利用芯片式高场非对称波形离子迁移谱技术快速检测苯丙氨酸

引用本文: 郭大鹏, 汪永欢, 徐天白, 张媛, 李灵锋, 陈金凯, 汪小知, 李鹏, 骆季奎. 利用芯片式高场非对称波形离子迁移谱技术快速检测苯丙氨酸[J]. 分析化学, 2016, 44(4): 617-624. doi: 10.11895/j.issn.0253-3820.150708 shu

Citation: GUO Da-Peng, WANG Yong-Huan, XU Tian-Bai, ZHANG Yuan, LI Ling-Feng, CHEN Jin-Kai, WANG Xiao-Zhi, LI Peng, LUO Ji-Kui. Rapid Determination of Phenylalanine by Micro-chip based Field Asymmetric Waveform Ion Mobility Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2016, 44(4): 617-624. doi: 10.11895/j.issn.0253-3820.150708 shu

利用芯片式高场非对称波形离子迁移谱技术快速检测苯丙氨酸

郭大鹏 ^1, ,
汪永欢 ^1, ,
徐天白 ^1, ,
张媛 ^2, ,
李灵锋 ^1, ,
陈金凯 ^1, ,
汪小知 ^{1,
,} ,
李鹏 ^2, ,
骆季奎 ^1,

1.
¹ 浙江大学信息与电子工程学系, 杭州 310027;
2.
² 苏州微木智能系统有限公司, 苏州 215163

通讯作者: 汪小知

基金项目:
本文系国家自然科学基金青年基金(No.61301046) (No.61301046)

浙江省公益性技术应用研究计划项目(No.2013C31023) (No.2013C31023)

摘要: 采用金属扩散管-芯片式高场非对称波形离子迁移谱(FAIMS)技术对苯丙氨酸进行了快速检测,设定测试压强为250 kPa,金属扩散管温度为190℃,在优化的最佳分析条件下,即:载气流速为2000 mL/min,分离电压为152.8 V时,在正模式下获得了苯丙氨酸的离子特征谱图和补偿电压特征值-0.62 V。另外,利用FAIMS对不同浓度的苯丙氨酸样品气进行了检测,确定了FAIMS检测的定量线性范围为6~20 mg/L和检出限为5.9 mg/L。本实验为FAIMS应用于苯丙氨酸的快速检测提供了重要参考。

关键词:

English

Rapid Determination of Phenylalanine by Micro-chip based Field Asymmetric Waveform Ion Mobility Spectrometry

1.
¹ Department of Information Science & Electronic Engineering, Zhejiang University, Hangzhou 310027, China;
2.
² Suzhou Wei Mu Intelligent System Ltd., Suzhou 215163, China

Corresponding author: 汪小知

Received Date: 06 September 2015
Fund Project:
本文系国家自然科学基金青年基金(No.61301046)

浙江省公益性技术应用研究计划项目(No.2013C31023)

Abstract: Field asymmetric ion mobility spectrometry(FAIMS) technology was used for the rapid analysis of phenylalanine(PHE) using a metal diffusion tube-microchip FAIMS system. The test pressure was set at 250 kPa, and the temperature of the gas generator for the diffusion tube was at 190℃. The ion characteristic spectra were acquired under the optimized conditions with a gas flow rate of 2000 mL/min, a dispersion voltage of 152.8 V and a compensation voltage value of-0.62 V for phenylalanine in positive mode. Phenylalanine gas samples with different concentrations were analyzed by FAIMS. The results showed that the ion intensity was linearly correlated to phenylalanine concentration in the range from 6 mg/L to 20 mg/L, and the limit of detection for phenylalanine was 5.9 mg/L. This investigation shows the feasibility of FAIMS technique for rapid detection of phenylalanine. The FAIMS instrument is simple and sensitive, and can be used for rapid detection of phenylalanine.

