引用本文:
Li Ming DU, Zhe Feng FAN, Jin Li QIAO, Jing Ping WANG. Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization[J]. Chinese Chemical Letters,
2001, 12(11): 1007-1010.
Citation: Li Ming DU, Zhe Feng FAN, Jin Li QIAO, Jing Ping WANG. Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization[J]. Chinese Chemical Letters, 2001, 12(11): 1007-1010.
Citation: Li Ming DU, Zhe Feng FAN, Jin Li QIAO, Jing Ping WANG. Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization[J]. Chinese Chemical Letters, 2001, 12(11): 1007-1010.
Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization
摘要:
Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%~95.7% and the RSD is 1.2%~4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.
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关键词:
- Sparfloxacin
- / HPLC
- / pre-column derivatization
- / human urine
English
Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization
Abstract:
Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%~95.7% and the RSD is 1.2%~4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.
-
Key words:
- Sparfloxacin
- / HPLC
- / pre-column derivatization
- / human urine
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