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微流控PCR芯片的研究进展

何启迪 ,  黄丹萍 ,  黄冠 ,  陈缵光

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引用本文: 何启迪, 黄丹萍, 黄冠, 陈缵光. 微流控PCR芯片的研究进展[J]. 分析化学, 2016, 44(4): 542-550. doi: 10.11895/j.issn.0253-3820.160058 shu
Citation:  HE Qi-Di, HUANG Dan-Ping, HUANG Guan, CHEN Zuan-Guang. Advance in Research of Microfluidic Polymerase Chain Reaction Chip[J]. Chinese Journal of Analytical Chemistry, 2016, 44(4): 542-550. doi: 10.11895/j.issn.0253-3820.160058 shu

微流控PCR芯片的研究进展

  • 1.

    中山大学药学院, 广州 510006

    • 通讯作者: 陈缵光
  • 基金项目:

    本文系国家自然科学基金(No.21375152)资助 (No.21375152)

摘要: 基因是人类的遗传密码,人类个体之间只有万分之一的基因不相同,却导致了人与人之间丰富的差异。了解这种差异对于科学研究具有巨大的应用价值。PCR是基因研究中常用的手段之一,但传统PCR仪存在反应时间长、能量消耗大、不便于集成与携带等缺陷,微流控技术与PCR结合可以有效缩小反应体系,提高反应效率,且易于集成化与微型化。本文按照微流控PCR芯片的结构分类,详细介绍了微池型、连续流动型PCR芯片,以及电泳、荧光、电化学和DNA杂交阵列等检测方法,并在最后进行了总结与展望。

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  • 收稿日期:  2016-01-21
  • 网络出版日期:  2016-02-25
通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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