Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization

Li Ming DU Zhe Feng FAN Jin Li QIAO Jing Ping WANG

引用本文: Li Ming DU,  Zhe Feng FAN,  Jin Li QIAO,  Jing Ping WANG. Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization[J]. Chinese Chemical Letters, 2001, 12(11): 1007-1010. shu
Citation:  Li Ming DU,  Zhe Feng FAN,  Jin Li QIAO,  Jing Ping WANG. Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization[J]. Chinese Chemical Letters, 2001, 12(11): 1007-1010. shu

Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization

  • 基金项目:

    This work was supported by the Natural Science Foundation of Shanxi Province (No. 20011016).

摘要: Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%~95.7% and the RSD is 1.2%~4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.

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  • 收稿日期:  2001-05-27
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