Key words:

Phenylalanine
/ Rapid Determination
/ Field asymmetric ion mobility spectrometry
/ Metal diffusion tube

1. [1]
  1 Pey A L, Desviat L R, Gámez A, Ugarte M, Pérez B. Hum. Mutat., 2003, 21(4):370-3781 Pey A L, Desviat L R, Gámez A, Ugarte M, Pérez B. Hum. Mutat., 2003, 21(4):370-378
2. [2]
  2 YE Jun, LIU Xiao-Qing, MA Xie-Qin, ZHANG Ya-Fen, HUANG Xiao-Dong, CHEN Rui-Guan, GU Xue-Fan. Chinese Med. J.-Peking, 2002, 115(2):217-221 叶军, 刘晓青, 马燮琴, 张雅芬, 黄晓东, 陈瑞冠, 顾学范. 中华医学杂志英文版, 2002, 115(2):217-2212 YE Jun, LIU Xiao-Qing, MA Xie-Qin, ZHANG Ya-Fen, HUANG Xiao-Dong, CHEN Rui-Guan, GU Xue-Fan. Chinese Med. J.-Peking, 2002, 115(2):217-221 叶军, 刘晓青, 马燮琴, 张雅芬, 黄晓东, 陈瑞冠, 顾学范. 中华医学杂志英文版, 2002, 115(2):217-221
3. [3]
  3 Halaban R, Cheng E, Hebert D N. J. Invest. Dermatol., 2002, 119(2):481-4883 Halaban R, Cheng E, Hebert D N. J. Invest. Dermatol., 2002, 119(2):481-488
4. [4]
  4 Morrow B A, Elsworth J D, Roth R H. Synapse, 1996, 22(2):100-1054 Morrow B A, Elsworth J D, Roth R H. Synapse, 1996, 22(2):100-105
5. [5]
  5 HONG Min, TANG Ai-Guo. J. Cent. South Univ.(Med Sci), 2004, 29(1):67-71 洪敏, 唐爱国. 中南大学学报(医学版), 2004, 29(1):67-715 HONG Min, TANG Ai-Guo. J. Cent. South Univ.(Med Sci), 2004, 29(1):67-71 洪敏, 唐爱国. 中南大学学报(医学版), 2004, 29(1):67-71
6. [6]
  6 QIN Li-Xin, HONG Min, TANG Ai-Guo, MO Xi-Ming. Pract Prevent Med., 2006, 12(3):529-531 秦立新, 洪敏, 唐爱国, 莫喜明. 实用预防医学, 2006, 12(3):529-5316 QIN Li-Xin, HONG Min, TANG Ai-Guo, MO Xi-Ming. Pract Prevent Med., 2006, 12(3):529-531 秦立新, 洪敏, 唐爱国, 莫喜明. 实用预防医学, 2006, 12(3):529-531
7. [7]
  7 HUANG Xian-Zhi, XU Jin-Gou, LI Yao-Qun. Chinese J. Anal. Chem., 1987, 15(3):199-202 黄贤智, 许金钩, 李耀群. 分析化学, 1987, 15(3):199-2027 HUANG Xian-Zhi, XU Jin-Gou, LI Yao-Qun. Chinese J. Anal. Chem., 1987, 15(3):199-202 黄贤智, 许金钩, 李耀群. 分析化学, 1987, 15(3):199-202
8. [8]
  8 CAO Wei, FU Pei-Yu. Spectrosc. Spect. Anal., 1995, 15(2):119-122 曹伟, 付佩玉. 光谱学与光谱分析, 1995, 15(2):119-1228 CAO Wei, FU Pei-Yu. Spectrosc. Spect. Anal., 1995, 15(2):119-122 曹伟, 付佩玉. 光谱学与光谱分析, 1995, 15(2):119-122
9. [9]
  9 CAI Sheng, TU Chun-Yan, ZOU Jia-Qin, YANG Dong-Hong. Journal of Nanjing University of Chemical Technology, 1999, 21(3):39-43 蔡升, 屠春燕, 邹家庆, 杨冬红. 南京化工大学学报, 1999, 21(3):39-439 CAI Sheng, TU Chun-Yan, ZOU Jia-Qin, YANG Dong-Hong. Journal of Nanjing University of Chemical Technology, 1999, 21(3):39-43 蔡升, 屠春燕, 邹家庆, 杨冬红. 南京化工大学学报, 1999, 21(3):39-43
10. [10]
  10 CHEN Yong-Bo, RAO Bin. Amino. Acids & Biotic Resources., 2000, 22(1):55-58 陈永波, 饶斌. 氨基酸和生物资源, 2000, 22(1):55-5810 CHEN Yong-Bo, RAO Bin. Amino. Acids & Biotic Resources., 2000, 22(1):55-58 陈永波, 饶斌. 氨基酸和生物资源, 2000, 22(1):55-58
11. [11]
  11 Armstrong M, Jonscher K, Reisdorph N A. Rapid Commun. Mass Spectrom., 2007, 21(16):2717-272611 Armstrong M, Jonscher K, Reisdorph N A. Rapid Commun. Mass Spectrom., 2007, 21(16):2717-2726
12. [12]
  12 McCooeye M, Mester Z. Rapid Commun. Mass Spectrom., 2006, 20(11):1801-180812 McCooeye M, Mester Z. Rapid Commun. Mass Spectrom., 2006, 20(11):1801-1808
13. [13]
  13 Alarcón-Flores M I, Romero-González R, Frenich A G, Vidal J L M, Reyes R C. Anal. Methods., 2010, 2(11):1745-175113 Alarcón-Flores M I, Romero-González R, Frenich A G, Vidal J L M, Reyes R C. Anal. Methods., 2010, 2(11):1745-1751
14. [14]
  14 Buryakov I A, Krylov E V, Nazarov E G, Rasulev U K. Int. J. Mass Spectrom. Ion Process., 1993, 128(3):143-14814 Buryakov I A, Krylov E V, Nazarov E G, Rasulev U K. Int. J. Mass Spectrom. Ion Process., 1993, 128(3):143-148
15. [15]
  15 LIU You-Jiang, CHEN Chi-Lai, ZHANG Xiao-Tian, WANG Hong-Wei, ZHANG Le-Hua, KONG De-Yi. Chinese J. Anal.Chem., 2014, 42(9):1259-1263 刘友江, 陈池来, 张乐华, 张晓天, 王泓伟, 孔德义. 分析化学, 2014, 42(9):1259-126315 LIU You-Jiang, CHEN Chi-Lai, ZHANG Xiao-Tian, WANG Hong-Wei, ZHANG Le-Hua, KONG De-Yi. Chinese J. Anal.Chem., 2014, 42(9):1259-1263 刘友江, 陈池来, 张乐华, 张晓天, 王泓伟, 孔德义. 分析化学, 2014, 42(9):1259-1263
16. [16]
  16 Cohen M J, Karasek F W. J. Chromatogr. Sci., 1970, 8(6):330-33716 Cohen M J, Karasek F W. J. Chromatogr. Sci., 1970, 8(6):330-337
17. [17]
  17 Guevremont R. J. Chromatogr. A, 2004, 1058(1-2):3-1917 Guevremont R. J. Chromatogr. A, 2004, 1058(1-2):3-19
18. [18]
  18 Prieto M, Yost R A. Int. J. Ion Mobil. Spectrom., 2011, 14(2-3):61-6918 Prieto M, Yost R A. Int. J. Ion Mobil. Spectrom., 2011, 14(2-3):61-69
19. [19]
  19 Shvartsburg A A, Seim T A, Danielson W F, Norheim R, Moore R J, Anderson G A, Smith R D. J. Am. Soc. Mass Spectrom., 2013, 24(1):109-11419 Shvartsburg A A, Seim T A, Danielson W F, Norheim R, Moore R J, Anderson G A, Smith R D. J. Am. Soc. Mass Spectrom., 2013, 24(1):109-114
