Isolation, purification and structural characterization of a water-soluble polysaccharide HM<sub>41</sub> from <i>Halenia elliptica</i> D. Don

引用本文: Chun-Lan Liu, Yang Li, Gui-Yun Xu, Ya-Shuang Li. Isolation, purification and structural characterization of a water-soluble polysaccharide HM₄₁ from Halenia elliptica D. Don[J]. Chinese Chemical Letters, 2016, 27(6): 979-983. doi: 10.1016/j.cclet.2016.01.061 shu

Citation: Chun-Lan Liu, Yang Li, Gui-Yun Xu, Ya-Shuang Li. Isolation, purification and structural characterization of a water-soluble polysaccharide HM₄₁ from Halenia elliptica D. Don[J]. Chinese Chemical Letters, 2016, 27(6): 979-983. doi: 10.1016/j.cclet.2016.01.061 shu

Isolation, purification and structural characterization of a water-soluble polysaccharide HM₄₁ from Halenia elliptica D. Don

a College of Life and Environmental Science, Minzu University of China, Beijing 100081, China;
b Beijing Engineering Research Center of Food Environment and Public Health, Minzu University of China, Beijing 10008l, China;
c Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China
基金项目:
This work was financially supported by the First-Class Discipline Construction Project for First-Class University of Minzu University of China (No. YLDX01013), 111 Project (No. B08044) and The ecological conservation technology integration and demonstration in north of Dzungaria (No. 2014BAC15B04).

摘要: A water-soluble polysaccharide, HM₄₁, was obtained from Halenia elliptica D. Don by acidic ethanol fractionation and gel filtration. Its homogeneity was confirmed by chromatography using multiple systems. HM₄₁ was composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal), glucose (Glc) with a molar ratio of 1.0:5.5:1.8:3.0:9.4:21. The average molecular weight of HM₄₁ was approximately 1.17 × 10⁴ . Periodate oxidation, Smith degradation, methylation and GC, IR, NMR, XRD, GC-MS analysis were used for the structural analysis of HM₄₁. Its main chain was composed mainly of β-(1→4)Gal, β-(1→4)Glc and β-(1→6)Glc. β-(1→4)Gal were substituted at 6-O and on average there were 14 branches among 23 main chain residues; (1→4)Glc had no branch; (1→6)Glc were substituted at 3-O and on average there were 9 branches among 14 main chain residues. The side chain was composed of (1→3,6)-Rha, (1→4)/(1→5)-Ara, (1→4)/(1→5)-Xyl, (1→4,6)-Man and (1→2)-Glc. The terminal residue was composed of Ara, Xyl, Man, Gal, and Glc. Then, we demonstrated that HM and HM₄₁ had strong scavenging activities in vitro hydroxyl. Overall, HM and HM₄₁ may have potential applications in the antioxidants for medical and food industry.

关键词:

English

Isolation, purification and structural characterization of a water-soluble polysaccharide HM₄₁ from Halenia elliptica D. Don

a College of Life and Environmental Science, Minzu University of China, Beijing 100081, China;
b Beijing Engineering Research Center of Food Environment and Public Health, Minzu University of China, Beijing 10008l, China;
c Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China
Received Date: 19 November 2015
Revised Date: 20 January 2016
Fund Project:
This work was financially supported by the First-Class Discipline Construction Project for First-Class University of Minzu University of China (No. YLDX01013), 111 Project (No. B08044) and The ecological conservation technology integration and demonstration in north of Dzungaria (No. 2014BAC15B04).

Abstract: A water-soluble polysaccharide, HM₄₁, was obtained from Halenia elliptica D. Don by acidic ethanol fractionation and gel filtration. Its homogeneity was confirmed by chromatography using multiple systems. HM₄₁ was composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal), glucose (Glc) with a molar ratio of 1.0:5.5:1.8:3.0:9.4:21. The average molecular weight of HM₄₁ was approximately 1.17 × 10⁴ . Periodate oxidation, Smith degradation, methylation and GC, IR, NMR, XRD, GC-MS analysis were used for the structural analysis of HM₄₁. Its main chain was composed mainly of β-(1→4)Gal, β-(1→4)Glc and β-(1→6)Glc. β-(1→4)Gal were substituted at 6-O and on average there were 14 branches among 23 main chain residues; (1→4)Glc had no branch; (1→6)Glc were substituted at 3-O and on average there were 9 branches among 14 main chain residues. The side chain was composed of (1→3,6)-Rha, (1→4)/(1→5)-Ara, (1→4)/(1→5)-Xyl, (1→4,6)-Man and (1→2)-Glc. The terminal residue was composed of Ara, Xyl, Man, Gal, and Glc. Then, we demonstrated that HM and HM₄₁ had strong scavenging activities in vitro hydroxyl. Overall, HM and HM₄₁ may have potential applications in the antioxidants for medical and food industry.

