wang, Chun Gui Zhao, Ye Hong Zhou, Shao Min Shuang, Chuan Dong. Interference study on the free calcium in the human peripheral lymphocyte by acrylamide[J]. Chinese Chemical Letters,
2008, 19(6): 756-757.
doi:
10.1016/j.cclet.2007.12.023
Citation: Hai Yan Wang, Chun Gui Zhao, Ye Hong Zhou, Shao Min Shuang, Chuan Dong. Interference study on the free calcium in the human peripheral lymphocyte by acrylamide[J]. Chinese Chemical Letters, 2008, 19(6): 756-757. doi: 10.1016/j.cclet.2007.12.023
Citation: Hai Yan Wang, Chun Gui Zhao, Ye Hong Zhou, Shao Min Shuang, Chuan Dong. Interference study on the free calcium in the human peripheral lymphocyte by acrylamide[J]. Chinese Chemical Letters, 2008, 19(6): 756-757. doi: 10.1016/j.cclet.2007.12.023
Interference study on the free calcium in the human peripheral lymphocyte by acrylamide
摘要:
The role of acrylamide on the human peripheral lymphocytes was studied by laser scanning confocal microscopy technique and fluo-3. The calibration value of the apparent dissociation constant (Kd) of the fluo-3-Ca2+ complex was obtained as 4.83×10-7mol/L. Acrylamide ( < 54μg/mL) evoked a rise in free intracellular calcium concentration [Ca2+]i, in a dose dependent manner. Acrylamide induced the increase of [Ca2+]i was discussed in detail.
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关键词:
- Laser scanning confocal microscopy
- / Acrylamide
- / Lymphocytes
- / Fluo-3
- / [Ca2+]i
English
Interference study on the free calcium in the human peripheral lymphocyte by acrylamide
Abstract:
The role of acrylamide on the human peripheral lymphocytes was studied by laser scanning confocal microscopy technique and fluo-3. The calibration value of the apparent dissociation constant (Kd) of the fluo-3-Ca2+ complex was obtained as 4.83×10-7mol/L. Acrylamide ( < 54μg/mL) evoked a rise in free intracellular calcium concentration [Ca2+]i, in a dose dependent manner. Acrylamide induced the increase of [Ca2+]i was discussed in detail.
-
Key words:
- Laser scanning confocal microscopy
- / Acrylamide
- / Lymphocytes
- / Fluo-3
- / [Ca2+]i
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