20. [20]
  20 Nazarov E G. Int. J. Ion Mobil. Spectrom., 2012, 15(3):83-8420 Nazarov E G. Int. J. Ion Mobil. Spectrom., 2012, 15(3):83-84
21. [21]
  21 Cumeras R, Gràcia I, Figueras E, Fonseca L, Santander J, Salleras M, Calaza C, Sabaté N, Cané C. Sen. Actuat B:Chem., 2012, 170:13-2021 Cumeras R, Gràcia I, Figueras E, Fonseca L, Santander J, Salleras M, Calaza C, Sabaté N, Cané C. Sen. Actuat B:Chem., 2012, 170:13-20
22. [22]
  22 ZHANG Jie, LI Ling-Feng, GUO Da-Peng, ZHANG Yuan, WANG Qi, LI Peng, WANG Xiao-Zhi. Chinese J. Anal. Chem., 2013, 41(7):986-992 张洁, 李灵锋, 郭大鹏, 张媛, 王琦, 李鹏, 汪小知. 分析化学, 2013, 41(7):986-99222 ZHANG Jie, LI Ling-Feng, GUO Da-Peng, ZHANG Yuan, WANG Qi, LI Peng, WANG Xiao-Zhi. Chinese J. Anal. Chem., 2013, 41(7):986-992 张洁, 李灵锋, 郭大鹏, 张媛, 王琦, 李鹏, 汪小知. 分析化学, 2013, 41(7):986-992
23. [23]
  23 Guo D, Wang Y, Li L, Wang X, Luo J. J. Mass Spectrom., 2015, 50(1):198-20523 Guo D, Wang Y, Li L, Wang X, Luo J. J. Mass Spectrom., 2015, 50(1):198-205
24. [24]
  24 Shvartsburg A A, Smith R D. Anal. Chem., 2012, 85(1):10-1324 Shvartsburg A A, Smith R D. Anal. Chem., 2012, 85(1):10-13
25. [25]
  25 O Donnell R M, Sun X, Harrington P D B. TRAC-Trends Anal. Chem., 2008, 27(1):44-5325 O Donnell R M, Sun X, Harrington P D B. TRAC-Trends Anal. Chem., 2008, 27(1):44-53
26. [26]
  26 LI Zhuang, LIN Bing-Tao, CHEN Chi-Lai, LIU Ying, KONG De-Yi, CHENG Yu-Peng, WANG Dian-Ling, WANG Huan-Qin. Chinese J. Anal. Chem., 2011, 39(1):107-110 李庄, 林丙涛, 陈池来, 刘英, 孔德义, 程玉鹏, 王电令, 王焕钦. 分析化学, 2011, 39(1):107-11026 LI Zhuang, LIN Bing-Tao, CHEN Chi-Lai, LIU Ying, KONG De-Yi, CHENG Yu-Peng, WANG Dian-Ling, WANG Huan-Qin. Chinese J. Anal. Chem., 2011, 39(1):107-110 李庄, 林丙涛, 陈池来, 刘英, 孔德义, 程玉鹏, 王电令, 王焕钦. 分析化学, 2011, 39(1):107-110
27. [27]
  27 LIN Bing-Tao, CHEN Chi-Lai, KONG De-Yi, LI Zhuang, WANG Huan-Qin, CHENG Yu-Peng, WANG Dian-Ling, MEI Tao. Chinese J. Anal. Chem., 2010, 38(7):1027-1030 林丙涛, 陈池来, 孔德义, 李庄, 王焕钦, 程玉鹏, 王电令, 梅涛. 分析化学, 2010, 38(7):1027-103027 LIN Bing-Tao, CHEN Chi-Lai, KONG De-Yi, LI Zhuang, WANG Huan-Qin, CHENG Yu-Peng, WANG Dian-Ling, MEI Tao. Chinese J. Anal. Chem., 2010, 38(7):1027-1030 林丙涛, 陈池来, 孔德义, 李庄, 王焕钦, 程玉鹏, 王电令, 梅涛. 分析化学, 2010, 38(7):1027-1030