Key words:

1. [1] H.L. Yang, J.Q. Liu, Seven constituents in nine species used as Tibetan medicine "Zangyinchen", China Tradit. Herb Drugs 36 (2005) 1233-1237.
2. [2] D. Zhang, Y.F. Zhu, S.K. Lin, Identification of new chemical constituents of Tibetan medicinal herb Halenia elliptica, China Tradit. Herb Drugs 34 (2003) 9-11.
3. [3] X.Y. Qin, L. Yang, L.M. Shao, S.L. Liu, Inducement of the Callus of Halenia elliptica D. Don in Sichuan Province, Li Shi Zhen Med. Materia Med. Res. 19 (2008) 35-39.
4. [4] Y. Liu, J. Zhao, Preliminary study on the physicochemical properties of the polysaccharide from Portobello Mushroom, Hubei Agric. Sci. 51 (2012) 981-983.
5. [5] Y.J. Liu, H.Y. Zhu, Optimization of de-protein process for polysaccharide of Hippophae rhamnoides, J. Henan Agric. Sci. 3 (2008) 84-87.
6. [6] N.S. Li, C.Y. Yan, D.H. Hua, et al., Isolation, purification, and structural characterization of a novel polysaccharide from Ganoderma capense, Int. J. Biol. Macromol. 57 (2013) 285-290.
7. [7] M. Dubois, K.A. Gilles, J.K. Hamilton, et al., Colorimetric method for determination of sugars and related substances, Anal. Chem. 28 (1956) 350-360.
8. [8] T. Li, Q.H. Zhang, W.B. Zhang, High Performance Liquid Chromatography (HPLC) Instrument System, Chemical Industry Press, Beijing, 2009, pp. 204-215.
9. [9] G.Y. Xu, R.X. Cheng, L.W. Chang, Component analysis of monosaccharides in polysaccharides by capillary gas chromatography, J. Instrumental Anal. 19 (2000) 71-73.
10. [10] Q. Dong, X. Liu, J. Yao, Structural characterization of a pectic polysaccharide from Nerium indicum flowers, Phytochemistry 71 (2010) 1430-1437.
11. [11] Q. Li, Y. Xie, J.W. Su, et al., Isolation and structural characterization of a neutral polysaccharide from the stems of Dendrobium densiflorum, Int. J. Biol. Macromol. 50 (2012) 1207-1211.
12. [12] R.A. Kalyan, B.T. Paul, A comprehensive procedure for preparation of partially methylated alditol acetates from glycoprotein carbohydrates, Anal. Biochem. 203 (1992) 101-108.
13. [13] A. Linker, L.R. Evans, G. Impallomeni, The structure of a polysaccharide from infectious strains of Burkholderia cepacia, Carbohydr. Res. 335 (2001) 45-54.
14. [14] G.L. Sassaki, P.A.J. Gorin, L.M. Souza, et al., Rapid synthesis of partially O-methylated alditol acetate standards for GC-MS: some relative activities of hydroxyl groups of methyl glycopyranosides on Purdie methylation, Carbohydr. Res. 340 (2005) 731-739.
15. [15] M.T. Duenas-Chasco, M.A. Rodriguez-Carvajal, P.T. Mateo, et al., Structural analysis of the exopolysaccharides produced by Lactobacillus spp. G-77, Carbohydr. Res. 307 (1998) 125-133.
16. [16] S.L. Wu, S.C. Xie, L. Yang, et al., An experimental study on the production efficiency of hydroxyl radicals by several photocatalysis reaction systems, Photogr. Sci. Photochem. 2 (2006) 75-78.
17. [17] D.R. Grymonpre, A.K. Sharma, W.C. Finney, The role of Fenton's reaction in aqueous phase pulsed streamer corona reactors, Chem. Eng. J. 82 (2001) 189-207.
18. [18] F.X. Liu, Z.D. Li, N. Li, et al., Determination techniques introduction on hydroxyl radical in Fenton method, Guangdong Chem. Ind. 23 (2007) 35-38.
19. [19] Y.L. Wu, Y.J. Pan, C.R. Sun, Structural analysis of an alkali-extractable polysaccharide from the seeds of Retama raetamssp, Gussonei. Nat. Prod. 69 (2006) 1109-1112.
20. [20] B. La, K. Xie, C.X. Hu, Studies on X-ray Fourier spectrum of Halenia elliptica D. Don, Qinghai Med. J. 34 (2004) 53-54.
21. [21] Y.L. Sun, F. Shan, W.W. Cui, et al., NMR analysis of polysaccharide ASP3 from Angelica sinensis (Oliv.) Diels and it's hydrolysis products, Chem. J Chin. Univ. 30 (2009) 1739-1743.
22. [22] Z. Wu, H. Li, D.W. Tu, et al., Extraction optimization, preliminary characterization, and in vitro antioxidant, Ind. Crop. Prod. 44 (2013) 145-151.
23. [23] H. Zdenka, S.P. Berit, P. Martin, et al., Structural features of two heteroxylan polysaccharide fractions from wheat bran with anti-complementary and antioxidant activities, Carbohydr. Polym. 93 (2013) 22-30.

扫一扫看文章

计量

PDF下载量: 0
文章访问数: 1420
HTML全文浏览量: 29

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation, purification and structural characterization of a water-soluble polysaccharide HM₄₁ from Halenia elliptica D. Don

English

Isolation, purification and structural characterization of a water-soluble polysaccharide HM₄₁ from Halenia elliptica D. Don

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

Isolation, purification and structural characterization of a water-soluble polysaccharide HM41 from Halenia elliptica D. Don

English

Isolation, purification and structural characterization of a water-soluble polysaccharide HM41 from Halenia elliptica D. Don

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

Isolation, purification and structural characterization of a water-soluble polysaccharide HM₄₁ from Halenia elliptica D. Don

Isolation, purification and structural characterization of a water-soluble polysaccharide HM₄₁ from Halenia elliptica D. Don

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs