引用本文:
Rong CHEN, Pin YANG, Chun Ying WEI. La3+ Transmembrane Research in Guinea Pig Ventricular Cells by Fura-2 Fluorescence[J]. Chinese Chemical Letters,
2000, 11(8): 725-726.
Citation: Rong CHEN, Pin YANG, Chun Ying WEI. La3+ Transmembrane Research in Guinea Pig Ventricular Cells by Fura-2 Fluorescence[J]. Chinese Chemical Letters, 2000, 11(8): 725-726.
Citation: Rong CHEN, Pin YANG, Chun Ying WEI. La3+ Transmembrane Research in Guinea Pig Ventricular Cells by Fura-2 Fluorescence[J]. Chinese Chemical Letters, 2000, 11(8): 725-726.
La3+ Transmembrane Research in Guinea Pig Ventricular Cells by Fura-2 Fluorescence
摘要:
Binding of La3+ to Fura-2 can change 340/380 nm fluorescence intensity ratio. Whether La3+ cross ventricular cell membrane was detected by this fluorescent probe technique. Fura-2 loaded isolated guinea pig ventricular cells were exposed to 0.01-0.1mM extracellular Lanthanum ion concentration, 340nm/380 nm fluorescence ratio was not changed. Using calcium channel agonist BAY K8644, KCL (35mM) depolarization to open the voltage-dependent calcium channel (VDCC); Adrenoceptor agonist to excite adrenoceptor, 340/380 ratio was not changed, suggesting that La3+ can not enter guinea pig ventricular cells in this case.
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关键词:
- Ventricular cells
- / adrenaline
- / La3+
- / transmembrane
- / Fura-2
- / BAY K8644
English
La3+ Transmembrane Research in Guinea Pig Ventricular Cells by Fura-2 Fluorescence
Abstract:
Binding of La3+ to Fura-2 can change 340/380 nm fluorescence intensity ratio. Whether La3+ cross ventricular cell membrane was detected by this fluorescent probe technique. Fura-2 loaded isolated guinea pig ventricular cells were exposed to 0.01-0.1mM extracellular Lanthanum ion concentration, 340nm/380 nm fluorescence ratio was not changed. Using calcium channel agonist BAY K8644, KCL (35mM) depolarization to open the voltage-dependent calcium channel (VDCC); Adrenoceptor agonist to excite adrenoceptor, 340/380 ratio was not changed, suggesting that La3+ can not enter guinea pig ventricular cells in this case.
-
Key words:
- Ventricular cells
- / adrenaline
- / La3+
- / transmembrane
- / Fura-2
- / BAY K8644
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