28. [28]
  28 Tsai C, Yost R A, Garrett T J. Bioanalysis, 2012, 4(11):1363-137528 Tsai C, Yost R A, Garrett T J. Bioanalysis, 2012, 4(11):1363-1375
29. [29]
  29 McCooeye M, Mester Z. Rapid Commun. Mass Spectrom., 2006, 20(11):1801-180829 McCooeye M, Mester Z. Rapid Commun. Mass Spectrom., 2006, 20(11):1801-1808
30. [30]
  30 Xia Y, Wu S T, Jemal M. Anal. Chem., 2008, 80(18):7137-714330 Xia Y, Wu S T, Jemal M. Anal. Chem., 2008, 80(18):7137-7143
31. [31]
  31 Wu S T, Xia Y Q, Jemal M. Rapid Commun. Mass Spectrom., 2007, 21(22):3667-367631 Wu S T, Xia Y Q, Jemal M. Rapid Commun. Mass Spectrom., 2007, 21(22):3667-3676
32. [32]
  32 LI Ling-Feng, WANG Tie-Song, HAN Ke, XU Tian-Bai, WANG Qi, LI Peng, WANG Xiao-Zhi. Chinese J. Anal. Chem., 2014, 42(4):519-524 李灵锋, 王铁松, 韩可, 徐天白, 王琦, 李鹏, 汪小知. 分析化学, 2014, 42(4):519-52432 LI Ling-Feng, WANG Tie-Song, HAN Ke, XU Tian-Bai, WANG Qi, LI Peng, WANG Xiao-Zhi. Chinese J. Anal. Chem., 2014, 42(4):519-524 李灵锋, 王铁松, 韩可, 徐天白, 王琦, 李鹏, 汪小知. 分析化学, 2014, 42(4):519-524
33. [33]
  33 LI Juan, ZHAO Wei-Jun, LI Ling-Feng, LI Xin, LI Peng, WANG Xiao-Zhi. Chem. J. Chinese Universities, 2014, 35(7):1403-1408 李娟, 赵伟军, 李灵锋, 李鑫, 李鹏, 汪小知. 高等学校化学学报, 2014, 35(7):1403-140833 LI Juan, ZHAO Wei-Jun, LI Ling-Feng, LI Xin, LI Peng, WANG Xiao-Zhi. Chem. J. Chinese Universities, 2014, 35(7):1403-1408 李娟, 赵伟军, 李灵锋, 李鑫, 李鹏, 汪小知. 高等学校化学学报, 2014, 35(7):1403-1408
34. [34]
  34 Wilks A, Hart M, Koehl A, Somerville J, Boyle B, Ruiz-Alonso D. Int. J. Ion Mobil. Spectrom., 2012, 15(3):199-22234 Wilks A, Hart M, Koehl A, Somerville J, Boyle B, Ruiz-Alonso D. Int. J. Ion Mobil. Spectrom., 2012, 15(3):199-222

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

利用芯片式高场非对称波形离子迁移谱技术快速检测苯丙氨酸

通讯作者: 汪小知

English

Rapid Determination of Phenylalanine by Micro-chip based Field Asymmetric Waveform Ion Mobility Spectrometry

Corresponding author: 汪小知

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

利用芯片式高场非对称波形离子迁移谱技术快速检测苯丙氨酸

通讯作者: 汪小知

English

Rapid Determination of Phenylalanine by Micro-chip based Field Asymmetric Waveform Ion Mobility Spectrometry

Corresponding author: 汪小知

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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